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A0KIC6

- BIOB_AERHH

UniProt

A0KIC6 - BIOB_AERHH

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Protein

Biotin synthase

Gene
bioB, AHA_1489
Organism
Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi61 – 611Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi65 – 651Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi68 – 681Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi105 – 1051Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi136 – 1361Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi196 – 1961Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi268 – 2681Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciAHYD380703:GH2M-1489-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:AHA_1489
OrganismiAeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240)
Taxonomic identifieri380703 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeAeromonas
ProteomesiUP000000756: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 362362Biotin synthaseUniRule annotationPRO_0000381184Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi380703.AHA_1489.

Structurei

3D structure databases

ProteinModelPortaliA0KIC6.
SMRiA0KIC6. Positions 12-322.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OMAiRIMMPAS.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

A0KIC6-1 [UniParc]FASTAAdd to Basket

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MNAVTAGAHA LRHDWTLAEV QALFALPFND LLFQAQTVHR AHFDPNEVQV    50
STLLSIKTGA CPEDCKYCPQ SARYHTGLEA ERLMEVEKVL ERAREAKANG 100
SSRFCMGAAW RNPKEKDMPY ILRMIEEVRG LGMETCMTLG MLTADQAARL 150
GAAGLDYYNH NLDTSPEFYG DIITTRTYQD RLDTLAYVRG AGMKVCSGGI 200
VGMGEQAKDR AGLLMALANL PRHPESVPIN MLVKVKGTPL ENQENLDPFE 250
FIRTIAVARI MMPASHVRLS AGREKMNEQM QAMCFMAGAN SIFYGCKLLT 300
TPNPDENSDM QLFKRLGIRP AQRAQKPDQV QEEELLAEVS RQSEPAEMFY 350
DATRPRAGAA RS 362
Length:362
Mass (Da):40,295
Last modified:December 12, 2006 - v1
Checksum:i559F1245EF2252EC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000462 Genomic DNA. Translation: ABK36458.1.
RefSeqiWP_011705387.1. NC_008570.1.
YP_856027.1. NC_008570.1.

Genome annotation databases

EnsemblBacteriaiABK36458; ABK36458; AHA_1489.
GeneIDi4487690.
KEGGiaha:AHA_1489.
PATRICi20780544. VBIAerHyd135212_1500.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000462 Genomic DNA. Translation: ABK36458.1 .
RefSeqi WP_011705387.1. NC_008570.1.
YP_856027.1. NC_008570.1.

3D structure databases

ProteinModelPortali A0KIC6.
SMRi A0KIC6. Positions 12-322.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 380703.AHA_1489.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABK36458 ; ABK36458 ; AHA_1489 .
GeneIDi 4487690.
KEGGi aha:AHA_1489.
PATRICi 20780544. VBIAerHyd135212_1500.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OMAi RIMMPAS.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci AHYD380703:GH2M-1489-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 7966 / NCIB 9240.

Entry informationi

Entry nameiBIOB_AERHH
AccessioniPrimary (citable) accession number: A0KIC6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: December 12, 2006
Last modified: September 3, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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