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Protein

Crossover junction endodeoxyribonuclease RuvC

Gene

ruvC

Organism
Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi8MagnesiumUniRule annotation1
Metal bindingi67MagnesiumUniRule annotation1
Metal bindingi139MagnesiumUniRule annotation1
Metal bindingi142MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RuvCUniRule annotation (EC:3.1.22.4UniRule annotation)
Alternative name(s):
Holliday junction nuclease RuvCUniRule annotation
Holliday junction resolvase RuvCUniRule annotation
Gene namesi
Name:ruvCUniRule annotation
Ordered Locus Names:AHA_1301
OrganismiAeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240)
Taxonomic identifieri380703 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeAeromonas
Proteomesi
  • UP000000756 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000027101 – 173Crossover junction endodeoxyribonuclease RuvCAdd BLAST173

Interactioni

Protein-protein interaction databases

STRINGi380703.AHA_1301.

Structurei

3D structure databases

ProteinModelPortaliA0KHU1.
SMRiA0KHU1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvC family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105NHU. Bacteria.
COG0817. LUCA.
HOGENOMiHOG000012181.
KOiK01159.
OMAiFFNRNVT.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00034. RuvC. 1 hit.
InterProiIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamiPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSiPR00696. RSOLVASERUVC.
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00228. ruvC. 1 hit.
PROSITEiPS01321. RUVC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0KHU1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIILGIDPG SRITGYGVIR IVAGKAEYLG SGCIRTDLGE LPSRLKQVYD
60 70 80 90 100
GVSEIITQFK PDEFAIERVF MARNADSALK LGQARGSAIV AAVNALLPVS
110 120 130 140 150
EYSATQIKQA VVGTGGAAKE QVQHMVTHLL KLSATPQADA ADALGVALCH
160 170
FHTRQILIKM AGRSTGSVRG RYR
Length:173
Mass (Da):18,393
Last modified:December 12, 2006 - v1
Checksum:i6D83331A5FB9167D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000462 Genomic DNA. Translation: ABK39733.1.
RefSeqiWP_005298649.1. NC_008570.1.
YP_855842.1. NC_008570.1.

Genome annotation databases

EnsemblBacteriaiABK39733; ABK39733; AHA_1301.
GeneIDi28663944.
4487389.
KEGGiaha:AHA_1301.
PATRICi20780160. VBIAerHyd135212_1310.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000462 Genomic DNA. Translation: ABK39733.1.
RefSeqiWP_005298649.1. NC_008570.1.
YP_855842.1. NC_008570.1.

3D structure databases

ProteinModelPortaliA0KHU1.
SMRiA0KHU1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi380703.AHA_1301.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK39733; ABK39733; AHA_1301.
GeneIDi28663944.
4487389.
KEGGiaha:AHA_1301.
PATRICi20780160. VBIAerHyd135212_1310.

Phylogenomic databases

eggNOGiENOG4105NHU. Bacteria.
COG0817. LUCA.
HOGENOMiHOG000012181.
KOiK01159.
OMAiFFNRNVT.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00034. RuvC. 1 hit.
InterProiIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamiPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSiPR00696. RSOLVASERUVC.
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00228. ruvC. 1 hit.
PROSITEiPS01321. RUVC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRUVC_AERHH
AccessioniPrimary (citable) accession number: A0KHU1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: December 12, 2006
Last modified: November 2, 2016
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.