Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei577Aminoacyl-adenylateUniRule annotation1
Binding sitei621ATPUniRule annotation1
Metal bindingi916ZincUniRule annotation1
Metal bindingi919ZincUniRule annotation1
Metal bindingi936ZincUniRule annotation1
Metal bindingi939ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciAHYD380703:G1G7B-683-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:AHA_0682
OrganismiAeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240)
Taxonomic identifieri380703 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeAeromonas
Proteomesi
  • UP000000756 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000220371 – 953Isoleucine--tRNA ligaseAdd BLAST953

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi380703.AHA_0682

Structurei

3D structure databases

ProteinModelPortaliA0KG39
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi58 – 68"HIGH" regionAdd BLAST11
Motifi618 – 622"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A0KG39-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDYKHTLNL PETEFPMRGD LAKREPNMLK RWYDQDLYGA IRRAKAGKPS
60 70 80 90 100
FILHDGPPYA NGSIHIGHSV NKILKDIIIK SKGLSGFDSP YVPGWDCHGL
110 120 130 140 150
PIELKVEGMV GKPGEKVSAA EFRAECRKYA KTQIEAQKTD FIRLGVLGDW
160 170 180 190 200
EHPYLTMDFG TEANIIRSMA KIVENGHLHK GSKPVHWCTD CGSALAEAEV
210 220 230 240 250
EYYDKNSPSI DVRFKAVDEA GVAAKFDCAE GHTGKGPISA VIWTTTPWTL
260 270 280 290 300
PANRGIAMHA DLDYALVQVE GEHPERLILA AELVKDVMDR AGIEQFHNLG
310 320 330 340 350
YAKGAALELL RFNHPFYSFD VPVVLGDHVT LDAGTGAVHT APGHGQEDFV
360 370 380 390 400
VGQKYGLEVA NPVGSNGVYL PDTELFAGQH VFKANASVVE VLTERGALLH
410 420 430 440 450
HKVFNHSYPH CWRHKTPIIF RATPQWFISM EQKGLRQRAL EEIERIEQDG
460 470 480 490 500
IKQHGQSGWV PAWGKNRIQA MVENRPDWCI SRQRTWGVPI SLFVHKETQE
510 520 530 540 550
LHPESVRLMH EVAKRVEQSG IQAWWDLDKA ELLGSDADLY DKVPDTLDVW
560 570 580 590 600
FDSGSTHSSV VDARPEFNGN PADMYLEGSD QHRGWFMSSL MIGVAMKDKA
610 620 630 640 650
PYNQVLTHGF TVDGQGRKMS KSIGNVVSPQ DVMNKLGADI LRLWVASTDY
660 670 680 690 700
TGEMTVSDEI LKRSADAYRR IRNTARFLLA NLNGFNPATD MVAPADMVVV
710 720 730 740 750
DRWAVGRAKA VQAEIMAAFD EYNFHGVTQK LMQFCSIEMG SFYLDVIKDR
760 770 780 790 800
QYTAKADSLA RRSCQTALYH IAEAMVRWMA PIMSFTADEI WALLPGERGE
810 820 830 840 850
FVFTEEWYDG LFGLEAGETL NDDFWAEILT VRGEVNKALE VARGEKRIGG
860 870 880 890 900
SLQAELTLFA KPALAARLNA LADELRFVLL TSKAKVVSVD AAPADAVATE
910 920 930 940 950
RDDLWLSVAQ SAAAKCDRCW HHVEDVGTIA GHEEICGRCV TNVEGDGETR

QFA
Length:953
Mass (Da):106,106
Last modified:December 12, 2006 - v1
Checksum:iB14EE34C3A6A5265
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000462 Genomic DNA Translation: ABK37838.1
RefSeqiWP_011704643.1, NC_008570.1
YP_855224.1, NC_008570.1

Genome annotation databases

EnsemblBacteriaiABK37838; ABK37838; AHA_0682
GeneIDi4487674
KEGGiaha:AHA_0682
PATRICifig|380703.7.peg.684

Similar proteinsi

Entry informationi

Entry nameiSYI_AERHH
AccessioniPrimary (citable) accession number: A0KG39
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: December 12, 2006
Last modified: March 28, 2018
This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health