Reviewed, UniProtKB/Swiss-Prot A0KEX8 (AROE_AERHH)

Names and origin

Protein names

Recommended name:
    Shikimate dehydrogenase
    EC=1.1.1.25

Gene names

Name:	aroE
Ordered Locus Names:	AHA_0266

Organism

Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) [Complete proteome] [HAMAP]

Taxonomic identifier

380703 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Shikimate + NADP+ = 3-dehydroshikimate + NADPH. HAMAP MF_00222
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7. HAMAP MF_00222
Sequence similarities	Belongs to the shikimate dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro shikimate 5-dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 273	273	Shikimate dehydrogenase HAMAP MF_00222		PRO_1000021253
Regions
	Nucleotide binding	126 – 130	5	NADP By similarity
Sites
	Active site	65	1	Proton acceptor Potential

Sequences

	Sequence		Length	Mass (Da)
	A0KEX8-1 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 7D837CC96DD7FED7	FASTA	273	29,192
	10 20 30 40 50 60 MDRYLVFGHP VRHSKSPFIH TLFARQTQQE LEYGLAEPAV EEFATSLRAF FAQGGKGCNV 70 80 90 100 110 120 TVPFKEQAFA LVDRLSPRAR RAGAVNTIKL TDDGVLLGDN TDGAGLVADL KAHGVALAGS 130 140 150 160 170 180 RILLLGAGGA ARGALAPLLA EQPEALVIAN RTHARAEQLA AEFRDLGAVS AQTYERLGGH 190 200 210 220 230 240 FDLIINSTSA SLQGELPPLV PALIHADIAI YDMMYGATDT PFIAWAKGQG ARQTVDGLGM 250 260 270 LVEQAAEAFT VWRGIRPGTK QVLRELKRNL GTL

References

[1]

"Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades." Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F. J. Bacteriol. 188:8272-8282(2006) [PubMed: 16980456] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000462 Genomic DNA. Translation: ABK36524.1.
RefSeq	YP_854794.1.
3D structure databases
SMR	A0KEX8. Positions 1-270.
ModBase	Search...
Protein-protein interaction databases
STRING	A0KEX8.
Genome annotation databases
GeneID	4490197.
GenomeReviews	Gene locus AHA_0266 in contig CP000462_GR.
KEGG	aha:AHA_0266.
TIGR	AHA_0266.
Phylogenomic databases
eggNOG	COG0169.
HOGENOM	HBG553408.
OMA	DQYVVFG.
PhylomeDB	A0KEX8.
Family and domain databases
HAMAP	MF_00222. Shikimate_DH_AroE. [Tree]
InterPro	IPR016040. NAD(P)-bd_dom. IPR011342. Quinate/shikimate_5-DH. IPR013708. Shikimate_DH-bd_N. IPR006151. Shikm_DH/Glu-tRNA_Rdtase. [Graphical view]
Pfam	PF01488. Shikimate_DH. 1 hit. PF08501. Shikimate_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00507. aroE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AROE_AERHH

Accession

Primary (citable) accession number: A0KEX8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	December 12, 2006
Last modified:	February 9, 2010
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)