A0KG57 (LUXS_AERHH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
S-ribosylhomocysteine lyase
EC=4.4.1.21

Alternative name(s):
AI-2 synthesis protein
Autoinducer-2 production protein LuxS

Gene names

Name:	luxS
Ordered Locus Names:	AHA_0700

Organism

Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) [Complete proteome] [HAMAP]

Taxonomic identifier

380703 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	169 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the synthesis of autoinducer 2 (AI-2) which is secreted by bacteria and is used to communicate both the cell density and the metabolic potential of the environment. The regulation of gene expression in response to changes in cell density is called quorum sensing. Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5-dihydroxy-2,3-pentadione (DPD) By similarity. HAMAP MF_00091
Catalytic activity	S-(5-deoxy-D-ribos-5-yl)-L-homocysteine = L-homocysteine + (4S)-4,5-dihydroxypentan-2,3-dione. HAMAP MF_00091
Cofactor	Binds 1 iron ion per subunit By similarity. HAMAP MF_00091
Subunit structure	Homodimer By similarity. HAMAP MF_00091
Sequence similarities	Belongs to the LuxS family.

Ontologies

Keywords
Biological process	Autoinducer synthesis Quorum sensing
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	quorum sensing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	S-ribosylhomocysteine lyase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 169	169	S-ribosylhomocysteine lyase HAMAP MF_00091		PRO_0000297980
Sites
	Metal binding	54	1	Iron By similarity
	Metal binding	58	1	Iron By similarity
	Metal binding	128	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0KG57 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 8D134E01F27FE4DD	FASTA	169	18,777
	10 20 30 40 50 60 MPLLDSFTVD HTRMAAPAVR VAKTMQTPNK DTITVFDLRF CVPNQEILSE RGIHTLEHLF 70 80 90 100 110 120 AGFMRDHLNG NGVEIIDISP MGCRTGFYMS LIGAPDEARV GAAWQAAMSD VLTVQEQGKI 130 140 150 160 PELNEYQCGT YSMHSLEEAH AIARHVLERG IGVNRNDELA LPEEKLKSL

References

[1]

"Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades." Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F. J. Bacteriol. 188:8272-8282(2006) [PubMed: 16980456] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 7966 / NCIB 9240.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000462 Genomic DNA. Translation: ABK38869.1.
RefSeq	YP_855242.1. NC_008570.1.
3D structure databases
ProteinModelPortal	A0KG57.
SMR	A0KG57. Positions 3-161.
ModBase	Search...
Protein-protein interaction databases
STRING	A0KG57.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4488322.
GenomeReviews	Gene locus AHA_0700 in contig CP000462_GR.
KEGG	aha:AHA_0700.
PATRIC	20778914. VBIAerHyd135212_0702.
TIGR	AHA_0700.
Phylogenomic databases
eggNOG	COG1854.
HOGENOM	HBG347473.
OMA	PHGDTIT.
PhylomeDB	A0KG57.
ProtClustDB	PRK02260.
Enzyme and pathway databases
BioCyc	AHYD196024:AHA_0700-MONOMER.
Family and domain databases
HAMAP	MF_00091. LuxS. [Tree]
InterPro	IPR011249. Metalloenz_metal-bd. IPR003815. S-ribosylhomocysteinase. [Graphical view]
Gene3D	G3DSA:3.30.1360.80. S-ribosylhomocysteinase. 1 hit.
KO	K07173.
Pfam	PF02664. LuxS. 1 hit. [Graphical view]
PIRSF	PIRSF006160. AI2. 1 hit.
PRINTS	PR01487. LUXSPROTEIN.
ProDom	PD013172. S-ribosylhomocysteinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF63411. Metalloenz_metal-bd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LUXS_AERHH

Accession

Primary (citable) accession number: A0KG57

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 21, 2007
Last sequence update:	December 12, 2006
Last modified:	December 14, 2011
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program