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A0KIM1

- NAPA_AERHH

UniProt

A0KIM1 - NAPA_AERHH

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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster.UniRule annotation
Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi48 – 481Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi51 – 511Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi55 – 551Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi83 – 831Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciAHYD380703:GH2M-1586-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:AHA_1586
OrganismiAeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240)
Taxonomic identifieri380703 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeAeromonas
ProteomesiUP000000756: Chromosome

Subcellular locationi

Periplasm UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030Tat-type signalUniRule annotationAdd
BLAST
Chaini31 – 829799Periplasmic nitrate reductasePRO_1000069707Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Interactioni

Subunit structurei

Interacts with NapB.UniRule annotation

Protein-protein interaction databases

STRINGi380703.AHA_1586.

Structurei

3D structure databases

ProteinModelPortaliA0KIM1.
SMRiA0KIM1. Positions 40-828.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini41 – 97574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiQMVYNLH.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A0KIM1-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MKLSRRDFMK ANAVAAAAAV AGVSAPTLAA NLITSTDKTA ITWDKAPCRF
60 70 80 90 100
CGTGCSVLVG SQDGRVVATQ GDPDAPVNRG LNCIKGYFLS KIMYGQDRLT
110 120 130 140 150
QPMLRMTDGK FDKNGNFAPI SWDQAFDIMA EKFKTTLKEK GPTAVGMFGS
160 170 180 190 200
GQWTVWEGYA AAKLMKAGLR TNNLDPNARH CMASAVVGFM RTFGMDEPMG
210 220 230 240 250
CYDDIEQADA FVLWGSNMAE MHPILWSRIS DRRLSHQDVQ VHVLSTFEHR
260 270 280 290 300
SFELADNGMV FTPQTDLAIL NYIANYIIQN DKVNWEFVNK HTQFRKGVTD
310 320 330 340 350
IGYGLRPTNP LQQKAKNPDS GDSTPMSFDD FAKFVADYDL ESVSKLSGVP
360 370 380 390 400
KDKLEKLAQL YADPSKKVVS YWTMGFNQHT RGVWANNLCY NIHLLTGKIS
410 420 430 440 450
TPGSGPFSLT GQPSACGTAR EVGTFAHRLP ADMVVTEPKH RAIAEKIWKL
460 470 480 490 500
PEGTIPEQVG YHAVLQNRML KDGKLNAYWV MCNNNMQAGP NMNEEGLPGY
510 520 530 540 550
RNPANFIVVS DPYPTVTAQA ADLILPTAMW VEKEGAYGNA ERRTQFWHQQ
560 570 580 590 600
VKPPEGAKSD LWQLMEFSKR FKVEEVWPAE LIAKMPEVKG KTLFDVLYAN
610 620 630 640 650
GQVNQFPKEQ SKGALNDEAE HFGFYVQKGL FEEYATFGRG HGHDLAPFDQ
660 670 680 690 700
YHEARGLRWP VVDGKETLWR YREGFDPYVK AGEGVRFYGK PDGKAVIFAL
710 720 730 740 750
PYEPAAEAPD KEYDMWLSTG RVLEHWHTGT MTRRVPELYR AFPDAVLFMH
760 770 780 790 800
PEDAKARGVR RGEEVIVSSR RGEVKTRVET RGRNRPPKGL VFMPFFDASQ
810 820
LVNKLTLDAT DPLSKETDYK KCAVKVVKA
Length:829
Mass (Da):92,843
Last modified:December 12, 2006 - v1
Checksum:iCA3A2CB2674ED4BE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000462 Genomic DNA. Translation: ABK35881.1.
RefSeqiYP_856122.1. NC_008570.1.

Genome annotation databases

EnsemblBacteriaiABK35881; ABK35881; AHA_1586.
GeneIDi4486851.
KEGGiaha:AHA_1586.
PATRICi20780744. VBIAerHyd135212_1598.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000462 Genomic DNA. Translation: ABK35881.1 .
RefSeqi YP_856122.1. NC_008570.1.

3D structure databases

ProteinModelPortali A0KIM1.
SMRi A0KIM1. Positions 40-828.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 380703.AHA_1586.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABK35881 ; ABK35881 ; AHA_1586 .
GeneIDi 4486851.
KEGGi aha:AHA_1586.
PATRICi 20780744. VBIAerHyd135212_1598.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OMAi QMVYNLH.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

BioCyci AHYD380703:GH2M-1586-MONOMER.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 7966 / NCIB 9240.

Entry informationi

Entry nameiNAPA_AERHH
AccessioniPrimary (citable) accession number: A0KIM1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: December 12, 2006
Last modified: October 29, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3