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A0KCL8 (A0KCL8_BURCH) Unreviewed, UniProtKB/TrEMBL

Last modified December 14, 2011. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Adenylosuccinate synthetase 2 HAMAP MF_00011
Short name=AMPSase 2 HAMAP MF_00011
Short name=AdSS 2 HAMAP MF_00011
EC=6.3.4.4 HAMAP MF_00011
Alternative name(s):
IMP--aspartate ligase 2 HAMAP MF_00011
Gene names
Name:purA2 HAMAP MF_00011
Ordered Locus Names:Bcen2424_6199
OrganismBurkholderia cenocepacia (strain HI2424) [Complete proteome] [HAMAP]
Taxonomic identifier331272 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length453 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Plays an important role in the de novo pathway of purine nucleotide biosynthesis By similarity. RuleBase RU000520

Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP By similarity. HAMAP MF_00011

Catalytic activity

GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP. HAMAP MF_00011 RuleBase RU000520

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00011

Pathway

Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. HAMAP MF_00011 RuleBase RU000520

Subunit structure

Homodimer By similarity. HAMAP MF_00011

Subcellular location

Cytoplasm By similarity HAMAP MF_00011.

Sequence similarities

Belongs to the adenylosuccinate synthetase family. RuleBase RU004163 HAMAP MF_00011

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding33 – 397GTP By similarity HAMAP MF_00011
Nucleotide binding61 – 633GTP By similarity HAMAP MF_00011
Nucleotide binding350 – 3523GTP By similarity HAMAP MF_00011
Nucleotide binding432 – 4343GTP By similarity HAMAP MF_00011
Region34 – 374IMP binding By similarity HAMAP MF_00011
Region59 – 624IMP binding By similarity HAMAP MF_00011
Region318 – 3247Substrate binding By similarity HAMAP MF_00011

Sites

Active site341Proton acceptor By similarity HAMAP MF_00011
Active site621Proton donor By similarity HAMAP MF_00011
Metal binding341Magnesium By similarity HAMAP MF_00011
Metal binding611Magnesium; via carbonyl oxygen By similarity HAMAP MF_00011
Binding site1491IMP By similarity HAMAP MF_00011
Binding site1631IMP; shared with dimeric partner By similarity HAMAP MF_00011
Binding site2431IMP By similarity HAMAP MF_00011
Binding site2581IMP By similarity HAMAP MF_00011
Binding site3221IMP By similarity HAMAP MF_00011
Binding site3241GTP By similarity HAMAP MF_00011

Sequences

Sequence LengthMass (Da)Tools
A0KCL8 [UniParc].

Last modified December 12, 2006. Version 1.
Checksum: 26D0E1316B4FB015

FASTA45348,250
        10         20         30         40         50         60 
MAHKRPTWPN QCLNFSHGVR SMPNVVVVGA QWGDEGKGRV VDWLAAQADL VARYNGGHNA 

        70         80         90        100        110        120 
GHTLVVGGNT YKLALLPSGI VRGKRGVIGN GVALDPEALL AEIGRMAELG LPVTPDNLSI 

       130        140        150        160        170        180 
AENATLVLPI HRAIDEAQER LRREPIGTTL RGIGPAYEDK VGRRGLRVGD LAEPDGLAAK 

       190        200        210        220        230        240 
LDVLVDHHNA WFRGLGLDEY DRDAMLATLV ALAPKILPFV RPVWADLNDA TDRGERILFE 

       250        260        270        280        290        300 
GSQAVMLDID WGTYPFVTSS GTVASAAAAG TGLGAAKLGH VLGVTKAYAT RVGGGPFLTE 

       310        320        330        340        350        360 
LTDATGETLR ARGQEFGVNT GRPRRCGWLD AAQLRQAVRI SGIDSLALTK LDVLDGFESI 

       370        380        390        400        410        420 
ALCVGYEFDG ARVDHLPASL DAQSRAKPLY ERFEGWQGSV KGVRERAALP RAAQDFIARI 

       430        440        450 
EAVAGAPVSM ITTGAERDDT IVLRDPFDAA AAA

« Hide

References

[1]"Complete sequence of chromosome 3 of Burkholderia cenocepacia HI2424."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Kim E., LiPuma J.J., Gonzalez C.F., Konstantinidis K., Tiedje J.M., Richardson P.
Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000460 Genomic DNA. Translation: ABK12931.1.
RefSeqYP_839824.1. NC_008544.1.

3D structure databases

ProteinModelPortalA0KCL8.
ModBaseSearch...

Protein-protein interaction databases

STRINGA0KCL8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4454154.
GenomeReviewsGene locus Bcen2424_6199 in contig CP000460_GR.
KEGGbch:Bcen2424_6199.
PATRIC19070058. VBIBurCen15205_6345.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0104.
HOGENOMHBG658237.
OMAPIHRAID.
ProtClustDBCLSK941367.

Family and domain databases

HAMAPMF_00011. Adenylosucc_synth.
[Tree]
InterProIPR018220. Adenylosuccinate_synthase_AS.
IPR001114. Adenylosuccinate_synthetase.
[Graphical view]
KOK01939.
PANTHERPTHR11846. Asucc_synthtase. 1 hit.
PfamPF00709. Adenylsucc_synt. 1 hit.
[Graphical view]
SMARTSM00788. Adenylsucc_synt. 1 hit.
[Graphical view]
TIGRFAMsTIGR00184. PurA. 1 hit.
PROSITEPS01266. ADENYLOSUCCIN_SYN_1. 1 hit.
PS00513. ADENYLOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameA0KCL8_BURCH
AccessionPrimary (citable) accession number: A0KCL8
Entry history
Integrated into UniProtKB/TrEMBL: December 12, 2006
Last sequence update: December 12, 2006
Last modified: December 14, 2011
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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