A0KJ20 (TGT_AERHH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Queuine tRNA-ribosyltransferase
EC=2.4.2.29

Alternative name(s):
Guanine insertion enzyme
tRNA-guanine transglycosylase

Gene names

Name:	tgt
Ordered Locus Names:	AHA_1735

Organism

Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) [Complete proteome] [HAMAP]

Taxonomic identifier

380703 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	378 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity. HAMAP MF_00168
Catalytic activity	[tRNA]-guanine + queuine = [tRNA]-queuine + guanine. HAMAP MF_00168 [tRNA]-guanine + 7-aminomethyl-7-carbaguanine = [tRNA]-7-aminomethyl-7-carbaguanine + guanine. HAMAP MF_00168
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00168
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00168
Sequence similarities	Belongs to the queuine tRNA-ribosyltransferase family.

Ontologies

Keywords
Biological process	Queuosine biosynthesis tRNA processing
Ligand	Metal-binding Zinc
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	queuosine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW queuine tRNA-ribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 378	378	Queuine tRNA-ribosyltransferase HAMAP MF_00168		PRO_1000016760
Sites
	Active site	89	1	Nucleophile By similarity
	Metal binding	302	1	Zinc By similarity
	Metal binding	304	1	Zinc By similarity
	Metal binding	307	1	Zinc By similarity
	Metal binding	333	1	Zinc By similarity
	Binding site	90	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0KJ20 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 7E8AC59F6BA129B4	FASTA	378	43,001
	10 20 30 40 50 60 MKFELKTTDG RARRGQLVFE RGTVQTPAFM PVGTYGTVKG MTPEEVRETG AQILLGNTFH 70 80 90 100 110 120 LWLRPGQEVM RAHGDLHDFM NWQGPILTDS GGFQVFSLGH IRKITEAGVH FRHPINGEKI 130 140 150 160 170 180 FLDPEKSMEI QYDLGSDIVM IFDECTPYPA TYEEARKSME MSLRWGKRSR DKFDALGNKN 190 200 210 220 230 240 ALFGIIQGSV YEDLRDVSLD GLLEIGFDGY AVGGLAVGEP KEDMHRILEH VCPKIPADKP 250 260 270 280 290 300 RYLMGVGKPE DLVEGVRRGI DMFDCVMPTR NARNGHLFTT DGVVKIRNAK YREDTSTLDA 310 320 330 340 350 360 DCDCYTCKNY TRSYLYHLDK CNEILGARLN TIHNLRYYQR VMQGLRDAIE QGKLDDFVTE 370 FYRRQGKPVP PLAENDVK

References

[1]

"Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades." Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F. J. Bacteriol. 188:8272-8282(2006) [PubMed: 16980456] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 7966 / NCIB 9240.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000462 Genomic DNA. Translation: ABK37967.1.
RefSeq	YP_856271.1. NC_008570.1.
3D structure databases
ProteinModelPortal	A0KJ20.
SMR	A0KJ20. Positions 1-364.
ModBase	Search...
Protein-protein interaction databases
STRING	A0KJ20.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4490304.
GenomeReviews	Gene locus AHA_1735 in contig CP000462_GR.
KEGG	aha:AHA_1735.
PATRIC	20781048. VBIAerHyd135212_1750.
TIGR	AHA_1735.
Phylogenomic databases
eggNOG	COG0343.
HOGENOM	HBG629929.
OMA	FMPVGTV.
PhylomeDB	A0KJ20.
ProtClustDB	PRK00112.
Enzyme and pathway databases
BioCyc	AHYD196024:AHA_1735-MONOMER.
Family and domain databases
HAMAP	MF_00168. Q_tRNA_Tgt. [Tree]
InterPro	IPR004803. Queuine_tRNA-ribosylTrfase. IPR002616. tRNA_ribo_trans. [Graphical view]
Gene3D	G3DSA:3.20.20.105. tRNA_ribo_trans. 1 hit.
KO	K00773.
PANTHER	PTHR11962. tRNA_ribo_trans. 1 hit.
Pfam	PF01702. TGT. 1 hit. [Graphical view]
SUPFAM	SSF51713. tRNA_ribo_trans. 1 hit.
TIGRFAMs	TIGR00430. Q_tRNA_tgt. 1 hit. TIGR00449. Tgt_general. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TGT_AERHH

Accession

Primary (citable) accession number: A0KJ20

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	December 12, 2006
Last modified:	December 14, 2011
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program