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Protein

tRNA (guanine-N(1)-)-methyltransferase

Gene

trmD

Organism
Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Specifically methylates guanosine-37 in various tRNAs.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei113 – 1131S-adenosyl-L-methionine; via amide nitrogenUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciAHYD380703:GH2M-669-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(1)-)-methyltransferaseUniRule annotation (EC:2.1.1.228UniRule annotation)
Alternative name(s):
M1G-methyltransferaseUniRule annotation
tRNA [GM37] methyltransferaseUniRule annotation
Gene namesi
Name:trmDUniRule annotation
Ordered Locus Names:AHA_0668
OrganismiAeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240)
Taxonomic identifieri380703 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeAeromonas
Proteomesi
  • UP000000756 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 249249tRNA (guanine-N(1)-)-methyltransferasePRO_1000006444Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi380703.AHA_0668.

Structurei

3D structure databases

ProteinModelPortaliA0KG25.
SMRiA0KG25. Positions 1-239.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni133 – 1386S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the RNA methyltransferase TrmD family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D6X. Bacteria.
COG0336. LUCA.
HOGENOMiHOG000016242.
KOiK00554.
OMAiYKGVDQR.

Family and domain databases

Gene3Di1.10.1270.20. 1 hit.
3.40.1280.10. 1 hit.
HAMAPiMF_00605. TrmD. 1 hit.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR002649. tRNA_m1G_MeTrfase_bac.
IPR023148. tRNA_m1G_MeTrfase_C.
IPR029026. tRNA_m1G_MTases_N.
IPR016009. tRNA_MeTrfase_TRMD/TRM10.
[Graphical view]
PfamiPF01746. tRNA_m1G_MT. 1 hit.
[Graphical view]
PIRSFiPIRSF000386. tRNA_mtase. 1 hit.
SUPFAMiSSF75217. SSF75217. 1 hit.
TIGRFAMsiTIGR00088. trmD. 1 hit.

Sequencei

Sequence statusi: Complete.

A0KG25-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWIGVISLFP EMFRAITEHG VTGRAVKSGL LQIECWNPRD FTHDKHRTVD
60 70 80 90 100
DRPYGGGPGM LMMVQPLRDA IHAAKQAAGD GAKVIYLSPQ GRKLTQAGVT
110 120 130 140 150
ELATNQKLIL VAGRYEGIDE RVIQTEVDEE WSIGDYVLSG GELPAMTLID
160 170 180 190 200
AVSRLVPGVL GDQASAEQDS FTDGLLDHPH YTRPELLDGL AVPEALTSGN
210 220 230 240
HEVIRRWRLK QSLGRTWQRR PELINNLALT DEQESLLAEY VREVRDSVK
Length:249
Mass (Da):27,689
Last modified:December 12, 2006 - v1
Checksum:i5812610BB4FE13A9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000462 Genomic DNA. Translation: ABK39410.1.
RefSeqiWP_011704631.1. NC_008570.1.
YP_855210.1. NC_008570.1.

Genome annotation databases

EnsemblBacteriaiABK39410; ABK39410; AHA_0668.
GeneIDi4490675.
KEGGiaha:AHA_0668.
PATRICi20778848. VBIAerHyd135212_0669.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000462 Genomic DNA. Translation: ABK39410.1.
RefSeqiWP_011704631.1. NC_008570.1.
YP_855210.1. NC_008570.1.

3D structure databases

ProteinModelPortaliA0KG25.
SMRiA0KG25. Positions 1-239.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi380703.AHA_0668.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK39410; ABK39410; AHA_0668.
GeneIDi4490675.
KEGGiaha:AHA_0668.
PATRICi20778848. VBIAerHyd135212_0669.

Phylogenomic databases

eggNOGiENOG4105D6X. Bacteria.
COG0336. LUCA.
HOGENOMiHOG000016242.
KOiK00554.
OMAiYKGVDQR.

Enzyme and pathway databases

BioCyciAHYD380703:GH2M-669-MONOMER.

Family and domain databases

Gene3Di1.10.1270.20. 1 hit.
3.40.1280.10. 1 hit.
HAMAPiMF_00605. TrmD. 1 hit.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR002649. tRNA_m1G_MeTrfase_bac.
IPR023148. tRNA_m1G_MeTrfase_C.
IPR029026. tRNA_m1G_MTases_N.
IPR016009. tRNA_MeTrfase_TRMD/TRM10.
[Graphical view]
PfamiPF01746. tRNA_m1G_MT. 1 hit.
[Graphical view]
PIRSFiPIRSF000386. tRNA_mtase. 1 hit.
SUPFAMiSSF75217. SSF75217. 1 hit.
TIGRFAMsiTIGR00088. trmD. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTRMD_AERHH
AccessioniPrimary (citable) accession number: A0KG25
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: December 12, 2006
Last modified: September 7, 2016
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.