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Reviewed, UniProtKB/Swiss-Prot A0KHR8 (MAO1_AERHH)

Last modified June 16, 2009. Version 24. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    NAD-dependent malic enzyme
      Short name=NAD-ME
    EC=1.1.1.38
Gene names
Name: sfcA
Ordered Locus Names: AHA_1278
OrganismAeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) [Complete proteome] [HAMAP]
Taxonomic identifier380703 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

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Protein attributes

Sequence length564 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Catalytic activity

(S)-malate + NAD+ = pyruvate + CO2 + NADH. HAMAP MF_01619

Cofactor

Divalent metal cations. Prefers magnesium or manganese By similarity.

Subunit structure

Homotetramer By similarity.

Sequence similarities

Belongs to the malic enzymes family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 564564NAD-dependent malic enzyme HAMAP MF_01619
PRO_0000323535

Sites

Active site1041Proton donor By similarity
Active site1751Proton acceptor By similarity
Metal binding2461Divalent metal cation By similarity
Metal binding2471Divalent metal cation By similarity
Metal binding2701Divalent metal cation By similarity
Binding site1571NAD By similarity
Binding site2701NAD By similarity
Binding site4171NAD By similarity
Site2701Important for activity By similarity

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Sequences

Sequence LengthMass (Da)Tools
A0KHR8-1 [UniParc].

Last modified March 18, 2008. Version 2.
Checksum: 270B94F4609283F3

FASTA56462,671
        10         20         30         40         50         60 
MFEDNNQKRP LYIPYAGPAL LETPLLNKGS AFTSEERSNF NLEGLLPQNI ETIEEQAERA 

        70         80         90        100        110        120 
YRQFMAFGND MDKHIYLRNI QDTNETLFYR LLTNHLTEML PVIYTPTVGK ACEEFSNIYR 

       130        140        150        160        170        180 
RARGLFISYP DKDRIDDMLQ NATKQNVKVI VVTDGERILG LGDQGIGGMG IPIGKLSLYT 

       190        200        210        220        230        240 
ACGGISPAYC LPVVLDVGTN NQQLLNDPFY MGWRNPRISG EEYAEFVDAF IQAVKRRWPD 

       250        260        270        280        290        300 
ILLQFEDFAQ NNAMPLLNRY KNELCCFNDD IQGTAAVTLG SLIAACKASG AKLSEKRVAF 

       310        320        330        340        350        360 
LGAGSAGCGI AEQIVAQMKA EGLTDAQARS RVFMVDRFGL ITDKIPNQLD FQRRLSQPVE 

       370        380        390        400        410        420 
RIADWPVGDN ISLLEVMEHG RPDILIGVSG QPGLFTEEVV KTMHKHCARP IIFPLSNPTS 

       430        440        450        460        470        480 
RVEATPADLI RWTDGQALVA TGSPFAPVEY KGKRYVIAQC NNSFIFPGIG LGVIAAGATR 

       490        500        510        520        530        540 
VTDAMLMSAS RALAECSPLV KGEEGSLLPD LADIHQVSRY IAKMVAKTAM LQGKAVQTPD 

       550        560 
EVIDQSIEAN FWRPEYRRYR RTSF

« Hide

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References

[1]"Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades."
Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F.
J. Bacteriol. 188:8272-8282(2006) [PubMed: 16980456] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases

CP000462 Genomic DNA. Translation: ABK38619.1. Different initiation.
RefSeqYP_855819.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID4490711.
GenomeReviewsGene locus AHA_1278 in contig CP000462_GR.
KEGGaha:AHA_1278.
TIGRAHA_1278.

Family and domain databases

HAMAPMF_01619.
[Tree]
InterProIPR015884. Malic_enzyme_CS.
IPR012301. Malic_N.
IPR012302. Malic_NAD_bd.
IPR001891. Malic_OxRdtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PfamPF00390. malic. 1 hit.
PF03949. Malic_M. 1 hit.
[Graphical view]
PRINTSPR00072. MALOXRDTASE.
PROSITEPS00331. MALIC_ENZYMES. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameMAO1_AERHH
AccessionPrimary (citable) accession number: A0KHR8
Entry history
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 18, 2008
Last modified: June 16, 2009
This is version 24 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents