Reviewed, UniProtKB/Swiss-Prot A0KEM1 (ILVC_AERHH)

Names and origin

Protein names

Recommended name:
    Ketol-acid reductoisomerase
    EC=1.1.1.86
Alternative name(s):
    Acetohydroxy-acid isomeroreductase
    Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	AHA_0153

Organism

Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) [Complete proteome] [HAMAP]

Taxonomic identifier

380703 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	493 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 493	493	Ketol-acid reductoisomerase HAMAP MF_00435		PRO_1000050474
Sites
	Active site	132	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	A0KEM1-1 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 116A6F2DC97F78C6	FASTA	493	53,827
	10 20 30 40 50 60 MANYFNTLNL RQQLAQLGKC RFMQREEFAD GCNVLKGKKV VIVGCGAQGL NQGLNMRDSG 70 80 90 100 110 120 LDISYALRKA AITEKRASWQ KATDNGFAVG TYEELIPTAD LVLNLTPDKQ HSDVVKTVMP 130 140 150 160 170 180 LMKQGAALGY SHGFNVVEEG QQIRADITVV MVAPKCPGTE VREEYKRGFG VPTLIAVHPE 190 200 210 220 230 240 NDPKGEGMAI AKAWASATGG DRAGVLESSF VAEVKSDLMG EQTILCGMLQ AGSLLCYDKL 250 260 270 280 290 300 VAEGTDPAYA GKLIQFGWET ITEALKQGGI SLMMDRLSNP AKLRAFELSE QLKTLMRPLF 310 320 330 340 350 360 EKHMDDIIAG EFSRGMMADW AEDDAKLFGW REETGKSAFE NAPAFAGKIA EQEYFDNGVV 370 380 390 400 410 420 MVAMVKAGVE LAFETMVASG IYEESAYYES LHELPLIANT VARKRLYEMN VVISDTAEYG 430 440 450 460 470 480 NYLFANAAVP LLREHFMPTL KAGDLGASKA EGQSVDNLAL LAANEATRNH PIEKIGQVLR 490 GYMKDMKRIA VGG

References

[1]

"Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades." Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F. J. Bacteriol. 188:8272-8282(2006) [PubMed: 16980456] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000462 Genomic DNA. Translation: ABK38758.1.
RefSeq	YP_854686.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4490334.
GenomeReviews	Gene locus AHA_0153 in contig CP000462_GR.
KEGG	aha:AHA_0153.
TIGR	AHA_0153.
Phylogenomic databases
OMA	A0KEM1. LKHGGIT.
Family and domain databases
HAMAP	MF_00435. [Tree]
InterPro	IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013116. IlvN. IPR014359. KetolA_reductoisomerase_bac. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
PIRSF	PIRSF000116. IlvC_gammaproteo. 1 hit.
TIGRFAMs	TIGR00465. ilvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_AERHH

Accession

Primary (citable) accession number: A0KEM1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	December 12, 2006
Last modified:	June 16, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)