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A0KG39

- SYI_AERHH

UniProt

A0KG39 - SYI_AERHH

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei577 – 5771Aminoacyl-adenylateUniRule annotation
Binding sitei621 – 6211ATPUniRule annotation
Metal bindingi916 – 9161ZincUniRule annotation
Metal bindingi919 – 9191ZincUniRule annotation
Metal bindingi936 – 9361ZincUniRule annotation
Metal bindingi939 – 9391ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciAHYD380703:GH2M-683-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:AHA_0682
OrganismiAeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240)
Taxonomic identifieri380703 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeAeromonas
ProteomesiUP000000756: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 953953Isoleucine--tRNA ligasePRO_1000022037Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi380703.AHA_0682.

Structurei

3D structure databases

ProteinModelPortaliA0KG39.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi618 – 6225"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0KG39-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDYKHTLNL PETEFPMRGD LAKREPNMLK RWYDQDLYGA IRRAKAGKPS
60 70 80 90 100
FILHDGPPYA NGSIHIGHSV NKILKDIIIK SKGLSGFDSP YVPGWDCHGL
110 120 130 140 150
PIELKVEGMV GKPGEKVSAA EFRAECRKYA KTQIEAQKTD FIRLGVLGDW
160 170 180 190 200
EHPYLTMDFG TEANIIRSMA KIVENGHLHK GSKPVHWCTD CGSALAEAEV
210 220 230 240 250
EYYDKNSPSI DVRFKAVDEA GVAAKFDCAE GHTGKGPISA VIWTTTPWTL
260 270 280 290 300
PANRGIAMHA DLDYALVQVE GEHPERLILA AELVKDVMDR AGIEQFHNLG
310 320 330 340 350
YAKGAALELL RFNHPFYSFD VPVVLGDHVT LDAGTGAVHT APGHGQEDFV
360 370 380 390 400
VGQKYGLEVA NPVGSNGVYL PDTELFAGQH VFKANASVVE VLTERGALLH
410 420 430 440 450
HKVFNHSYPH CWRHKTPIIF RATPQWFISM EQKGLRQRAL EEIERIEQDG
460 470 480 490 500
IKQHGQSGWV PAWGKNRIQA MVENRPDWCI SRQRTWGVPI SLFVHKETQE
510 520 530 540 550
LHPESVRLMH EVAKRVEQSG IQAWWDLDKA ELLGSDADLY DKVPDTLDVW
560 570 580 590 600
FDSGSTHSSV VDARPEFNGN PADMYLEGSD QHRGWFMSSL MIGVAMKDKA
610 620 630 640 650
PYNQVLTHGF TVDGQGRKMS KSIGNVVSPQ DVMNKLGADI LRLWVASTDY
660 670 680 690 700
TGEMTVSDEI LKRSADAYRR IRNTARFLLA NLNGFNPATD MVAPADMVVV
710 720 730 740 750
DRWAVGRAKA VQAEIMAAFD EYNFHGVTQK LMQFCSIEMG SFYLDVIKDR
760 770 780 790 800
QYTAKADSLA RRSCQTALYH IAEAMVRWMA PIMSFTADEI WALLPGERGE
810 820 830 840 850
FVFTEEWYDG LFGLEAGETL NDDFWAEILT VRGEVNKALE VARGEKRIGG
860 870 880 890 900
SLQAELTLFA KPALAARLNA LADELRFVLL TSKAKVVSVD AAPADAVATE
910 920 930 940 950
RDDLWLSVAQ SAAAKCDRCW HHVEDVGTIA GHEEICGRCV TNVEGDGETR

QFA
Length:953
Mass (Da):106,106
Last modified:December 12, 2006 - v1
Checksum:iB14EE34C3A6A5265
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000462 Genomic DNA. Translation: ABK37838.1.
RefSeqiYP_855224.1. NC_008570.1.

Genome annotation databases

EnsemblBacteriaiABK37838; ABK37838; AHA_0682.
GeneIDi4487674.
KEGGiaha:AHA_0682.
PATRICi20778878. VBIAerHyd135212_0684.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000462 Genomic DNA. Translation: ABK37838.1 .
RefSeqi YP_855224.1. NC_008570.1.

3D structure databases

ProteinModelPortali A0KG39.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 380703.AHA_0682.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABK37838 ; ABK37838 ; AHA_0682 .
GeneIDi 4487674.
KEGGi aha:AHA_0682.
PATRICi 20778878. VBIAerHyd135212_0684.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci AHYD380703:GH2M-683-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 7966 / NCIB 9240.

Entry informationi

Entry nameiSYI_AERHH
AccessioniPrimary (citable) accession number: A0KG39
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: December 12, 2006
Last modified: October 29, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3