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A0KIC9 (BIOD_AERHH) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
ATP-dependent dethiobiotin synthetase BioD
EC=6.3.3.3
Alternative name(s):
DTB synthetase
Short name=DTBS
Dethiobiotin synthase
Gene names
Name:bioD
Ordered Locus Names:AHA_1492
OrganismAeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) [Complete proteome] [HAMAP]
Taxonomic identifier380703 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length226 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336

Catalytic activity

ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336

Cofactor

Magnesium By similarity. HAMAP MF_00336

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336

Subcellular location

Cytoplasm By similarity HAMAP MF_00336.

Sequence similarities

Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
   Biological processBiotin biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processbiotin biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

dethiobiotin synthase activity

Inferred from electronic annotation. Source: EC

magnesium ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 226226ATP-dependent dethiobiotin synthetase BioD HAMAP MF_00336
PRO_1000205208

Regions

Nucleotide binding13 – 186ATP By similarity
Nucleotide binding117 – 1204ATP By similarity
Nucleotide binding177 – 1782ATP By similarity
Nucleotide binding206 – 2083ATP By similarity

Sites

Metal binding131Magnesium 1 By similarity
Metal binding171Magnesium 2 By similarity
Metal binding551Magnesium 2 By similarity
Metal binding1171Magnesium 2 By similarity
Binding site551ATP By similarity
Binding site2131ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A0KIC9 [UniParc].

Last modified December 12, 2006. Version 1.
Checksum: B66060C48384F2ED

FASTA22624,448
        10         20         30         40         50         60 
MVKSFFVTGT DTDVGKTLVA RTLLLEFAAH GLRCAGYKPI SAGCARTPDG LRNLDAVLLQ 

        70         80         90        100        110        120 
EAASLPLPYD LVNPYAYEPP IAPHIAASEA RDAITLKGLS DGLRQIEQAG AELVVVEGAG 

       130        140        150        160        170        180 
GWFLPLDRKH LLSDWVKQEN MPVIMVVGAK LGCLNHALLT FAAIRNNNLP VAGWVINRLH 

       190        200        210        220 
GSMSHYQENL DTLRGLLPAP FLGEIPFVNN PLEADLRGRL DISPLL

« Hide

References

[1]"Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades."
Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F.
J. Bacteriol. 188:8272-8282(2006) [PubMed: 16980456] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 7966 / NCIB 9240.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000462 Genomic DNA. Translation: ABK38307.1.
RefSeqYP_856030.1. NC_008570.1.

3D structure databases

ProteinModelPortalA0KIC9.
ModBaseSearch...

Protein-protein interaction databases

STRINGA0KIC9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4487693.
GenomeReviewsGene locus AHA_1492 in contig CP000462_GR.
KEGGaha:AHA_1492.
PATRIC20780550. VBIAerHyd135212_1503.
TIGRAHA_1492.

Phylogenomic databases

eggNOGCOG0132.
HOGENOMHBG650065.
OMAANCVDPN.
PhylomeDBA0KIC9.
ProtClustDBCLSK729654.

Enzyme and pathway databases

BioCycAHYD196024:AHA_1492-MONOMER.

Family and domain databases

HAMAPMF_00336. BioD.
[Tree]
InterProIPR002586. CbiA_P_synth.
IPR004472. DTB_synth_BioD.
[Graphical view]
KOK01935.
PfamPF01656. CbiA. 1 hit.
[Graphical view]
PIRSFPIRSF006755. DTB_synth. 1 hit.
TIGRFAMsTIGR00347. BioD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOD_AERHH
AccessionPrimary (citable) accession number: A0KIC9
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: December 12, 2006
Last modified: December 14, 2011
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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