A0KG16 (MDH_AERHH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate dehydrogenase
EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	AHA_0659

Organism

Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) [Complete proteome] [HAMAP]

Taxonomic identifier

380703 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP MF_01516
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP MF_01516
Subunit structure	Homodimer By similarity. HAMAP MF_01516
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 1 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 311	311	Malate dehydrogenase HAMAP MF_01516		PRO_0000294292
Regions
	Nucleotide binding	7 – 13	7	NAD By similarity
	Nucleotide binding	117 – 119	3	NAD By similarity
Sites
	Active site	177	1	Proton acceptor By similarity
	Binding site	34	1	NAD By similarity
	Binding site	81	1	Substrate By similarity
	Binding site	87	1	Substrate By similarity
	Binding site	94	1	NAD By similarity
	Binding site	119	1	Substrate By similarity
	Binding site	153	1	Substrate By similarity
	Binding site	227	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0KG16 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: B7976C035279DA6E	FASTA	311	32,113
	10 20 30 40 50 60 MKVAVLGAAG GIGQALALLL KNRLPAGSEL SLYDIAPVTP GVAVDLSHIP TDVKVKGFCG 70 80 90 100 110 120 EDPSPALVGA DVVLISAGVA RKPGMDRSDL FNINAGIVKN LVEKCAASCP KALIGIITNP 130 140 150 160 170 180 VNTTVAIAAE VLKKAGVYDK RRLFGVTTLD VIRAETFVAE AKGLNVDKVR VNVIGGHSGV 190 200 210 220 230 240 TILPLLSQIE GASFTADEVA AMTKRIQNAG TEVVEAKAGG GSATLSMGQA ACRFGLSLIK 250 260 270 280 290 300 GLQGEANVIE CAYVEGDGKH ATFFAQPILL GKNGVETVLD YGKLSAFEQE AMDGMLATLK 310 ADIQLGVEFV K

References

[1]

"Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades." Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F. J. Bacteriol. 188:8272-8282(2006) [PubMed: 16980456] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 7966 / NCIB 9240.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000462 Genomic DNA. Translation: ABK35951.1.
RefSeq	YP_855201.1. NC_008570.1.
3D structure databases
ProteinModelPortal	A0KG16.
SMR	A0KG16. Positions 1-310.
ModBase	Search...
Protein-protein interaction databases
STRING	A0KG16.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4486843.
GenomeReviews	Gene locus AHA_0659 in contig CP000462_GR.
KEGG	aha:AHA_0659.
PATRIC	20778830. VBIAerHyd135212_0660.
TIGR	AHA_0659.
Phylogenomic databases
eggNOG	COG0039.
HOGENOM	HBG566126.
OMA	LVEKCAA.
PhylomeDB	A0KG16.
ProtClustDB	PRK05086.
Enzyme and pathway databases
BioCyc	AHYD196024:AHA_0659-MONOMER.
Family and domain databases
HAMAP	MF_01516. Malate_dehydrog_1. [Tree]
InterPro	IPR001557. L-lactate/malate_DH. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR001252. Malate_DH_AS. IPR010097. Malate_DH_type1. IPR023958. Malate_DH_type1_bac. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00024.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01772. MDH_euk_gproteo. 1 hit.
PROSITE	PS00068. MDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDH_AERHH

Accession

Primary (citable) accession number: A0KG16

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	December 12, 2006
Last modified:	December 14, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program