A0KHV5 (RLMF_AERHH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase F
EC=2.1.1.181

Alternative name(s):
23S rRNA mA1618 methyltransferase
rRNA adenine N-6-methyltransferase

Gene names

Name:	rlmF
Ordered Locus Names:	AHA_1315

Organism

Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) [Complete proteome] [HAMAP]

Taxonomic identifier

380703 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	318 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the adenine in position 1618 of 23S rRNA By similarity. HAMAP MF_01848
Catalytic activity	S-adenosyl-L-methionine + adenine(1618) in 23S rRNA = S-adenosyl-L-homocysteine + rRNA containing N(6)-methyladenine(1618) in 23S rRNA. HAMAP MF_01848
Subcellular location	Cytoplasm Potential HAMAP MF_01848.
Sequence similarities	Belongs to the methyltransferase superfamily. METTL16/rlmF family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	rRNA (adenine-N6-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 318	318	Ribosomal RNA large subunit methyltransferase F HAMAP MF_01848		PRO_0000349893

Sequences

	Sequence		Length	Mass (Da)
	A0KHV5 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 41B54FDC8C852499	FASTA	318	34,686
	10 20 30 40 50 60 MKTHSDRKSG LHPRNRHQAP YDFDALCQRT PELQPFVFVN EHGTQTLDFA DPAAVKALNK 70 80 90 100 110 120 ALLALHYGIA HWDLPAGYLC PPIPGRVDYL HRVADLLAES AGKVPTGKGV RVLDIGVGAN 130 140 150 160 170 180 CIYPLLGARE YGWRFVGSDI DPVSVKAATL LAKSNGLGSQ IECRLQGKAG DIFQGIVAQR 190 200 210 220 230 240 ERFALTLCNP PFHASLAEAS KGTERKLRNL GKEATAKPVL NFGGQKAELW CEGGEAAFLA 250 260 270 280 290 300 SMITQSKAFA SQCLWFSSLV SKKENLPAAK QGLSRIGARQ VRVLEMAQGN KISRVLAWSF 310 QDEAASRQWW SPMAHDNA

References

[1]

"Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades." Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F. J. Bacteriol. 188:8272-8282(2006) [PubMed: 16980456] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 7966 / NCIB 9240.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000462 Genomic DNA. Translation: ABK38867.1.
RefSeq	YP_855856.1. NC_008570.1.
3D structure databases
ProteinModelPortal	A0KHV5.
ModBase	Search...
Protein-protein interaction databases
STRING	A0KHV5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4488445.
GenomeReviews	Gene locus AHA_1315 in contig CP000462_GR.
KEGG	aha:AHA_1315.
PATRIC	20780190. VBIAerHyd135212_1325.
TIGR	AHA_1315.
Phylogenomic databases
eggNOG	COG3129.
HOGENOM	HBG747880.
OMA	WSFVGSD.
PhylomeDB	A0KHV5.
ProtClustDB	PRK11727.
Enzyme and pathway databases
BioCyc	AHYD196024:AHA_1315-MONOMER.
Family and domain databases
HAMAP	MF_01848. 23SrRNA_methyltr_F. [Tree]
InterPro	IPR016909. rRNA_lsu_methyltransferase_F. IPR010286. SAM-Mtase_prd. [Graphical view]
KO	K06970.
PANTHER	PTHR13393. DUF890. 1 hit.
Pfam	PF05971. Methyltransf_10. 1 hit. [Graphical view]
PIRSF	PIRSF029038. Mtase_YbiN_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMF_AERHH

Accession

Primary (citable) accession number: A0KHV5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	December 12, 2006
Last modified:	December 14, 2011
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program