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Protein

Argininosuccinate synthase

Gene

argG

Organism
Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei37 – 371ATP; via amide nitrogen and carbonyl oxygenUniRule annotation
Binding sitei89 – 891CitrullineUniRule annotation
Binding sitei94 – 941CitrullineUniRule annotation
Binding sitei119 – 1191ATP; via amide nitrogenUniRule annotation
Binding sitei121 – 1211AspartateUniRule annotation
Binding sitei125 – 1251AspartateUniRule annotation
Binding sitei125 – 1251CitrullineUniRule annotation
Binding sitei126 – 1261AspartateUniRule annotation
Binding sitei129 – 1291CitrullineUniRule annotation
Binding sitei178 – 1781CitrullineUniRule annotation
Binding sitei187 – 1871CitrullineUniRule annotation
Binding sitei263 – 2631CitrullineUniRule annotation
Binding sitei275 – 2751CitrullineUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 189ATPUniRule annotation

GO - Molecular functioni

  1. argininosuccinate synthase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. arginine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAHYD380703:GH2M-597-MONOMER.
UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:AHA_0596
OrganismiAeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240)
Taxonomic identifieri380703 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeAeromonas
ProteomesiUP000000756: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 411411Argininosuccinate synthasePRO_0000321297Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi380703.AHA_0596.

Structurei

3D structure databases

ProteinModelPortaliA0KFV3.
SMRiA0KFV3. Positions 6-400.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
KOiK01940.
OMAiPAREWGM.
OrthoDBiEOG6K9QCV.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0KFV3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSGINKIVLA YSGGLDTSAI IPWLKENYDA EIIAFVADVG QERDDLEGIE
60 70 80 90 100
QKAIASGATK CIVKDLREEF VKEYVYPTLK TGAVYEGTYL LGTSMARPVI
110 120 130 140 150
AKAMVEAALA EGADAISHGC TGKGNDQVRF EGAVAALAPQ LKVIAPWRLW
160 170 180 190 200
DMRSREDLLA YLETRNIPCK ATLKKIYSRD ANAWHISTEG GELESTWNEP
210 220 230 240 250
SEAVWQWTVP AEQAPDQPEY VKLTVAQGEV VAVDDQPLSP HQILTTLNER
260 270 280 290 300
AGKHGVGRID ITENRMVGMK SRGCYETPGG TVMVAALRAV EELVLDRPTR
310 320 330 340 350
AWREKLGAEF SHLVYDGRWF TPLCKAIVAS ANAIAEDLDG EVVLKMYKGQ
360 370 380 390 400
VTAVQKKSPN SLYSEDFATF GADEVYDQSH AEGFIRLYTL ASRIRAMKEQ
410
HQAIGGDHTH G
Length:411
Mass (Da):45,190
Last modified:December 12, 2006 - v1
Checksum:i99C9323F9F0BFE8B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000462 Genomic DNA. Translation: ABK39577.1.
RefSeqiYP_855129.1. NC_008570.1.

Genome annotation databases

EnsemblBacteriaiABK39577; ABK39577; AHA_0596.
GeneIDi4489263.
KEGGiaha:AHA_0596.
PATRICi20778696. VBIAerHyd135212_0593.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000462 Genomic DNA. Translation: ABK39577.1.
RefSeqiYP_855129.1. NC_008570.1.

3D structure databases

ProteinModelPortaliA0KFV3.
SMRiA0KFV3. Positions 6-400.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi380703.AHA_0596.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK39577; ABK39577; AHA_0596.
GeneIDi4489263.
KEGGiaha:AHA_0596.
PATRICi20778696. VBIAerHyd135212_0593.

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
KOiK01940.
OMAiPAREWGM.
OrthoDBiEOG6K9QCV.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.
BioCyciAHYD380703:GH2M-597-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 7966 / NCIB 9240.

Entry informationi

Entry nameiASSY_AERHH
AccessioniPrimary (citable) accession number: A0KFV3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: December 12, 2006
Last modified: February 4, 2015
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.