Reviewed, UniProtKB/Swiss-Prot A0KDG4 (GLGB_BURCH)

Names and origin

Protein names

Recommended name:
    1,4-alpha-glucan-branching enzyme
    EC=2.4.1.18
Alternative name(s):
    Glycogen-branching enzyme
      Short name=BE
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase

Gene names

Name:	glgB
Ordered Locus Names:	Bcen2424_6497

Organism

Burkholderia cenocepacia (strain HI2424) [Complete proteome] [HAMAP]

Taxonomic identifier

331272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	736 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity.
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Glycogen biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 736	736	1,4-alpha-glucan-branching enzyme HAMAP MF_00685		PRO_1000044969
Sites
	Active site	310	1	By similarity
	Active site	345	1	By similarity
	Active site	350	1	By similarity
	Active site	413	1	By similarity
	Active site	415	1	By similarity
	Active site	470	1	By similarity
	Active site	537	1	By similarity
	Active site	538	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0KDG4-1 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: F77DAC25AED9110B	FASTA	736	81,251
	10 20 30 40 50 60 MTDTLFDRAD IDALLAGRHP DPFACLGPHR HDDQIVVRAL LPGAERVRAL SPDGAELGTL 70 80 90 100 110 120 ACVDRAGCFA GTIAHDGGAP HYLLSIDWPD AHQVTDDAYA FGTLLDEAAL ARFSAGDPEA 130 140 150 160 170 180 VLDCLGATPV RIDDTDGVRF AVWAPSAQRV SVVGDFNAWD GRRHPMRLRR PSGVWELFVP 190 200 210 220 230 240 GIGAGERYKY ELCAADGRVL PHKADPCARA TEAPPRTASV VADVAALHAF AWHDDGWMHA 250 260 270 280 290 300 RPRHDDRYRV PWSIYEVHPE SWQRIPEQMD RSATWDELAE RLIPYVKGMG FTHVEFMPIA 310 320 330 340 350 360 EYPFGGSWGY QPLAQFAPSA RFGPVEGFAR FVDRAHAAGI GVLVDWVPAH FPNDAHGLAQ 370 380 390 400 410 420 FDGSALYEHA DPREGMHPDW NTCVFNVGRT EVSAFLVASA LAWARRYHVD GIRVDAVASM 430 440 450 460 470 480 LYRDYSRKEG EWVPNVYGGR ENLESVAFLR RLNDTLHGDA APAGVVTVAE ESTAWPGVTA 490 500 510 520 530 540 PTADGGLGFD FKWNMGWMHD TLAYLHEDPI HRRYHHDRMT FGLVYAFSER FVLPLSHDEV 550 560 570 580 590 600 VHGKGSLVAK MPGDAWQRLA TLRAYFGFMW AHPGKKLLFM GSEFAQWSEF AHDATPHWDL 610 620 630 640 650 660 LDAPAHRGVQ RLVRDLNRAY AAEPALHALD CHAAGFAWLI GDDRDNSVFA FARRDDTGRL 670 680 690 700 710 720 VVAVCNFTPV PRAGYRLGLP APGHWRELMN TDAASYGGTN AGNDGAVWAE AVPAHGEAWS 730 ASLRLPPLAT LWLTPA

References

[1]

"Complete sequence of chromosome 3 of Burkholderia cenocepacia HI2424." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., LiPuma J.J., Gonzalez C.F., Konstantinidis K., Tiedje J.M., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000460 Genomic DNA. Translation: ABK13227.1.
RefSeq	YP_840120.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A0KDG4.
Genome annotation databases
GeneID	4454650.
GenomeReviews	Gene locus Bcen2424_6497 in contig CP000460_GR.
KEGG	bch:Bcen2424_6497.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	A0KDG4.
OMA	PKTASIV.
Family and domain databases
HAMAP	MF_00685. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz_core. IPR006048. A-amylase_b_C. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat. IPR004193. Glyco_hydro_13_N. IPR013783. Ig-like_fold. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
TIGRFAMs	TIGR01515. branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB_BURCH

Accession

Primary (citable) accession number: A0KDG4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	December 12, 2006
Last modified:	November 3, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)