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Protein

DNA polymerase IV

Gene

dinB

Organism
Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII.UniRule annotation

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi8MagnesiumUniRule annotation1
Sitei13Substrate discriminationUniRule annotation1
Metal bindingi103MagnesiumUniRule annotation1
Active sitei104UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, DNA-directed DNA polymerase, Mutator protein, Nucleotidyltransferase, Transferase
Biological processDNA damage, DNA repair, DNA replication
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciAHYD380703:G1G7B-1144-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase IVUniRule annotation (EC:2.7.7.7UniRule annotation)
Short name:
Pol IVUniRule annotation
Gene namesi
Name:dinBUniRule annotation
Ordered Locus Names:AHA_1145
OrganismiAeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240)
Taxonomic identifieri380703 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeAeromonas
Proteomesi
  • UP000000756 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000848761 – 350DNA polymerase IVAdd BLAST350

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi380703.AHA_1145

Structurei

3D structure databases

ProteinModelPortaliA0KHD5
SMRiA0KHD5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 185UmuCUniRule annotationAdd BLAST182

Sequence similaritiesi

Belongs to the DNA polymerase type-Y family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CQ3 Bacteria
COG0389 LUCA
HOGENOMiHOG000082707
KOiK02346
OMAiGMQIREI

Family and domain databases

CDDicd03586 PolY_Pol_IV_kappa, 1 hit
Gene3Di3.30.1490.100, 1 hit
HAMAPiMF_01113 DNApol_IV, 1 hit
InterProiView protein in InterPro
IPR036775 DNA_pol_Y-fam_lit_finger_sf
IPR017961 DNA_pol_Y-fam_little_finger
IPR022880 DNApol_IV
IPR024728 PolY_HhH_motif
IPR001126 UmuC
PfamiView protein in Pfam
PF00817 IMS, 1 hit
PF11799 IMS_C, 1 hit
PF11798 IMS_HHH, 1 hit
SUPFAMiSSF100879 SSF100879, 1 hit
PROSITEiView protein in PROSITE
PS50173 UMUC, 1 hit

Sequencei

Sequence statusi: Complete.

A0KHD5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKIIHIDMD CFYAAVEMRD NPALREVPLA IGGSADRRGV ISTCNYVARR
60 70 80 90 100
FGVRSAMPSA LARKLCPPLV LIPGRMAVYK EVSRLLRAIF LRYTEQVEPL
110 120 130 140 150
SLDEAYLDVT MSPCCGGSAT LMAREIRAAI NTELGLTASA GVAPNKFLAK
160 170 180 190 200
LASEQRKPDG LFVIRPQEVD EFVRQLPLGK LPGIGRKTAD RLESLGLYSC
210 220 230 240 250
EDARQLGNEE LIARFGKLGE MLAGRIWGHD EQPVQAQRVR KTIGVETTLA
260 270 280 290 300
SDVLDEAACW QVLRQLIPEL EQRFSVSHGK EQLMGQGIKL KFADFQQTTV
310 320 330 340 350
YRRGGYQPER FHDLLHEGLL RAQGKPIRLL GLVVGLPAAG EVNQLALDLA
Length:350
Mass (Da):38,731
Last modified:December 12, 2006 - v1
Checksum:iF0C72E21FA239632
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000462 Genomic DNA Translation: ABK37084.1
RefSeqiWP_011705068.1, NC_008570.1
YP_855686.1, NC_008570.1

Genome annotation databases

EnsemblBacteriaiABK37084; ABK37084; AHA_1145
GeneIDi4489943
KEGGiaha:AHA_1145
PATRICifig|380703.7.peg.1150

Similar proteinsi

Entry informationi

Entry nameiDPO4_AERHH
AccessioniPrimary (citable) accession number: A0KHD5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: December 12, 2006
Last modified: March 28, 2018
This is version 68 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health