A0KEG8 (GPPA_AERHH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase
EC=3.6.1.40

Alternative name(s):
Guanosine pentaphosphate phosphohydrolase
pppGpp-5'-phosphohydrolase

Gene names

Name:	gppA
Ordered Locus Names:	AHA_0096

Organism

Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) [Complete proteome] [HAMAP]

Taxonomic identifier

380703 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas › Aeromonas hydrophila › Aeromonas hydrophila subsp. hydrophila

Protein attributes

Sequence length	496 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Conversion of pppGpp to ppGpp. Guanosine pentaphosphate (pppGpp) is a cytoplasmic signaling molecule which together with ppGpp controls the "stringent response", an adaptive process that allows bacteria to respond to amino acid starvation, resulting in the coordinated regulation of numerous cellular activities By similarity. HAMAP-Rule MF_01550
Catalytic activity	Guanosine 5'-triphosphate,3'-diphosphate + H2O = guanosine 3',5'-bis(diphosphate) + phosphate. HAMAP-Rule MF_01550
Pathway	Purine metabolism; ppGpp biosynthesis; ppGpp from GTP: step 1/2. HAMAP-Rule MF_01550
Sequence similarities	Belongs to the GppA/Ppx family. GppA subfamily.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	guanosine pentaphosphate catabolic process Inferred from electronic annotation. Source: InterPro guanosine tetraphosphate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	guanosine-5'-triphosphate,3'-diphosphate diphosphatase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: InterPro phosphoric diester hydrolase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 496	496	Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase HAMAP-Rule MF_01550		PRO_0000314491

Sequences

	Sequence		Length	Mass (Da)
	A0KEG8 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 3166C56CE0D4D7B0	FASTA	496	55,133
	10 20 30 40 50 60 MHNSPLYAAI DLGSNSFHML VVREVAGALR TVTKVKRKVR LAAGLDPEFR LSRAAMERGW 70 80 90 100 110 120 DCLRLFSEQL QDIPADNIRV VGTATLRLAT NVDEFLSEAE RVLNHSIEII SGEEEAKTIY 130 140 150 160 170 180 EGVSWTSAGE GNRLVIDIGG ASTELVIGEH SEAKLLNSLH MGCVTWLNNH FGDGELSEAR 190 200 210 220 230 240 FKQAIAAAKA VLEKVAEDYR ALGWRTCVGA SGTVQALQEI MLAQGKSERV TLPKLQELMG 250 260 270 280 290 300 QAIACGKLDQ LQLEGLAAER LTVFPSGLAI LIAIFETLGI ESMTLAGGAL REGLIYGLLG 310 320 330 340 350 360 NNHDCDARDR TADSLINRYQ LDKEHAERVR DTAVEAFNQL QPAWRLSKRY GRPILRYAAL 370 380 390 400 410 420 LHEIGLCIEY KKAPQHAAYI IDNIDMPGFT PAQKKLLSAL LFNQRDEFKL EPLEKQGAVT 430 440 450 460 470 480 GRQAIRLARI LRIALILCMR RTQGTVPRFT LEADEDALTL TLPSGWLDEH YLRASELRFE 490 VERQQKMGWP TRLLEA

References

[1]

"Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades." Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H., Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S., Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F. J. Bacteriol. 188:8272-8282(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 7966 / NCIB 9240.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000462 Genomic DNA. Translation: ABK39541.1.
RefSeq	YP_854621.1. NC_008570.1.
3D structure databases
ProteinModelPortal	A0KEG8.
ModBase	Search...
Protein-protein interaction databases
STRING	380703.AHA_0096.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABK39541; ABK39541; AHA_0096.
GeneID	4490991.
KEGG	aha:AHA_0096.
PATRIC	20777654. VBIAerHyd135212_0088.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0248.
HOGENOM	HOG000258672.
KO	K01524.
OMA	ANRRRND.
ProtClustDB	CLSK730186.
Enzyme and pathway databases
BioCyc	AHYD380703:GH2M-247-MONOMER.
UniPathway	UPA00908; UER00884.
Family and domain databases
Gene3D	1.10.3210.10. 1 hit.
HAMAP	MF_01550. GppA.
InterPro	IPR023709. Guo-5TP_3DP_PyrP. IPR006674. HD_domain. IPR003695. Ppx_GppA. [Graphical view]
Pfam	PF02541. Ppx-GppA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPPA_AERHH

Accession

Primary (citable) accession number: A0KEG8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	December 12, 2006
Last modified:	May 1, 2013
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program