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A0KAG0

- QUEF_BURCH

UniProt

A0KAG0 - QUEF_BURCH

Protein

NADPH-dependent 7-cyano-7-deazaguanine reductase

Gene

queF

Organism
Burkholderia cenocepacia (strain HI2424)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 60 (01 Oct 2014)
      Sequence version 1 (12 Dec 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1).UniRule annotation

    Catalytic activityi

    7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei183 – 1831Thioimide intermediateUniRule annotation
    Active sitei190 – 1901Proton donorUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi82 – 832NADPHUniRule annotation
    Nucleotide bindingi251 – 2522NADPHUniRule annotation

    GO - Molecular functioni

    1. oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Source: InterPro
    2. preQ1 synthase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. queuosine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Queuosine biosynthesis

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BioCyciBCEN331272:GHR7-2808-MONOMER.
    UniPathwayiUPA00392.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADPH-dependent 7-cyano-7-deazaguanine reductaseUniRule annotation (EC:1.7.1.13UniRule annotation)
    Alternative name(s):
    7-cyano-7-carbaguanine reductaseUniRule annotation
    NADPH-dependent nitrile oxidoreductaseUniRule annotation
    PreQ(0) reductaseUniRule annotation
    Gene namesi
    Name:queFUniRule annotation
    Ordered Locus Names:Bcen2424_2737
    OrganismiBurkholderia cenocepacia (strain HI2424)
    Taxonomic identifieri331272 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
    ProteomesiUP000000776: Chromosome 1

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 276276NADPH-dependent 7-cyano-7-deazaguanine reductasePRO_1000062328Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi331272.Bcen2424_2737.

    Structurei

    3D structure databases

    ProteinModelPortaliA0KAG0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni80 – 823Substrate bindingUniRule annotation
    Regioni222 – 2232Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0780.
    HOGENOMiHOG000273755.
    KOiK06879.
    OMAiSGFHEQC.
    OrthoDBiEOG6D5G0M.

    Family and domain databases

    HAMAPiMF_00817. QueF_type2.
    InterProiIPR029500. QueF.
    IPR029139. QueF_N.
    IPR016428. QueF_type2.
    [Graphical view]
    PfamiPF14489. QueF. 1 hit.
    PF14819. QueF_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
    TIGRFAMsiTIGR03138. QueF. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A0KAG0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNPEHSPLGK ATVYAAQYDA SLLFPIPRAG AREQLGITSA LPFFGTDIWN    50
    AYELSWLNAR GKPQVAIATF YVPAESPNIV ESKSFKLYLG SFAQSKFDSV 100
    DAVRDVLKRD VSAACGASVS VQLVSPHDFA KLEMDELDGL SLDRLDLDTD 150
    VYEPDPSLLS AADGENEAPV EETLVSDLLR SNCPVTGQPD WGSVQIHYVG 200
    PQIDHAGLLR YIISFRNHTG FHEQCVERIF LDILHACKPV KLAVYARYTR 250
    RGGLDINPFR TNYNQPMPDN ARTARQ 276
    Length:276
    Mass (Da):30,572
    Last modified:December 12, 2006 - v1
    Checksum:i29A97CE9C6A4D387
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000458 Genomic DNA. Translation: ABK09487.1.
    RefSeqiWP_011546193.1. NC_008542.1.
    YP_836380.1. NC_008542.1.

    Genome annotation databases

    EnsemblBacteriaiABK09487; ABK09487; Bcen2424_2737.
    GeneIDi4451141.
    KEGGibch:Bcen2424_2737.
    PATRICi19062921. VBIBurCen15205_2796.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000458 Genomic DNA. Translation: ABK09487.1 .
    RefSeqi WP_011546193.1. NC_008542.1.
    YP_836380.1. NC_008542.1.

    3D structure databases

    ProteinModelPortali A0KAG0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 331272.Bcen2424_2737.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABK09487 ; ABK09487 ; Bcen2424_2737 .
    GeneIDi 4451141.
    KEGGi bch:Bcen2424_2737.
    PATRICi 19062921. VBIBurCen15205_2796.

    Phylogenomic databases

    eggNOGi COG0780.
    HOGENOMi HOG000273755.
    KOi K06879.
    OMAi SGFHEQC.
    OrthoDBi EOG6D5G0M.

    Enzyme and pathway databases

    UniPathwayi UPA00392 .
    BioCyci BCEN331272:GHR7-2808-MONOMER.

    Family and domain databases

    HAMAPi MF_00817. QueF_type2.
    InterProi IPR029500. QueF.
    IPR029139. QueF_N.
    IPR016428. QueF_type2.
    [Graphical view ]
    Pfami PF14489. QueF. 1 hit.
    PF14819. QueF_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
    TIGRFAMsi TIGR03138. QueF. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of chromosome 1 of Burkholderia cenocepacia HI2424."
      Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.
      , Kim E., LiPuma J.J., Gonzalez C.F., Konstantinidis K., Tiedje J.M., Richardson P.
      Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: HI2424.

    Entry informationi

    Entry nameiQUEF_BURCH
    AccessioniPrimary (citable) accession number: A0KAG0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: December 12, 2006
    Last modified: October 1, 2014
    This is version 60 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3