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A0KIC3 (SYN_AERHH) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Asparagine--tRNA ligase
EC=6.1.1.22
Alternative name(s):
Asparaginyl-tRNA synthetase
Short name=AsnRS
Gene names
Name:asnS
Ordered Locus Names:AHA_1486
OrganismAeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) [Complete proteome] [HAMAP]
Taxonomic identifier380703 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length466 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534

Subunit structure

Homodimer By similarity. HAMAP MF_00534

Subcellular location

Cytoplasm HAMAP MF_00534.

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processasparaginyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

asparagine-tRNA ligase activity

Inferred from electronic annotation. Source: EC

nucleic acid binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 466466Asparagine--tRNA ligase HAMAP MF_00534
PRO_1000051374

Sequences

Sequence LengthMass (Da)Tools
A0KIC3 [UniParc].

Last modified December 12, 2006. Version 1.
Checksum: 14222D4576FB6E2E

FASTA46652,215
        10         20         30         40         50         60 
MTHASVVDVL TGKYAVGTTQ TVKGWIRTRR DSKAGISFLA ISDGSCFHPV QAVVPNTLAN 

        70         80         90        100        110        120 
YENEVLRLTT ACSVEVTGVV AASQGSGQAF ELQATEVKVV GWVEDPDTYP MAAKRHSIEY 

       130        140        150        160        170        180 
LREQGHLRAR TNMVGAVTRV RNCLSQAIHR FFHEQGYLWI AAPLITASDT EGAGEMFRVS 

       190        200        210        220        230        240 
TLDMENLPRT PAGKVDYDKD FFGKETFLTV SGQLNVETYA CALSKVYTFG PTFRAENSNT 

       250        260        270        280        290        300 
SRHLAEFWMV EPEVAFADLE DNAALAEAML KYVFNAVLAE RRDDLEFFAQ HVDKDAIGRL 

       310        320        330        340        350        360 
ERFVASDFAQ IDYTDAIEVL KNCGKKFEFP VSWGIDLSSE HERYLAEEHF KSPVVVKNYP 

       370        380        390        400        410        420 
KDIKAFYMRL NDDGKTVAAM DVLAPGIGEI IGGSQREERL DVLDARLAEM GLNKEDYWWY 

       430        440        450        460 
RDLRRYGTVP HSGFGLGFER LVVYVTGMGN VRDVIPFPRT PRTAEF

« Hide

References

[1]"Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades."
Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F.
J. Bacteriol. 188:8272-8282(2006) [PubMed: 16980456] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 7966 / NCIB 9240.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000462 Genomic DNA. Translation: ABK38069.1.
RefSeqYP_856024.1. NC_008570.1.

3D structure databases

ProteinModelPortalA0KIC3.
ModBaseSearch...

Protein-protein interaction databases

STRINGA0KIC3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4487687.
GenomeReviewsGene locus AHA_1486 in contig CP000462_GR.
KEGGaha:AHA_1486.
PATRIC20780534. VBIAerHyd135212_1495.
TIGRAHA_1486.

Phylogenomic databases

eggNOGCOG0017.
HOGENOMHBG745843.
OMAAIHRFFH.
PhylomeDBA0KIC3.
ProtClustDBPRK03932.

Enzyme and pathway databases

BioCycAHYD196024:AHA_1486-MONOMER.

Family and domain databases

HAMAPMF_00534. Asn_tRNA_synth.
[Tree]
InterProIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004522. Asn-tRNA-synth_IIb.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR004365. NA-bd_OB_tRNA-helicase.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KOK01893.
PANTHERPTHR22594. aa-tRNA-synt_II. 1 hit.
PTHR22594:SF6. PTHR22594:SF6. 1 hit.
PfamPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti. 1 hit.
[Graphical view]
PRINTSPR01042. TRNASYNTHASP.
SUPFAMSSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMsTIGR00457. AsnS. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYN_AERHH
AccessionPrimary (citable) accession number: A0KIC3
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: December 12, 2006
Last modified: January 25, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

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