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A0KIN9 (FTHS_AERHH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Formate--tetrahydrofolate ligase

EC=6.3.4.3
Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS
Gene names
Name:fhs
Ordered Locus Names:AHA_1604
OrganismAeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) [Complete proteome] [HAMAP]
Taxonomic identifier380703 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeAeromonas

Protein attributes

Sequence length576 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543

Sequence similarities

Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
   Biological processOne-carbon metabolism
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processfolic acid-containing compound biosynthetic process

Inferred from electronic annotation. Source: InterPro

tetrahydrofolate interconversion

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

formate-tetrahydrofolate ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 576576Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543
PRO_0000300514

Regions

Nucleotide binding64 – 718ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A0KIN9 [UniParc].

Last modified December 12, 2006. Version 1.
Checksum: BF9395B9F24F6500

FASTA57660,860
        10         20         30         40         50         60 
MLSDIEISRQ SPRLPIHALA ERLAIPSHQL HPYGHYKGKL SLELLKSLPK PAGKLVLVSA 

        70         80         90        100        110        120 
ITPTPLGEGK TVTTLGLSMG LNHIGQPSIA TIRQPSLGPV FGVKGGAAGG GHAQVVPMEE 

       130        140        150        160        170        180 
MNLHLTGDFH ALTAAHNLAA AALDARLFHE RKLGDQFSAR TGLQRLDIDA DNILWPRTLD 

       190        200        210        220        230        240 
MNDRALRHLT IGQGNAADGV ERSDRFVITA ASELMAILAL ASDLKDLRQR IGRIQLARDL 

       250        260        270        280        290        300 
RGKPITAEQL EVAGAMTVLL KEALQPTLMQ TTEQTSVLVH AGPFANIAHG NSSVIADRMA 

       310        320        330        340        350        360 
LGLTDYVVTE AGFGSDMGLE KFFNIKHRQS GITPACVVLV ATVRGLKANS GLLDIRPGQP 

       370        380        390        400        410        420 
LPESLLREDL PTLEQGCANL GWHIANARRY GVPVVVAINR FPTDSEAELA LLARAALRAG 

       430        440        450        460        470        480 
ACGSAISSAF TQGGAGASEL AQAVVAACEQ PGKVKLLYPD QASLGAKLAT LVECGYGGRG 

       490        500        510        520        530        540 
VQLSDKARQQ LAQLSAEGWD HLPVCVAKTP LSISHDPALK GVPTDFEVPI EEVKLCAGAG 

       550        560        570 
FVYALAGPIM TMPGLGSLPA YRHIDIDDNG EIVGLS 

« Hide

References

[1]"Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades."
Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F.
J. Bacteriol. 188:8272-8282(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 7966 / NCIB 9240.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000462 Genomic DNA. Translation: ABK36840.1.
RefSeqYP_856140.1. NC_008570.1.

3D structure databases

ProteinModelPortalA0KIN9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING380703.AHA_1604.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABK36840; ABK36840; AHA_1604.
GeneID4488356.
KEGGaha:AHA_1604.
PATRIC20780780. VBIAerHyd135212_1616.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2759.
HOGENOMHOG000040280.
KOK01938.
OMACGEIMTM.
OrthoDBEOG6PCPSP.
ProtClustDBPRK13506.

Enzyme and pathway databases

BioCycAHYD380703:GH2M-1604-MONOMER.
UniPathwayUPA00193.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
HAMAPMF_01543. FTHFS.
InterProIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFTHS_AERHH
AccessionPrimary (citable) accession number: A0KIN9
Entry history
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: December 12, 2006
Last modified: April 16, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways