ID   ILVC_AERHH              Reviewed;         493 AA.
AC   A0KEM1;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   12-DEC-2006, sequence version 1.
DT   16-JUN-2009, entry version 22.
DE   RecName: Full=Ketol-acid reductoisomerase;
DE            EC=1.1.1.86;
DE   AltName: Full=Acetohydroxy-acid isomeroreductase;
DE   AltName: Full=Alpha-keto-beta-hydroxylacil reductoisomerase;
GN   Name=ilvC; OrderedLocusNames=AHA_0153;
OS   Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Aeromonadales;
OC   Aeromonadaceae; Aeromonas.
OX   NCBI_TaxID=380703;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16980456; DOI=10.1128/JB.00621-06;
RA   Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J.,
RA   Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M.,
RA   Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F.;
RT   "Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all
RT   trades.";
RL   J. Bacteriol. 188:8272-8282(2006).
CC   -!- CATALYTIC ACTIVITY: (R)-2,3-dihydroxy-3-methylbutanoate + NADP(+)
CC       = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH.
CC   -!- CATALYTIC ACTIVITY: (2R,3R)-2,3-dihydroxy-3-methylpentanoate +
CC       NADP(+) = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH.
CC   -!- PATHWAY: Amino-acid biosynthesis; L-isoleucine biosynthesis; L-
CC       isoleucine from 2-oxobutanoate: step 2/4.
CC   -!- PATHWAY: Amino-acid biosynthesis; L-valine biosynthesis; L-valine
CC       from pyruvate: step 2/4.
CC   -!- SIMILARITY: Belongs to the ketol-acid reductoisomerase family.
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DR   EMBL; CP000462; ABK38758.1; -; Genomic_DNA.
DR   RefSeq; YP_854686.1; -.
DR   GeneID; 4490334; -.
DR   GenomeReviews; CP000462_GR; AHA_0153.
DR   KEGG; aha:AHA_0153; -.
DR   TIGR; AHA_0153; -.
DR   OMA; A0KEM1; LKHGGIT.
DR   GO; GO:0004455; F:ketol-acid reductoisomerase activity; IEA:HAMAP.
DR   GO; GO:0050661; F:NADP or NADPH binding; IEA:InterPro.
DR   GO; GO:0009097; P:isoleucine biosynthetic process; IEA:HAMAP.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0009099; P:valine biosynthetic process; IEA:HAMAP.
DR   HAMAP; MF_00435; -; 1.
DR   InterPro; IPR013023; AcH_isomrdctse.
DR   InterPro; IPR000506; AcH_isomrdctse_C.
DR   InterPro; IPR013116; IlvN.
DR   InterPro; IPR014359; KetolA_reductoisomerase_bac.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   PANTHER; PTHR21371; AcH_isomrdctse; 1.
DR   Pfam; PF01450; IlvC; 1.
DR   Pfam; PF07991; IlvN; 1.
DR   PIRSF; PIRSF000116; IlvC_gammaproteo; 1.
DR   TIGRFAMs; TIGR00465; ilvC; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Branched-chain amino acid biosynthesis;
KW   Complete proteome; NADP; Oxidoreductase.
FT   CHAIN         1    493       Ketol-acid reductoisomerase.
FT                                /FTId=PRO_1000050474.
FT   ACT_SITE    132    132       Potential.
SQ   SEQUENCE   493 AA;  53827 MW;  116A6F2DC97F78C6 CRC64;
     MANYFNTLNL RQQLAQLGKC RFMQREEFAD GCNVLKGKKV VIVGCGAQGL NQGLNMRDSG
     LDISYALRKA AITEKRASWQ KATDNGFAVG TYEELIPTAD LVLNLTPDKQ HSDVVKTVMP
     LMKQGAALGY SHGFNVVEEG QQIRADITVV MVAPKCPGTE VREEYKRGFG VPTLIAVHPE
     NDPKGEGMAI AKAWASATGG DRAGVLESSF VAEVKSDLMG EQTILCGMLQ AGSLLCYDKL
     VAEGTDPAYA GKLIQFGWET ITEALKQGGI SLMMDRLSNP AKLRAFELSE QLKTLMRPLF
     EKHMDDIIAG EFSRGMMADW AEDDAKLFGW REETGKSAFE NAPAFAGKIA EQEYFDNGVV
     MVAMVKAGVE LAFETMVASG IYEESAYYES LHELPLIANT VARKRLYEMN VVISDTAEYG
     NYLFANAAVP LLREHFMPTL KAGDLGASKA EGQSVDNLAL LAANEATRNH PIEKIGQVLR
     GYMKDMKRIA VGG
//