Reviewed, UniProtKB/Swiss-Prot A0KGI3 (GCP_AERHH)

Names and origin

Protein names

Recommended name:
    Probable O-sialoglycoprotein endopeptidase
      Short name=Glycoprotease
    EC=3.4.24.57

Gene names

Name:	gcp
Ordered Locus Names:	AHA_0831

Organism

Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) [Complete proteome] [HAMAP]

Taxonomic identifier

380703 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	337 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolysis of O-sialoglycoproteins; cleaves 31-Arg-|-Asp-32 bond in glycophorin A. Does not cleave unglycosylated proteins, desialylated glycoproteins or glycoproteins that are only N-glycosylated. HAMAP MF_01445
Cofactor	Zinc Probable.
Sequence similarities	Belongs to the peptidase M22 family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 337	337	Probable O-sialoglycoprotein endopeptidase HAMAP MF_01445		PRO_0000303247
Sites
	Metal binding	111	1	Zinc Potential
	Metal binding	115	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	A0KGI3-1 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: C0A092077558A650	FASTA	337	36,140
	10 20 30 40 50 60 MRVLGIETSC DETGIAIFDD QKGILSHQLY SQVKLHADYG GVVPELASRD HVRKTIPLIQ 70 80 90 100 110 120 AALREAGLGK DDIDGIAYTA GPGLVGAILV GATIGRSLAM AWNKPAIAVH HMEGHLLAPM 130 140 150 160 170 180 LEERAPEFPF VALLVSGGHS MLVRVDGIGS YQLLGESIDD AAGEAFDKTA KLMGLDYPGG 190 200 210 220 230 240 PLLSRLAEKG TTGRFTFPRP MTDRPGLDMS FSGLKTFAAN TIAANGDDEQ TRADIARAFE 250 260 270 280 290 300 DAVVDTLAIK CRRALKETGL NRLVVAGGVS ANRHLRAQLA ELMESLKGEV FYPRTEYCTD 310 320 330 NGAMIAYAGM QRLKAGVFEP LAVKAVPRWP LDTLDPI

References

[1]

"Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades." Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F. J. Bacteriol. 188:8272-8282(2006) [PubMed: 16980456] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000462 Genomic DNA. Translation: ABK39627.1.
RefSeq	YP_855374.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A0KGI3.
Protein family/group databases
MEROPS	M22.001.
Genome annotation databases
GeneID	4487118.
GenomeReviews	Gene locus AHA_0831 in contig CP000462_GR.
KEGG	aha:AHA_0831.
TIGR	AHA_0831.
Phylogenomic databases
OMA	CKRALKQ.
Family and domain databases
HAMAP	MF_01445. [Tree]
InterPro	IPR009180. Pept_M22_Osialgl. IPR000905. Peptidase_M22. IPR017860. Peptidase_M22_CS. IPR017861. Peptidase_M22_subgr. [Graphical view]
PANTHER	PTHR11735. Pept_M22_Osialgl. 1 hit.
Pfam	PF00814. Peptidase_M22. 1 hit. [Graphical view]
PRINTS	PR00789. OSIALOPTASE.
ProDom	PD002367. Peptidase_M22. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00329. gcp. 1 hit.
PROSITE	PS01016. GLYCOPROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GCP_AERHH

Accession

Primary (citable) accession number: A0KGI3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	December 12, 2006
Last modified:	November 3, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)