Reviewed, UniProtKB/Swiss-Prot A0KAZ7 (ACEK_BURCH)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase kinase/phosphatase
      Short name=IDH kinase/phosphatase
      Short name=IDHK/P
    EC=2.7.11.5
    EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	Bcen2424_2926

Organism

Burkholderia cenocepacia (strain HI2424) [Complete proteome] [HAMAP]

Taxonomic identifier

331272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	605 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity.
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the aceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding NADP Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: HAMAP phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 605	605	Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747		PRO_0000288283
Regions
	Nucleotide binding	327 – 333	7	ATP By similarity
Sites
	Active site	383	1	By similarity
	Binding site	348	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0KAZ7-1 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 046D10BA2A519ECA	FASTA	605	69,950
	10 20 30 40 50 60 MNHFPKLLSS QIGFDVAQTM LEYFDRHYRI FREAAVDAKT LFERGDWHGL QRLARERITS 70 80 90 100 110 120 YDERVKECVE VLEDEYDAEN IDDEVWQQIK LHYIGLLTSH RQPECAETFF NSVCCKILHR 130 140 150 160 170 180 SYFSNDFIFV RPAISTEYLE NDEPAAKPTY RAYYPGTDGL AVTLERIVTN FQLDPPFEDL 190 200 210 220 230 240 TRDIGCVMQA IDDEFGHFDE APNFQIHVLS SLFFRNKSAY IVGRIINADR VLPFAVPIRH 250 260 270 280 290 300 VRPGVLALDT VLLRRDLLQI IFSFSHSYFL VDMGVPSAYV DFLCTIMPGK PKAEIYTSVG 310 320 330 340 350 360 LQKQGKNLFY RDLLHHLSHS SDRFIIAPGI KGLVMLVFTL PSFPYVFKII KDHFPPPKET 370 380 390 400 410 420 TRAQIMEKYQ LVKRHDRLGR MADTLEYSSV ALPLARLDHA LVRELEKEVP SLLEYEDDKL 430 440 450 460 470 480 VIKHLYIERR MTPLNLYLQN GSDADVEHGV KEYGNAVKEL MKANIFPGDM LYKNFGVTRH 490 500 510 520 530 540 GRVVFYDYDE IEYLTDCNVR RVPPPRNEED ELSGEPWYTV GPHDIFPETY GPFLLGDPRV 550 560 570 580 590 600 RSVFMKHHAD FFDPALWQAS KDKLMQGELP DFYPYDATLR FSVRYPARFG ATGENDGAGD AQRAA

References

[1]

"Complete sequence of chromosome 1 of Burkholderia cenocepacia HI2424." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., LiPuma J.J., Gonzalez C.F., Konstantinidis K., Tiedje J.M., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000458 Genomic DNA. Translation: ABK09674.1.
RefSeq	YP_836567.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A0KAZ7.
Genome annotation databases
GeneID	4450935.
GenomeReviews	Gene locus Bcen2424_2926 in contig CP000458_GR.
KEGG	bch:Bcen2424_2926.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	A0KAZ7.
OMA	FYRDFLH.
Family and domain databases
HAMAP	MF_00747. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACEK_BURCH

Accession

Primary (citable) accession number: A0KAZ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	December 12, 2006
Last modified:	November 3, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)