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A0KIM1 (NAPA_AERHH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:AHA_1586
OrganismAeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) [Complete proteome] [HAMAP]
Taxonomic identifier380703 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeAeromonas

Protein attributes

Sequence length829 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3030Tat-type signal Potential
Chain31 – 829799Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_1000069707

Regions

Domain41 – 97574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding481Iron-sulfur (4Fe-4S) By similarity
Metal binding511Iron-sulfur (4Fe-4S) By similarity
Metal binding551Iron-sulfur (4Fe-4S) By similarity
Metal binding831Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
A0KIM1 [UniParc].

Last modified December 12, 2006. Version 1.
Checksum: CA3A2CB2674ED4BE

FASTA82992,843
        10         20         30         40         50         60 
MKLSRRDFMK ANAVAAAAAV AGVSAPTLAA NLITSTDKTA ITWDKAPCRF CGTGCSVLVG 

        70         80         90        100        110        120 
SQDGRVVATQ GDPDAPVNRG LNCIKGYFLS KIMYGQDRLT QPMLRMTDGK FDKNGNFAPI 

       130        140        150        160        170        180 
SWDQAFDIMA EKFKTTLKEK GPTAVGMFGS GQWTVWEGYA AAKLMKAGLR TNNLDPNARH 

       190        200        210        220        230        240 
CMASAVVGFM RTFGMDEPMG CYDDIEQADA FVLWGSNMAE MHPILWSRIS DRRLSHQDVQ 

       250        260        270        280        290        300 
VHVLSTFEHR SFELADNGMV FTPQTDLAIL NYIANYIIQN DKVNWEFVNK HTQFRKGVTD 

       310        320        330        340        350        360 
IGYGLRPTNP LQQKAKNPDS GDSTPMSFDD FAKFVADYDL ESVSKLSGVP KDKLEKLAQL 

       370        380        390        400        410        420 
YADPSKKVVS YWTMGFNQHT RGVWANNLCY NIHLLTGKIS TPGSGPFSLT GQPSACGTAR 

       430        440        450        460        470        480 
EVGTFAHRLP ADMVVTEPKH RAIAEKIWKL PEGTIPEQVG YHAVLQNRML KDGKLNAYWV 

       490        500        510        520        530        540 
MCNNNMQAGP NMNEEGLPGY RNPANFIVVS DPYPTVTAQA ADLILPTAMW VEKEGAYGNA 

       550        560        570        580        590        600 
ERRTQFWHQQ VKPPEGAKSD LWQLMEFSKR FKVEEVWPAE LIAKMPEVKG KTLFDVLYAN 

       610        620        630        640        650        660 
GQVNQFPKEQ SKGALNDEAE HFGFYVQKGL FEEYATFGRG HGHDLAPFDQ YHEARGLRWP 

       670        680        690        700        710        720 
VVDGKETLWR YREGFDPYVK AGEGVRFYGK PDGKAVIFAL PYEPAAEAPD KEYDMWLSTG 

       730        740        750        760        770        780 
RVLEHWHTGT MTRRVPELYR AFPDAVLFMH PEDAKARGVR RGEEVIVSSR RGEVKTRVET 

       790        800        810        820 
RGRNRPPKGL VFMPFFDASQ LVNKLTLDAT DPLSKETDYK KCAVKVVKA 

« Hide

References

[1]"Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades."
Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F.
J. Bacteriol. 188:8272-8282(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 7966 / NCIB 9240.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000462 Genomic DNA. Translation: ABK35881.1.
RefSeqYP_856122.1. NC_008570.1.

3D structure databases

ProteinModelPortalA0KIM1.
SMRA0KIM1. Positions 40-828.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING380703.AHA_1586.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABK35881; ABK35881; AHA_1586.
GeneID4486851.
KEGGaha:AHA_1586.
PATRIC20780744. VBIAerHyd135212_1598.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMAQMVYNLH.
OrthoDBEOG6CVV7G.

Enzyme and pathway databases

BioCycAHYD380703:GH2M-1586-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_AERHH
AccessionPrimary (citable) accession number: A0KIM1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: December 12, 2006
Last modified: May 14, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families