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Protein

DNA polymerase IV

Gene

dinB

Organism
Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII.UniRule annotation

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi8 – 81MagnesiumUniRule annotation
Sitei13 – 131Substrate discriminationUniRule annotation
Metal bindingi103 – 1031MagnesiumUniRule annotation
Active sitei104 – 1041UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Mutator protein, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repair, DNA replication

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciAHYD380703:GH2M-1145-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase IVUniRule annotation (EC:2.7.7.7UniRule annotation)
Short name:
Pol IVUniRule annotation
Gene namesi
Name:dinBUniRule annotation
Ordered Locus Names:AHA_1145
OrganismiAeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240)
Taxonomic identifieri380703 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeAeromonas
Proteomesi
  • UP000000756 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 350350DNA polymerase IVPRO_1000084876Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi380703.AHA_1145.

Structurei

3D structure databases

ProteinModelPortaliA0KHD5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 185182UmuCUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the DNA polymerase type-Y family.UniRule annotation
Contains 1 umuC domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CQ3. Bacteria.
COG0389. LUCA.
HOGENOMiHOG000082707.
KOiK02346.
OMAiWIAQEIR.
OrthoDBiEOG6FZ4D8.

Family and domain databases

Gene3Di3.30.1490.100. 1 hit.
HAMAPiMF_01113. DNApol_IV.
InterProiIPR017961. DNA_pol_Y-fam_little_finger.
IPR022880. DNApol_IV.
IPR024728. PolY_HhH_motif.
IPR001126. UmuC.
[Graphical view]
PfamiPF00817. IMS. 1 hit.
PF11799. IMS_C. 1 hit.
PF11798. IMS_HHH. 1 hit.
[Graphical view]
SUPFAMiSSF100879. SSF100879. 1 hit.
PROSITEiPS50173. UMUC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0KHD5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKIIHIDMD CFYAAVEMRD NPALREVPLA IGGSADRRGV ISTCNYVARR
60 70 80 90 100
FGVRSAMPSA LARKLCPPLV LIPGRMAVYK EVSRLLRAIF LRYTEQVEPL
110 120 130 140 150
SLDEAYLDVT MSPCCGGSAT LMAREIRAAI NTELGLTASA GVAPNKFLAK
160 170 180 190 200
LASEQRKPDG LFVIRPQEVD EFVRQLPLGK LPGIGRKTAD RLESLGLYSC
210 220 230 240 250
EDARQLGNEE LIARFGKLGE MLAGRIWGHD EQPVQAQRVR KTIGVETTLA
260 270 280 290 300
SDVLDEAACW QVLRQLIPEL EQRFSVSHGK EQLMGQGIKL KFADFQQTTV
310 320 330 340 350
YRRGGYQPER FHDLLHEGLL RAQGKPIRLL GLVVGLPAAG EVNQLALDLA
Length:350
Mass (Da):38,731
Last modified:December 12, 2006 - v1
Checksum:iF0C72E21FA239632
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000462 Genomic DNA. Translation: ABK37084.1.
RefSeqiWP_011705068.1. NC_008570.1.
YP_855686.1. NC_008570.1.

Genome annotation databases

EnsemblBacteriaiABK37084; ABK37084; AHA_1145.
GeneIDi4489943.
KEGGiaha:AHA_1145.
PATRICi20779840. VBIAerHyd135212_1150.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000462 Genomic DNA. Translation: ABK37084.1.
RefSeqiWP_011705068.1. NC_008570.1.
YP_855686.1. NC_008570.1.

3D structure databases

ProteinModelPortaliA0KHD5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi380703.AHA_1145.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK37084; ABK37084; AHA_1145.
GeneIDi4489943.
KEGGiaha:AHA_1145.
PATRICi20779840. VBIAerHyd135212_1150.

Phylogenomic databases

eggNOGiENOG4105CQ3. Bacteria.
COG0389. LUCA.
HOGENOMiHOG000082707.
KOiK02346.
OMAiWIAQEIR.
OrthoDBiEOG6FZ4D8.

Enzyme and pathway databases

BioCyciAHYD380703:GH2M-1145-MONOMER.

Family and domain databases

Gene3Di3.30.1490.100. 1 hit.
HAMAPiMF_01113. DNApol_IV.
InterProiIPR017961. DNA_pol_Y-fam_little_finger.
IPR022880. DNApol_IV.
IPR024728. PolY_HhH_motif.
IPR001126. UmuC.
[Graphical view]
PfamiPF00817. IMS. 1 hit.
PF11799. IMS_C. 1 hit.
PF11798. IMS_HHH. 1 hit.
[Graphical view]
SUPFAMiSSF100879. SSF100879. 1 hit.
PROSITEiPS50173. UMUC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240.

Entry informationi

Entry nameiDPO4_AERHH
AccessioniPrimary (citable) accession number: A0KHD5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: December 12, 2006
Last modified: November 11, 2015
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.