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Protein

Ribosomal RNA small subunit methyltransferase A

Gene

rsmA

Organism
Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits.UniRule annotation

Catalytic activityi

4 S-adenosyl-L-methionine + adenine(1518)/adenine(1519) in 16S rRNA = 4 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(1518)/N(6)-dimethyladenine(1519) in 16S rRNA.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei19S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei21S-adenosyl-L-methionine; via amide nitrogenUniRule annotation1
Binding sitei46S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei67S-adenosyl-L-methionineUniRule annotation1
Binding sitei92S-adenosyl-L-methionineUniRule annotation1
Binding sitei114S-adenosyl-L-methionineUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase AUniRule annotation (EC:2.1.1.182UniRule annotation)
Alternative name(s):
16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferaseUniRule annotation
16S rRNA dimethyladenosine transferaseUniRule annotation
16S rRNA dimethylaseUniRule annotation
S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferaseUniRule annotation
Gene namesi
Name:rsmAUniRule annotation
Synonyms:ksgAUniRule annotation
Ordered Locus Names:AHA_0942
OrganismiAeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240)
Taxonomic identifieri380703 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeAeromonas
Proteomesi
  • UP000000756 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000565921 – 271Ribosomal RNA small subunit methyltransferase AAdd BLAST271

Interactioni

Protein-protein interaction databases

STRINGi380703.AHA_0942.

Structurei

3D structure databases

ProteinModelPortaliA0KGT8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D1X. Bacteria.
COG0030. LUCA.
HOGENOMiHOG000227962.
KOiK02528.
OMAiYKDLIPC.

Family and domain databases

Gene3Di1.10.8.100. 1 hit.
3.40.50.150. 1 hit.
HAMAPiMF_00607. 16SrRNA_methyltr_A. 1 hit.
InterProiIPR001737. KsgA/Erm.
IPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11727. PTHR11727. 1 hit.
PfamiPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTiSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00755. ksgA. 1 hit.
PROSITEiPS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0KGT8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQSNKVHMGH TARKRFGQNF LHDRYVIDQI VAAINPQPGQ NLVEIGPGLA
60 70 80 90 100
ALTEPVASQM DKMTVVELDR DLAARLREHP TLKEKLTVIE ADAMRFDFGT
110 120 130 140 150
LMGEGKGPLR IFGNLPYNIS TPLIFHLCEF ADRVEDMHFM LQKEVVLRLA
160 170 180 190 200
AGPGSKAYGR LSVMTQYYCQ VVPVLEVGPG AFKPAPKVDS AVVRLIPHKN
210 220 230 240 250
PTIVAKDIRC LNRVCTEGFG QRRKTIRNSF SNFITDAQLT ELGIDGNLRP
260 270
ENLSLEQFVM IANWLADQQQ A
Length:271
Mass (Da):30,282
Last modified:December 12, 2006 - v1
Checksum:i16698FB49B043086
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000462 Genomic DNA. Translation: ABK36812.1.
RefSeqiWP_011704879.1. NC_008570.1.
YP_855484.1. NC_008570.1.

Genome annotation databases

EnsemblBacteriaiABK36812; ABK36812; AHA_0942.
GeneIDi4486905.
KEGGiaha:AHA_0942.
PATRICi20779418. VBIAerHyd135212_0944.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000462 Genomic DNA. Translation: ABK36812.1.
RefSeqiWP_011704879.1. NC_008570.1.
YP_855484.1. NC_008570.1.

3D structure databases

ProteinModelPortaliA0KGT8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi380703.AHA_0942.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK36812; ABK36812; AHA_0942.
GeneIDi4486905.
KEGGiaha:AHA_0942.
PATRICi20779418. VBIAerHyd135212_0944.

Phylogenomic databases

eggNOGiENOG4105D1X. Bacteria.
COG0030. LUCA.
HOGENOMiHOG000227962.
KOiK02528.
OMAiYKDLIPC.

Family and domain databases

Gene3Di1.10.8.100. 1 hit.
3.40.50.150. 1 hit.
HAMAPiMF_00607. 16SrRNA_methyltr_A. 1 hit.
InterProiIPR001737. KsgA/Erm.
IPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11727. PTHR11727. 1 hit.
PfamiPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTiSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00755. ksgA. 1 hit.
PROSITEiPS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRSMA_AERHH
AccessioniPrimary (citable) accession number: A0KGT8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: December 12, 2006
Last modified: November 2, 2016
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.