A0KFT8 (SYY_AERHH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine--tRNA ligase
EC=6.1.1.1

Alternative name(s):
Tyrosyl-tRNA synthetase
Short name=TyrRS

Gene names

Name:	tyrS
Ordered Locus Names:	AHA_0581

Organism

Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) [Complete proteome] [HAMAP]

Taxonomic identifier

380703 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	422 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity. HAMAP MF_02006
Catalytic activity	ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr). HAMAP MF_02006
Subunit structure	Homodimer By similarity. HAMAP MF_02006
Subcellular location	Cytoplasm By similarity HAMAP MF_02006.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 1 subfamily. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tyrosyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: UniProtKB-KW tyrosine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 422	422	Tyrosine--tRNA ligase HAMAP MF_02006		PRO_1000088574
Regions
	Domain	356 – 420	65	S4 RNA-binding
	Motif	41 – 50	10	"HIGH" region HAMAP MF_02006
	Motif	234 – 238	5	"KMSKS" region HAMAP MF_02006
Sites
	Binding site	36	1	Tyrosine By similarity
	Binding site	174	1	Tyrosine By similarity
	Binding site	178	1	Tyrosine By similarity
	Binding site	237	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0KFT8 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 3BF04C85474299AB	FASTA	422	46,360
	10 20 30 40 50 60 MINPHLLDEL TRRGLVAQNS DPVALADHLA TPRTVYCGFD PTAGSLHIGH LVPLLMLRRF 70 80 90 100 110 120 QLAGHTPVAL VGGATGLIGD PSFKATERNL NSSETVQGWV ASLSAQIKAL LPASEGLAAP 130 140 150 160 170 180 LLVNNADWMG QMSALDFLRD IGKHFSVNAM LVRESVRQRL ARPDQGISFT EFSYALLQSQ 190 200 210 220 230 240 DYAVLNQRLG CTLQIGGNDQ WGNITSGMDL TRRLHQAQVY GMTLPLITKA DGTKFGKTEG 250 260 270 280 290 300 GAIWLDPALT SPYAFYQFWL GTADEDVYRF LRYYSFMPLT EIEALEAEDA KRQGRKLAQQ 310 320 330 340 350 360 VLADELTELV HGKGALAAAQ RISELLFSGE VARLGESDLA QLAQDGMPGW RIEGETDLVT 370 380 390 400 410 420 LLVESGLANS KRIARELLAA GAISLNGEIR REEPLRAADR LFGRHLLLRR GKKQYRLVTW QG

References

[1]

"Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades." Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F. J. Bacteriol. 188:8272-8282(2006) [PubMed: 16980456] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 7966 / NCIB 9240.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000462 Genomic DNA. Translation: ABK39461.1.
RefSeq	YP_855114.1. NC_008570.1.
3D structure databases
ProteinModelPortal	A0KFT8.
SMR	A0KFT8. Positions 1-321.
ModBase	Search...
Protein-protein interaction databases
STRING	A0KFT8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4489403.
GenomeReviews	Gene locus AHA_0581 in contig CP000462_GR.
KEGG	aha:AHA_0581.
PATRIC	20778664. VBIAerHyd135212_0577.
TIGR	AHA_0581.
Phylogenomic databases
eggNOG	COG0162.
HOGENOM	HBG288125.
OMA	TFYIGFD.
PhylomeDB	A0KFT8.
ProtClustDB	PRK05912.
Enzyme and pathway databases
BioCyc	AHYD196024:AHA_0581-MONOMER.
Family and domain databases
HAMAP	MF_02006. Tyr_tRNA_synth_type1. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ic. IPR014729. Rossmann-like_a/b/a_fold. IPR002942. S4_RNA-bd. IPR002307. Tyr-tRNA-synth. IPR024088. Tyr-tRNA-synth_bac-type. IPR024107. Tyr-tRNA-synth_bac_1. [Graphical view]
Gene3D	G3DSA:3.10.290.10. G3DSA:3.10.290.10. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01866.
PANTHER	PTHR11766. Tyr_tRNA-synt_1b. 1 hit.
Pfam	PF01479. S4. 1 hit. PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01040. TRNASYNTHTYR.
SMART	SM00363. S4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00234. TyrS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50889. S4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYY_AERHH

Accession

Primary (citable) accession number: A0KFT8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	December 12, 2006
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program