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Protein

3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase

Gene

mhpA

Organism
Burkholderia cenocepacia (strain HI2424)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the insertion of one atom of molecular oxygen into position 2 of the phenyl ring of 3-(3-hydroxyphenyl)propionate (3-HPP) and hydroxycinnamic acid (3HCI).UniRule annotation

Catalytic activityi

3-(3-hydroxyphenyl)propanoate + NADH + O2 = 3-(2,3-dihydroxyphenyl)propanoate + H2O + NAD+.UniRule annotation
(2E)-3-(3-hydroxyphenyl)prop-2-enoate + NADH + O2 = (2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + H2O + NAD+.UniRule annotation

Cofactori

FADUniRule annotation

Pathwayi: 3-phenylpropanoate degradation

This protein is involved in the pathway 3-phenylpropanoate degradation, which is part of Aromatic compound metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway 3-phenylpropanoate degradation and in Aromatic compound metabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 39FADUniRule annotationAdd BLAST30
Nucleotide bindingi278 – 288FADUniRule annotationAdd BLAST11

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Keywords - Ligandi

FAD, Flavoprotein, NAD

Enzyme and pathway databases

UniPathwayiUPA00714.

Names & Taxonomyi

Protein namesi
Recommended name:
3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylaseUniRule annotation (EC:1.14.13.127UniRule annotation)
Short name:
3-HCI hydroxylaseUniRule annotation
Short name:
3-HPP hydroxylaseUniRule annotation
Gene namesi
Name:mhpAUniRule annotation
Ordered Locus Names:Bcen2424_6722
OrganismiBurkholderia cenocepacia (strain HI2424)
Taxonomic identifieri331272 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_50001652581 – 5423-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylaseAdd BLAST542

Structurei

3D structure databases

ProteinModelPortaliA0KE38.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PheA/TfdB FAD monooxygenase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000221401.
KOiK05712.
OMAiRRVYTHH.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
HAMAPiMF_01652. MhpA. 1 hit.
InterProiIPR023786. 3-HPP/3HCI_hydroxylase.
IPR002938. FAD-bd.
IPR023753. FAD/NAD-binding_dom.
[Graphical view]
PfamiPF01494. FAD_binding_3. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.

Sequencei

Sequence statusi: Complete.

A0KE38-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKANNRNRTS VAIVGAGPNG AAMANLLGLY GVDTIVVERA PQIVEFPRAV
60 70 80 90 100
GIDDEALRLF QTAGLADELS RDIIQNVPLR MFKANGECFA DIRPSIREFG
110 120 130 140 150
WWRRNIFMQH LAERTLRDAL ARYPHVSLRT GEEVVGLEQD DECVTLQVRA
160 170 180 190 200
ADGQQYELDA DYVVAADGGR SPVREMLGIR LAGTTHPMKW VVVDVKNARL
210 220 230 240 250
DQPCTALNCD PRRPNVCIYL PFNYRRWEFL VFPHEDEEAI AQPESIRALI
260 270 280 290 300
APYVDDVDRI EIVRARTYTH HSRVAERFVA GRVALIGDAA HLSPPWIGQG
310 320 330 340 350
LNAGLRDVGN LAWKLAGVVN GTLHRRVIST YESERRDHAK AMIDLADTFG
360 370 380 390 400
AMLMPTSRLV AFLRDRFLGL ARYAPGLKDY VLQMRFKPMP SYTHGVVVTG
410 420 430 440 450
TSDAVGRMIV QPDVETADGA RRKLDDVLGP WFAIIGWRCD PQACLSDDDR
460 470 480 490 500
AFWTALGAKF VQIVRSRSGT CREQRIASAH DSVCVEDVDN AMAEWFDRHA
510 520 530 540
APLVVVRPDR YVAAQTDAAG MAGVTAAFQA FAARQRETAD VC
Length:542
Mass (Da):60,297
Last modified:December 12, 2006 - v1
Checksum:i6A67D4F054BF5F9B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000460 Genomic DNA. Translation: ABK13451.1.
RefSeqiWP_011549881.1. NC_008544.1.

Genome annotation databases

EnsemblBacteriaiABK13451; ABK13451; Bcen2424_6722.
KEGGibch:Bcen2424_6722.
PATRICi19071186. VBIBurCen15205_6903.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000460 Genomic DNA. Translation: ABK13451.1.
RefSeqiWP_011549881.1. NC_008544.1.

3D structure databases

ProteinModelPortaliA0KE38.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK13451; ABK13451; Bcen2424_6722.
KEGGibch:Bcen2424_6722.
PATRICi19071186. VBIBurCen15205_6903.

Phylogenomic databases

HOGENOMiHOG000221401.
KOiK05712.
OMAiRRVYTHH.

Enzyme and pathway databases

UniPathwayiUPA00714.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
HAMAPiMF_01652. MhpA. 1 hit.
InterProiIPR023786. 3-HPP/3HCI_hydroxylase.
IPR002938. FAD-bd.
IPR023753. FAD/NAD-binding_dom.
[Graphical view]
PfamiPF01494. FAD_binding_3. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiMHPA_BURCH
AccessioniPrimary (citable) accession number: A0KE38
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: December 12, 2006
Last modified: November 2, 2016
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.