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A0KB05

- BIOB_BURCH

UniProt

A0KB05 - BIOB_BURCH

Protein

Biotin synthase

Gene

bioB

Organism
Burkholderia cenocepacia (strain HI2424)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 53 (01 Oct 2014)
      Sequence version 1 (12 Dec 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi70 – 701Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi74 – 741Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi77 – 771Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi114 – 1141Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi145 – 1451Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi205 – 2051Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi277 – 2771Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciBCEN331272:GHR7-3005-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:Bcen2424_2934
    OrganismiBurkholderia cenocepacia (strain HI2424)
    Taxonomic identifieri331272 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
    ProteomesiUP000000776: Chromosome 1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 339339Biotin synthasePRO_0000381261Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi331272.Bcen2424_2934.

    Structurei

    3D structure databases

    ProteinModelPortaliA0KB05.
    SMRiA0KB05. Positions 23-331.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239957.
    KOiK01012.
    OMAiDETQALC.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A0KB05-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTQAQTAAVQ PAAIPVAAPA SQRWRVADVV ALFELPFNDL MFRAQQVHRE    50
    HFDANAVQLS TLLSIKTGGC EEDCGYCSQS SHHDTGLKAE KLMDVDTVLD 100
    AARAAKANGA SRFCMGAAWR NPKERHMPAL TEMVRGVKEL GLETCMTLGM 150
    LEDEQAQQLA DAGLDYYNHN LDTSPEFYGQ VISTRTYQDR LDTLDRVRDA 200
    GINVCCGGII GMGESRRERA GLISQLANLN PYPESVPINN LVAIEGTPLE 250
    GTAPLDPFEF VRTIAVARIT MPKAVVRLSA GREQLDDAMQ AMCFLAGANS 300
    MFYGDQLLTT SNPQTQRDRA LFERLGIRAS QADALSDNA 339
    Length:339
    Mass (Da):37,014
    Last modified:December 12, 2006 - v1
    Checksum:i1CB08D807073166F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000458 Genomic DNA. Translation: ABK09682.1.
    RefSeqiYP_836575.1. NC_008542.1.

    Genome annotation databases

    EnsemblBacteriaiABK09682; ABK09682; Bcen2424_2934.
    GeneIDi4448466.
    KEGGibch:Bcen2424_2934.
    PATRICi19063349. VBIBurCen15205_3009.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000458 Genomic DNA. Translation: ABK09682.1 .
    RefSeqi YP_836575.1. NC_008542.1.

    3D structure databases

    ProteinModelPortali A0KB05.
    SMRi A0KB05. Positions 23-331.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 331272.Bcen2424_2934.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABK09682 ; ABK09682 ; Bcen2424_2934 .
    GeneIDi 4448466.
    KEGGi bch:Bcen2424_2934.
    PATRICi 19063349. VBIBurCen15205_3009.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239957.
    KOi K01012.
    OMAi DETQALC.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci BCEN331272:GHR7-3005-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of chromosome 1 of Burkholderia cenocepacia HI2424."
      Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.
      , Kim E., LiPuma J.J., Gonzalez C.F., Konstantinidis K., Tiedje J.M., Richardson P.
      Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: HI2424.

    Entry informationi

    Entry nameiBIOB_BURCH
    AccessioniPrimary (citable) accession number: A0KB05
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: December 12, 2006
    Last modified: October 1, 2014
    This is version 53 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3