Reviewed, UniProtKB/Swiss-Prot A0KGJ2 (PUR9_AERHH)

Names and origin

Protein names

Recommended name:
    Bifunctional purine biosynthesis protein purH
Including the following 2 domains:
    1- Recommended name:
            Phosphoribosylaminoimidazolecarboxamide formyltransferase
              EC=2.1.2.3
        Alternative name(s):
            AICAR transformylase
    2- Recommended name:
            IMP cyclohydrolase
              EC=3.5.4.10
        Alternative name(s):
            Inosinicase
            IMP synthetase
            ATIC

Gene names

Name:	purH
Ordered Locus Names:	AHA_0841

Organism

Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) [Complete proteome] [HAMAP]

Taxonomic identifier

380703 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	530 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP MF_00139 IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP MF_00139
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1. HAMAP MF_00139 Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.
Domain	The IMP cyclohydrolase activity resides in the N-terminal region By similarity. HAMAP MF_00139
Sequence similarities	Belongs to the purH family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Hydrolase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	IMP cyclohydrolase activity Inferred from electronic annotation. Source: HAMAP phosphoribosylaminoimidazolecarboxamide formyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 530	530	Bifunctional purine biosynthesis protein purH HAMAP MF_00139		PRO_1000018832

Sequences

	Sequence		Length	Mass (Da)
	A0KGJ2-1 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 9EA6E3286CCD0888	FASTA	530	56,898
	10 20 30 40 50 60 MEQARPIRRA LLSVSDKTGI LEFAKALNER GVALLSTGGT AKLLADAGLP VTEVSDYTGF 70 80 90 100 110 120 PEMMDGRVKT LHPKVHGGIL GRREQDDAIM AQHAISPIDM VVVNLYPFAA TVAKPGCTLA 130 140 150 160 170 180 DAVENIDIGG PTMVRSAAKN HKDVAIVVKA ADYDRVIREM DGNGNSLKLA TRFDLAIAAF 190 200 210 220 230 240 EHTAAYDGMI ANYFGTMVPS YGDNKEGDEE SRFPRTFNSQ FIKKQDMRYG ENSHQAAAFY 250 260 270 280 290 300 AEEQAAPGSV ASAIQLQGKA LSYNNIADTD AALECVKEFD QPACVIVKHA NPCGVAIGSD 310 320 330 340 350 360 LLSAYERAWQ TDPTSAFGGI IAFNRELDGA TAKAIVERQF VEVIIAPTVS QAARDAVAAK 370 380 390 400 410 420 QNVRLLECGQ WTAPAQGFDF KRVNGGLLVQ ERDLGMVTLG DLTVVSKRQP TEAELNDLLF 430 440 450 460 470 480 CWKVAKFVKS NAIVYAKDGQ TIGVGAGQMS RVYSAKIAGI KAEDEGLTVA GSVMASDAFF 490 500 510 520 530 PFRDGIDAAA AAGISCVIQP GGSMRDQEVI DAADEHGMCM LFTNMRHFRH

References

[1]

"Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades." Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F. J. Bacteriol. 188:8272-8282(2006) [PubMed: 16980456] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000462 Genomic DNA. Translation: ABK36339.1.
RefSeq	YP_855383.1.
3D structure databases
SMR	A0KGJ2. Positions 7-530.
ModBase	Search...
Protein-protein interaction databases
STRING	A0KGJ2.
Genome annotation databases
GeneID	4487128.
GenomeReviews	Gene locus AHA_0841 in contig CP000462_GR.
KEGG	aha:AHA_0841.
TIGR	AHA_0841.
Phylogenomic databases
eggNOG	COG0138.
HOGENOM	HBG498048.
OMA	VVKHVKS.
PhylomeDB	A0KGJ2.
Family and domain databases
HAMAP	MF_00139. PurH. [Tree]
InterPro	IPR002695. AICARFT_IMPCHas. IPR013982. AICARFT_IMPCHase_bienz. IPR011607. MGS. [Graphical view]
PANTHER	PTHR11692. AICARFT_IMPCHas. 1 hit.
Pfam	PF01808. AICARFT_IMPCHas. 1 hit. PF02142. MGS. 1 hit. [Graphical view]
PIRSF	PIRSF000414. AICARFT_IMPCHas. 1 hit.
SMART	SM00798. AICARFT_IMPCHas. 1 hit. SM00851. MGS. 1 hit. [Graphical view]
TIGRFAMs	TIGR00355. purH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR9_AERHH

Accession

Primary (citable) accession number: A0KGJ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	December 12, 2006
Last modified:	February 9, 2010
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)