ID   MDH_AERHH               Reviewed;         311 AA.
AC   A0KG16;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   12-DEC-2006, sequence version 1.
DT   16-JUN-2009, entry version 17.
DE   RecName: Full=Malate dehydrogenase;
DE            EC=1.1.1.37;
GN   Name=mdh; OrderedLocusNames=AHA_0659;
OS   Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Aeromonadales;
OC   Aeromonadaceae; Aeromonas.
OX   NCBI_TaxID=380703;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16980456; DOI=10.1128/JB.00621-06;
RA   Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J.,
RA   Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M.,
RA   Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F.;
RT   "Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all
RT   trades.";
RL   J. Bacteriol. 188:8272-8282(2006).
CC   -!- FUNCTION: Catalyzes the reversible oxidation of malate to
CC       oxaloacetate (By similarity).
CC   -!- CATALYTIC ACTIVITY: (S)-malate + NAD(+) = oxaloacetate + NADH.
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SIMILARITY: Belongs to the LDH/MDH superfamily. MDH type 1 family.
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DR   EMBL; CP000462; ABK35951.1; -; Genomic_DNA.
DR   RefSeq; YP_855201.1; -.
DR   SMR; A0KG16; 1-310.
DR   GeneID; 4486843; -.
DR   GenomeReviews; CP000462_GR; AHA_0659.
DR   KEGG; aha:AHA_0659; -.
DR   TIGR; AHA_0659; -.
DR   OMA; A0KG16; MGWTTQE.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0030060; F:L-malate dehydrogenase activity; IEA:HAMAP.
DR   GO; GO:0006096; P:glycolysis; IEA:InterPro.
DR   GO; GO:0006108; P:malate metabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:HAMAP.
DR   HAMAP; MF_01516; -; 1.
DR   InterPro; IPR001557; L-lactate/malate_DH.
DR   InterPro; IPR001236; Lactate/malate_DH.
DR   InterPro; IPR015955; Lactate_DH/Glyco_Ohase_4_C.
DR   InterPro; IPR001252; Malate_DH_AS.
DR   InterPro; IPR010097; Malate_DH_NAD-dep_euk_g_bac.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.90.110.10; lact_mal_DH; 1.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   PANTHER; PTHR11540:SF1; MDH_euk_g_bac; 1.
DR   Pfam; PF02866; Ldh_1_C; 1.
DR   Pfam; PF00056; Ldh_1_N; 1.
DR   PIRSF; PIRSF000102; Lac_mal_DH; 1.
DR   TIGRFAMs; TIGR01772; MDH_euk_gproteo; 1.
DR   PROSITE; PS00068; MDH; 1.
PE   3: Inferred from homology;
KW   Complete proteome; NAD; Oxidoreductase; Tricarboxylic acid cycle.
FT   CHAIN         1    311       Malate dehydrogenase.
FT                                /FTId=PRO_0000294292.
FT   NP_BIND       7     13       NAD (By similarity).
FT   NP_BIND     117    119       NAD (By similarity).
FT   ACT_SITE    177    177       Proton acceptor (By similarity).
FT   BINDING      34     34       NAD (By similarity).
FT   BINDING      81     81       Substrate (By similarity).
FT   BINDING      87     87       Substrate (By similarity).
FT   BINDING      94     94       NAD (By similarity).
FT   BINDING     119    119       Substrate (By similarity).
FT   BINDING     153    153       Substrate (By similarity).
FT   BINDING     227    227       NAD (By similarity).
SQ   SEQUENCE   311 AA;  32113 MW;  B7976C035279DA6E CRC64;
     MKVAVLGAAG GIGQALALLL KNRLPAGSEL SLYDIAPVTP GVAVDLSHIP TDVKVKGFCG
     EDPSPALVGA DVVLISAGVA RKPGMDRSDL FNINAGIVKN LVEKCAASCP KALIGIITNP
     VNTTVAIAAE VLKKAGVYDK RRLFGVTTLD VIRAETFVAE AKGLNVDKVR VNVIGGHSGV
     TILPLLSQIE GASFTADEVA AMTKRIQNAG TEVVEAKAGG GSATLSMGQA ACRFGLSLIK
     GLQGEANVIE CAYVEGDGKH ATFFAQPILL GKNGVETVLD YGKLSAFEQE AMDGMLATLK
     ADIQLGVEFV K
//