Reviewed, UniProtKB/Swiss-Prot A0KIM1 (NAPA_AERHH)

Names and origin

Protein names

Recommended name:
    Periplasmic nitrate reductase
    EC=1.7.99.4

Gene names

Name:	napA
Ordered Locus Names:	AHA_1586

Organism

Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) [Complete proteome] [HAMAP]

Taxonomic identifier

380703 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	829 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity.
Catalytic activity	Nitrite + acceptor = nitrate + reduced acceptor. HAMAP MF_01630
Cofactor	Binds 1 4Fe-4S cluster By similarity. Binds 1 molybdenum ion per subunit By similarity. Binds 2 molybdopterin guanine dinucleotide (MGD) groups per subunit By similarity.
Subunit structure	Interacts with napB By similarity.
Subcellular location	Periplasm By similarity.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP MF_01630
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily.

Ontologies

Keywords
Biological process	Electron transport Nitrate assimilation Transport
Cellular component	Periplasm
Domain	Signal
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Molybdenum
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	Mo-molybdopterin cofactor biosynthetic process Inferred from electronic annotation. Source: HAMAP electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW nitrate assimilation Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP molybdenum ion binding Inferred from electronic annotation. Source: UniProtKB-KW nitrate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	Tat-type signal Potential
	Chain	31 – 829	799	Periplasmic nitrate reductase HAMAP MF_01630		PRO_1000069707
Sites
	Metal binding	48	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	51	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	55	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	83	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0KIM1-1 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: CA3A2CB2674ED4BE	FASTA	829	92,843
	10 20 30 40 50 60 MKLSRRDFMK ANAVAAAAAV AGVSAPTLAA NLITSTDKTA ITWDKAPCRF CGTGCSVLVG 70 80 90 100 110 120 SQDGRVVATQ GDPDAPVNRG LNCIKGYFLS KIMYGQDRLT QPMLRMTDGK FDKNGNFAPI 130 140 150 160 170 180 SWDQAFDIMA EKFKTTLKEK GPTAVGMFGS GQWTVWEGYA AAKLMKAGLR TNNLDPNARH 190 200 210 220 230 240 CMASAVVGFM RTFGMDEPMG CYDDIEQADA FVLWGSNMAE MHPILWSRIS DRRLSHQDVQ 250 260 270 280 290 300 VHVLSTFEHR SFELADNGMV FTPQTDLAIL NYIANYIIQN DKVNWEFVNK HTQFRKGVTD 310 320 330 340 350 360 IGYGLRPTNP LQQKAKNPDS GDSTPMSFDD FAKFVADYDL ESVSKLSGVP KDKLEKLAQL 370 380 390 400 410 420 YADPSKKVVS YWTMGFNQHT RGVWANNLCY NIHLLTGKIS TPGSGPFSLT GQPSACGTAR 430 440 450 460 470 480 EVGTFAHRLP ADMVVTEPKH RAIAEKIWKL PEGTIPEQVG YHAVLQNRML KDGKLNAYWV 490 500 510 520 530 540 MCNNNMQAGP NMNEEGLPGY RNPANFIVVS DPYPTVTAQA ADLILPTAMW VEKEGAYGNA 550 560 570 580 590 600 ERRTQFWHQQ VKPPEGAKSD LWQLMEFSKR FKVEEVWPAE LIAKMPEVKG KTLFDVLYAN 610 620 630 640 650 660 GQVNQFPKEQ SKGALNDEAE HFGFYVQKGL FEEYATFGRG HGHDLAPFDQ YHEARGLRWP 670 680 690 700 710 720 VVDGKETLWR YREGFDPYVK AGEGVRFYGK PDGKAVIFAL PYEPAAEAPD KEYDMWLSTG 730 740 750 760 770 780 RVLEHWHTGT MTRRVPELYR AFPDAVLFMH PEDAKARGVR RGEEVIVSSR RGEVKTRVET 790 800 810 820 RGRNRPPKGL VFMPFFDASQ LVNKLTLDAT DPLSKETDYK KCAVKVVKA

References

[1]

"Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades." Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F. J. Bacteriol. 188:8272-8282(2006) [PubMed: 16980456] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000462 Genomic DNA. Translation: ABK35881.1.
RefSeq	YP_856122.1.
3D structure databases
SMR	A0KIM1. Positions 40-828.
ModBase	Search...
Genome annotation databases
GeneID	4486851.
GenomeReviews	Gene locus AHA_1586 in contig CP000462_GR.
KEGG	aha:AHA_1586.
TIGR	AHA_1586.
Phylogenomic databases
OMA	A0KIM1. KAGLRTN.
Family and domain databases
HAMAP	MF_01630. [Tree]
InterPro	IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4. IPR006655. Mopterin_OxRdtase_prok_CS. IPR006657. MPT_dinuc_bd. IPR010051. NO3_reductase_lsu_periplasm. IPR006311. Tat. IPR019546. TAT_signal. IPR017909. Twin_arg_translocation_Tat. [Graphical view]
Pfam	PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. PF10518. TAT_signal. 1 hit. [Graphical view]
TIGRFAMs	TIGR01706. NAPA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. 1 hit. PS00490. MOLYBDOPTERIN_PROK_2. False negative. PS00932. MOLYBDOPTERIN_PROK_3. False negative. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAPA_AERHH

Accession

Primary (citable) accession number: A0KIM1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	December 12, 2006
Last modified:	June 16, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)