ID   MAO1_AERHH              Reviewed;         564 AA.
AC   A0KHR8;
DT   18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT   18-MAR-2008, sequence version 2.
DT   16-JUN-2009, entry version 24.
DE   RecName: Full=NAD-dependent malic enzyme;
DE            Short=NAD-ME;
DE            EC=1.1.1.38;
GN   Name=sfcA; OrderedLocusNames=AHA_1278;
OS   Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Aeromonadales;
OC   Aeromonadaceae; Aeromonas.
OX   NCBI_TaxID=380703;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16980456; DOI=10.1128/JB.00621-06;
RA   Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J.,
RA   Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M.,
RA   Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F.;
RT   "Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all
RT   trades.";
RL   J. Bacteriol. 188:8272-8282(2006).
CC   -!- CATALYTIC ACTIVITY: (S)-malate + NAD(+) = pyruvate + CO(2) + NADH.
CC   -!- COFACTOR: Divalent metal cations. Prefers magnesium or manganese
CC       (By similarity).
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SIMILARITY: Belongs to the malic enzymes family.
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DR   EMBL; CP000462; ABK38619.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; YP_855819.1; -.
DR   GeneID; 4490711; -.
DR   GenomeReviews; CP000462_GR; AHA_1278.
DR   KEGG; aha:AHA_1278; -.
DR   TIGR; AHA_1278; -.
DR   GO; GO:0016619; F:malate dehydrogenase (oxaloacetate-decarbox...; IEA:HAMAP.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0051287; F:NAD or NADH binding; IEA:InterPro.
DR   GO; GO:0006108; P:malate metabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_01619; -; 1.
DR   InterPro; IPR015884; Malic_enzyme_CS.
DR   InterPro; IPR012301; Malic_N.
DR   InterPro; IPR012302; Malic_NAD_bd.
DR   InterPro; IPR001891; Malic_OxRdtase.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF00390; malic; 1.
DR   Pfam; PF03949; Malic_M; 1.
DR   PRINTS; PR00072; MALOXRDTASE.
DR   PROSITE; PS00331; MALIC_ENZYMES; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Metal-binding; NAD; Oxidoreductase.
FT   CHAIN         1    564       NAD-dependent malic enzyme.
FT                                /FTId=PRO_0000323535.
FT   ACT_SITE    104    104       Proton donor (By similarity).
FT   ACT_SITE    175    175       Proton acceptor (By similarity).
FT   METAL       246    246       Divalent metal cation (By similarity).
FT   METAL       247    247       Divalent metal cation (By similarity).
FT   METAL       270    270       Divalent metal cation (By similarity).
FT   BINDING     157    157       NAD (By similarity).
FT   BINDING     270    270       NAD (By similarity).
FT   BINDING     417    417       NAD (By similarity).
FT   SITE        270    270       Important for activity (By similarity).
SQ   SEQUENCE   564 AA;  62671 MW;  270B94F4609283F3 CRC64;
     MFEDNNQKRP LYIPYAGPAL LETPLLNKGS AFTSEERSNF NLEGLLPQNI ETIEEQAERA
     YRQFMAFGND MDKHIYLRNI QDTNETLFYR LLTNHLTEML PVIYTPTVGK ACEEFSNIYR
     RARGLFISYP DKDRIDDMLQ NATKQNVKVI VVTDGERILG LGDQGIGGMG IPIGKLSLYT
     ACGGISPAYC LPVVLDVGTN NQQLLNDPFY MGWRNPRISG EEYAEFVDAF IQAVKRRWPD
     ILLQFEDFAQ NNAMPLLNRY KNELCCFNDD IQGTAAVTLG SLIAACKASG AKLSEKRVAF
     LGAGSAGCGI AEQIVAQMKA EGLTDAQARS RVFMVDRFGL ITDKIPNQLD FQRRLSQPVE
     RIADWPVGDN ISLLEVMEHG RPDILIGVSG QPGLFTEEVV KTMHKHCARP IIFPLSNPTS
     RVEATPADLI RWTDGQALVA TGSPFAPVEY KGKRYVIAQC NNSFIFPGIG LGVIAAGATR
     VTDAMLMSAS RALAECSPLV KGEEGSLLPD LADIHQVSRY IAKMVAKTAM LQGKAVQTPD
     EVIDQSIEAN FWRPEYRRYR RTSF
//