Reviewed, UniProtKB/Swiss-Prot A0KHC4 (RLMG_AERHH)

Names and origin

Protein names

Recommended name:
    Ribosomal RNA large subunit methyltransferase G
    EC=2.1.1.52
Alternative name(s):
    rRNA (guanine-N(2)-)-methyltransferase rlmG
    23S rRNA m2G1835 methyltransferase

Gene names

Name:	rlmG
Ordered Locus Names:	AHA_1134

Organism

Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) [Complete proteome] [HAMAP]

Taxonomic identifier

380703 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	380 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine + rRNA containing N(2)-methylguanine. HAMAP MF_01859
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmG family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA methylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	rRNA (guanine-N2-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 380	380	Ribosomal RNA large subunit methyltransferase G HAMAP MF_01859		PRO_0000366444

Sequences

	Sequence		Length	Mass (Da)
	A0KHC4-1 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 70F781250EFBD33D	FASTA	380	41,762
	10 20 30 40 50 60 MQTLLDTAHC RLTLYRYPRQ LQDPLQAWDA ADEYLINTLA EEPLERDGPV IIMNDGFGAL 70 80 90 100 110 120 AAYLHGHAPV SVTDSHISEQ ATLANLAENG LDPHAIRLQD ALASLPDAPA LVVIKVSKYQ 130 140 150 160 170 180 ALLEQQLLAL RQVATPATRV IAAGKAKDIH SSTLALFEKY LGPTRTSLAW KKARLIHCTP 190 200 210 220 230 240 VATPTERPAL ANPFPTVWPL EGTDMLIHNH ANVFSRTSLD IGARFMLDNL PIHSARKVID 250 260 270 280 290 300 LGCGNGVLGL SLLARDAEVE VTFIDESHMA VASARLNVEH NLPAALPRTH FLVNNCLDGV 310 320 330 340 350 360 AVGSVDRILC NPPFHQLQAI TDHIAWQMFS DAHRVLPKGG ELWIVGNRHL DYHNKLKRLF 370 380 ANAQVVASNS KFVILKAIKR

References

[1]

"Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades." Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F. J. Bacteriol. 188:8272-8282(2006) [PubMed: 16980456] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000462 Genomic DNA. Translation: ABK36108.1.
RefSeq	YP_855675.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A0KHC4.
Genome annotation databases
GeneID	4489932.
GenomeReviews	Gene locus AHA_1134 in contig CP000462_GR.
KEGG	aha:AHA_1134.
TIGR	AHA_1134.
Phylogenomic databases
OMA	NRHLGYH.
Family and domain databases
HAMAP	MF_01859. [Tree]
InterPro	IPR017237. rRNA_m2G-MeTrfase_RsmD. IPR007848. Small_mtfrase. [Graphical view]
Pfam	PF05175. MTS. 1 hit. [Graphical view]
PIRSF	PIRSF037565. RRNA_m2G_Mtase_RsmD_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMG_AERHH

Accession

Primary (citable) accession number: A0KHC4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	December 12, 2006
Last modified:	November 3, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)