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A0KDG4

- GLGB_BURCH

UniProt

A0KDG4 - GLGB_BURCH

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Protein
1,4-alpha-glucan branching enzyme GlgB
Gene
glgB, Bcen2424_6497
Organism
Burkholderia cenocepacia (strain HI2424)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei415 – 4151Nucleophile By similarity
Active sitei470 – 4701Proton donor By similarity

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. glycogen biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

BioCyciBCEN331272:GHR7-6582-MONOMER.
UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgB (EC:2.4.1.18)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name:
BE
Gene namesi
Name:glgB
Ordered Locus Names:Bcen2424_6497
OrganismiBurkholderia cenocepacia (strain HI2424)
Taxonomic identifieri331272 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
ProteomesiUP000000776: Chromosome 3

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7367361,4-alpha-glucan branching enzyme GlgBUniRule annotation
PRO_1000044969Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi331272.Bcen2424_6497.

Structurei

3D structure databases

ProteinModelPortaliA0KDG4.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiHERCPHA.
OrthoDBiEOG6JX7GT.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

A0KDG4-1 [UniParc]FASTAAdd to Basket

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MTDTLFDRAD IDALLAGRHP DPFACLGPHR HDDQIVVRAL LPGAERVRAL    50
SPDGAELGTL ACVDRAGCFA GTIAHDGGAP HYLLSIDWPD AHQVTDDAYA 100
FGTLLDEAAL ARFSAGDPEA VLDCLGATPV RIDDTDGVRF AVWAPSAQRV 150
SVVGDFNAWD GRRHPMRLRR PSGVWELFVP GIGAGERYKY ELCAADGRVL 200
PHKADPCARA TEAPPRTASV VADVAALHAF AWHDDGWMHA RPRHDDRYRV 250
PWSIYEVHPE SWQRIPEQMD RSATWDELAE RLIPYVKGMG FTHVEFMPIA 300
EYPFGGSWGY QPLAQFAPSA RFGPVEGFAR FVDRAHAAGI GVLVDWVPAH 350
FPNDAHGLAQ FDGSALYEHA DPREGMHPDW NTCVFNVGRT EVSAFLVASA 400
LAWARRYHVD GIRVDAVASM LYRDYSRKEG EWVPNVYGGR ENLESVAFLR 450
RLNDTLHGDA APAGVVTVAE ESTAWPGVTA PTADGGLGFD FKWNMGWMHD 500
TLAYLHEDPI HRRYHHDRMT FGLVYAFSER FVLPLSHDEV VHGKGSLVAK 550
MPGDAWQRLA TLRAYFGFMW AHPGKKLLFM GSEFAQWSEF AHDATPHWDL 600
LDAPAHRGVQ RLVRDLNRAY AAEPALHALD CHAAGFAWLI GDDRDNSVFA 650
FARRDDTGRL VVAVCNFTPV PRAGYRLGLP APGHWRELMN TDAASYGGTN 700
AGNDGAVWAE AVPAHGEAWS ASLRLPPLAT LWLTPA 736
Length:736
Mass (Da):81,251
Last modified:December 12, 2006 - v1
Checksum:iF77DAC25AED9110B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000460 Genomic DNA. Translation: ABK13227.1.
RefSeqiWP_011695010.1. NC_008544.1.
YP_840120.1. NC_008544.1.

Genome annotation databases

EnsemblBacteriaiABK13227; ABK13227; Bcen2424_6497.
GeneIDi4454650.
KEGGibch:Bcen2424_6497.
PATRICi19070721. VBIBurCen15205_6671.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000460 Genomic DNA. Translation: ABK13227.1 .
RefSeqi WP_011695010.1. NC_008544.1.
YP_840120.1. NC_008544.1.

3D structure databases

ProteinModelPortali A0KDG4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 331272.Bcen2424_6497.

Protein family/group databases

CAZyi CBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABK13227 ; ABK13227 ; Bcen2424_6497 .
GeneIDi 4454650.
KEGGi bch:Bcen2424_6497.
PATRICi 19070721. VBIBurCen15205_6671.

Phylogenomic databases

eggNOGi COG0296.
HOGENOMi HOG000283037.
KOi K00700.
OMAi HERCPHA.
OrthoDBi EOG6JX7GT.

Enzyme and pathway databases

UniPathwayi UPA00164 .
BioCyci BCEN331272:GHR7-6582-MONOMER.

Family and domain databases

Gene3Di 2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_00685. GlgB.
InterProi IPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view ]
PANTHERi PTHR10357. PTHR10357. 1 hit.
Pfami PF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view ]
PIRSFi PIRSF000463. GlgB. 1 hit.
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsi TIGR01515. branching_enzym. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of chromosome 3 of Burkholderia cenocepacia HI2424."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.
    , Kim E., LiPuma J.J., Gonzalez C.F., Konstantinidis K., Tiedje J.M., Richardson P.
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HI2424.

Entry informationi

Entry nameiGLGB_BURCH
AccessioniPrimary (citable) accession number: A0KDG4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: December 12, 2006
Last modified: September 3, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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