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Reviewed, UniProtKB/Swiss-Prot A0KFV3 (ASSY_AERHH)

Last modified November 3, 2009. Version 20. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Argininosuccinate synthase
    EC=6.3.4.5
Alternative name(s):
    Citrulline--aspartate ligase
Gene names
Name: argG
Ordered Locus Names: AHA_0596
OrganismAeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) [Complete proteome] [HAMAP]
Taxonomic identifier380703 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

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Protein attributes

Sequence length411 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Catalytic activity

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP MF_00005

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP MF_00005

Subunit structure

Homotetramer By similarity.

Subcellular location

Cytoplasm Probable.

Sequence similarities

Belongs to the argininosuccinate synthase family. Type 1 subfamily.

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Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Arginine biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processarginine biosynthetic process

Inferred from electronic annotation. Source: HAMAP

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: HAMAP

argininosuccinate synthase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 411411Argininosuccinate synthase HAMAP MF_00005
PRO_0000321297

Regions

Nucleotide binding10 – 189ATP By similarity

Sites

Binding site371ATP; via amide nitrogen and carbonyl oxygen By similarity
Binding site891Citrulline By similarity
Binding site941Citrulline By similarity
Binding site1191ATP; via amide nitrogen By similarity
Binding site1211Aspartate By similarity
Binding site1251Aspartate By similarity
Binding site1251Citrulline By similarity
Binding site1261Aspartate By similarity
Binding site1291Citrulline By similarity
Binding site1781Citrulline By similarity
Binding site1871Citrulline By similarity
Binding site2631Citrulline By similarity
Binding site2751Citrulline By similarity

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Sequences

Sequence LengthMass (Da)Tools
A0KFV3-1 [UniParc].

Last modified December 12, 2006. Version 1.
Checksum: 99C9323F9F0BFE8B

FASTA41145,190
        10         20         30         40         50         60 
MSGINKIVLA YSGGLDTSAI IPWLKENYDA EIIAFVADVG QERDDLEGIE QKAIASGATK 

        70         80         90        100        110        120 
CIVKDLREEF VKEYVYPTLK TGAVYEGTYL LGTSMARPVI AKAMVEAALA EGADAISHGC 

       130        140        150        160        170        180 
TGKGNDQVRF EGAVAALAPQ LKVIAPWRLW DMRSREDLLA YLETRNIPCK ATLKKIYSRD 

       190        200        210        220        230        240 
ANAWHISTEG GELESTWNEP SEAVWQWTVP AEQAPDQPEY VKLTVAQGEV VAVDDQPLSP 

       250        260        270        280        290        300 
HQILTTLNER AGKHGVGRID ITENRMVGMK SRGCYETPGG TVMVAALRAV EELVLDRPTR 

       310        320        330        340        350        360 
AWREKLGAEF SHLVYDGRWF TPLCKAIVAS ANAIAEDLDG EVVLKMYKGQ VTAVQKKSPN 

       370        380        390        400        410 
SLYSEDFATF GADEVYDQSH AEGFIRLYTL ASRIRAMKEQ HQAIGGDHTH G

« Hide

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References

[1]"Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades."
Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F.
J. Bacteriol. 188:8272-8282(2006) [PubMed: 16980456] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases

CP000462 Genomic DNA. Translation: ABK39577.1.
RefSeqYP_855129.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGA0KFV3.

Genome annotation databases

GeneID4489263.
GenomeReviewsGene locus AHA_0596 in contig CP000462_GR.
KEGGaha:AHA_0596.
TIGRAHA_0596.

Phylogenomic databases

OMAIAPVREW.

Family and domain databases

HAMAPMF_00005.
[Tree]
InterProIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
Gene3DG3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHERPTHR11587. Arginosuc_synth. 1 hit.
PfamPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsTIGR00032. argG. 1 hit.
PROSITEPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameASSY_AERHH
AccessionPrimary (citable) accession number: A0KFV3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: December 12, 2006
Last modified: November 3, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents