Reviewed, UniProtKB/Swiss-Prot A0KEQ0 (PLSB_AERHH)

Names and origin

Protein names

Recommended name:
    Glycerol-3-phosphate acyltransferase
      Short name=GPAT
    EC=2.3.1.15

Gene names

Name:	plsB
Ordered Locus Names:	AHA_0182

Organism

Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) [Complete proteome] [HAMAP]

Taxonomic identifier

380703 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	807 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. HAMAP MF_00393
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3. HAMAP MF_00393
Subcellular location	Cell membrane; Peripheral membrane protein By similarity HAMAP MF_00393.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity. HAMAP MF_00393
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell membrane Membrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 807	807	Glycerol-3-phosphate acyltransferase HAMAP MF_00393		PRO_1000049427
Regions
	Motif	309 – 314	6	HXXXXD motif HAMAP MF_00393

Sequences

	Sequence		Length	Mass (Da)
	A0KEQ0-1 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 030E2B3C2CCC210E	FASTA	807	91,472
	10 20 30 40 50 60 MSLGQNISRA ILQWPVSGLV NHKSLPENPI TELKLDPARP IVYALKTSSI TDLMTLQQCC 70 80 90 100 110 120 EDLGLPGPFT PLELNGQLLP RYVCLDRPPP LFGKRSKPLP FLQEFHQLLD LHKQDPALDI 130 140 150 160 170 180 QVVPVTLFWG RAPGREGEEA SGWNIISSLA PNRLKKALIV ILKGRENLVR FSPPLSLRHM 190 200 210 220 230 240 ADKHGTDEAI AHKLARVART HFSRQQLAAT GPKLPNRNLL FKQLLDSNVI QQAIEEEAQR 250 260 270 280 290 300 EGISLEKAQK RAHGYMDEIA SDFSYRLIRL GESFLGWLWN KLYRGLSVNG AEKVRQLAQE 310 320 330 340 350 360 GHEIVYVPCH RSHMDYLLLS YVIYHQGMVP PHIAAGINLN FWPAGPIFRH GGAFFIRRTF 370 380 390 400 410 420 KGNPLYSTVF REYLNLLFAK GYSVEFFTEG GRSRTGRLLP PKTGMLAMTL QAMMRGLDRP 430 440 450 460 470 480 VTLVPVYLGY EHVMEVNTYH NELKGSRKEK ESFLQVLGIL RKLRNYGRGF VNFGEPLTLN 490 500 510 520 530 540 NYLNEHVPSW KEHIGEEERP EWMAPTVNQL AELLMTRING AAAVNGLTLS ALALLAAERH 550 560 570 580 590 600 ALTRDELQAQ LNTYLDLLKQ VPYSPHSTIP DEDAKTLLDQ AMELNKFEVS EDKLGQIVSL 610 620 630 640 650 660 DRYQAILLTY YRNNILHLFA MPSLVAALIE RCEGISRSEI VARCIDIYPL LKTELFLRYE 670 680 690 700 710 720 EDELPELVDA LLAELQRQQL IEARDGGFWV NPVNQTRLLL LAESIQETLQ RYAIVLTRVL 730 740 750 760 770 780 AQPRIEAEQL EADGLMMAER LGTLHGINAP EFFDQKLFST LIHTLRSEGY LDPGCKPDLG 790 800 RFQALADNIV PLLSTKIRRT IQAGNRL

References

[1]

"Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades." Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F. J. Bacteriol. 188:8272-8282(2006) [PubMed: 16980456] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000462 Genomic DNA. Translation: ABK36673.1.
RefSeq	YP_854715.1.
3D structure databases
SMR	A0KEQ0. Positions 261-522.
ModBase	Search...
Protein-protein interaction databases
STRING	A0KEQ0.
Genome annotation databases
GeneID	4486812.
GenomeReviews	Gene locus AHA_0182 in contig CP000462_GR.
KEGG	aha:AHA_0182.
TIGR	AHA_0182.
Phylogenomic databases
eggNOG	COG2937.
HOGENOM	HBG296590.
OMA	WNKLYQG.
Family and domain databases
HAMAP	MF_00393. Glyc3P_acyltrans. [Tree]
InterPro	IPR002123. Acyltransferase. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLSB_AERHH

Accession

Primary (citable) accession number: A0KEQ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	December 12, 2006
Last modified:	January 19, 2010
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)