A0KGJ7 (A0KGJ7_AERHH) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Non-canonical purine NTP pyrophosphatase HAMAP MF_01405
EC=3.6.1.19 HAMAP MF_01405

Alternative name(s):
Non-standard purine NTP pyrophosphatase HAMAP MF_01405
Nucleoside-triphosphate diphosphatase HAMAP MF_01405
Nucleoside-triphosphate pyrophosphatase HAMAP MF_01405

Gene names

Name:	rdgB EMBL ABK38718.1
Ordered Locus Names:	AHA_0846

Organism

Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) [Complete proteome] [HAMAP]

Taxonomic identifier

380703 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	198 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions By similarity. HAMAP MF_01405
Catalytic activity	A nucleoside triphosphate + H2O = a nucleotide + diphosphate. HAMAP MF_01405 SAAS SAAS020922
Cofactor	Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity. HAMAP MF_01405 SAAS SAAS020922
Subunit structure	Homodimer By similarity. HAMAP MF_01405 SAAS SAAS020922
Sequence similarities	Belongs to the HAM1 NTPase family. HAMAP MF_01405 RuleBase RU003781

Ontologies

Keywords
Biological process	Nucleotide metabolism HAMAP MF_01405 SAAS SAAS020922
Ligand	Magnesium HAMAP MF_01405 SAAS SAAS020922 Manganese HAMAP MF_01405 SAAS SAAS020922 Metal-binding HAMAP MF_01405 SAAS SAAS020922 Nucleotide-binding HAMAP MF_01405 SAAS SAAS020922
Molecular function	Hydrolase HAMAP MF_01405 SAAS SAAS020922 RuleBase RU003781
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphate diphosphatase activity Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	8 – 13	6	Substrate binding By similarity HAMAP MF_01405
	Region	69 – 70	2	Substrate binding By similarity HAMAP MF_01405
Sites
	Metal binding	40	1	Magnesium or manganese By similarity HAMAP MF_01405
	Metal binding	69	1	Magnesium or manganese By similarity HAMAP MF_01405
	Binding site	157	1	Substrate By similarity HAMAP MF_01405
	Binding site	177	1	Substrate By similarity HAMAP MF_01405
	Binding site	183	1	Substrate By similarity HAMAP MF_01405

Sequences

	Sequence		Length	Mass (Da)
	A0KGJ7 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 940BD56C823B3903	FASTA	198	21,474
	10 20 30 40 50 60 MNKLVLATGN QKKVKELAAM LADMKIQVIP QSEFAVSDAE ETGTTFVENA IIKARHAARI 70 80 90 100 110 120 TGLPAVADDS GLEVDLLHGR PGVYSARFAG EGASDKDNID KLLGELKGAP EYLKSARFWC 130 140 150 160 170 180 VLVYMRHADD PTPIICQASW EGMIIDEPRG QHGFGYDPVF FVPDHDCTSA QMPAELKNQL 190 SHRGQALAKL KAALAAVN

References

[1]

"Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades." Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F. J. Bacteriol. 188:8272-8282(2006) [PubMed: 16980456] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000462 Genomic DNA. Translation: ABK38718.1.
RefSeq	YP_855388.1. NC_008570.1.
3D structure databases
ProteinModelPortal	A0KGJ7.
SMR	A0KGJ7. Positions 1-195.
ModBase	Search...
Protein-protein interaction databases
STRING	A0KGJ7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4487133.
GenomeReviews	Gene locus AHA_0846 in contig CP000462_GR.
KEGG	aha:AHA_0846.
PATRIC	20779216. VBIAerHyd135212_0847.
TIGR	AHA_0846.
Phylogenomic databases
eggNOG	COG0127.
HOGENOM	HBG697237.
OMA	YSKRYDQ.
PhylomeDB	A0KGJ7.
ProtClustDB	CLSK729831.
Family and domain databases
HAMAP	MF_01405. Non_canon_purine_NTPase. [Tree]
InterPro	IPR002637. Ham1p-like. IPR020922. Nucleoside-triphosphatase. [Graphical view]
KO	K02428.
PANTHER	PTHR11067. Ham1p_like. 1 hit.
Pfam	PF01725. Ham1p_like. 1 hit. [Graphical view]
TIGRFAMs	TIGR00042. TIGR00042. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A0KGJ7_AERHH

Accession

Primary (citable) accession number: A0KGJ7

Entry history

Integrated into UniProtKB/TrEMBL:	December 12, 2006
Last sequence update:	December 12, 2006
Last modified:	December 14, 2011
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)