A0KA02 (T23O_BURCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan 2,3-dioxygenase
Short name=TDO
EC=1.13.11.11

Alternative name(s):
Tryptamin 2,3-dioxygenase
Tryptophan oxygenase
Short name=TO
Short name=TRPO
Tryptophan pyrrolase
Tryptophanase

Gene names

Name:	kynA
Ordered Locus Names:	Bcen2424_2579

Organism

Burkholderia cenocepacia (strain HI2424) [Complete proteome] [HAMAP]

Taxonomic identifier

331272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring By similarity.
Catalytic activity	L-tryptophan + O2 = N-formyl-L-kynurenine.
Cofactor	Binds 2 heme groups per tetramer By similarity.
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the tryptophan 2,3-dioxygenase family.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Ligand	Heme Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein homotetramerization Inferred from sequence or structural similarity. Source: UniProtKB tryptophan catabolic process to kynurenine Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	heme binding Inferred from sequence or structural similarity. Source: UniProtKB tryptophan 2,3-dioxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 311	311	Tryptophan 2,3-dioxygenase		PRO_0000360099
Regions
	Region	55 – 59	5	Substrate binding By similarity
	Region	80 – 84	5	Substrate binding By similarity
Sites
	Metal binding	269	1	Iron (heme axial ligand) By similarity
	Binding site	142	1	Substrate By similarity
	Binding site	146	1	Substrate By similarity
	Binding site	153	1	Heme By similarity
	Binding site	283	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0KA02 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: B4FD433152605353	FASTA	311	35,455
	10 20 30 40 50 60 MQPPGGDAPA GCPFSGARAA QPAQAPHEAP HVPGEADAQA GWHNAQLDFS KSMSYGDYLS 70 80 90 100 110 120 LNSILDAQHP LSPDHNEMLF IIQHQTSELW MKLALFELRG ALDAVRTDAL PPAFKMLARV 130 140 150 160 170 180 SRILEQLVQA WNVLSTMTPS EYSAMRPYLG QSSGFQSYQY RQLEFLLGNK NAQMLQPHAH 190 200 210 220 230 240 RPDILEQVRA TLEAPSFYDE VVRLLARRGF PIAAERLERD WTQPTRHDET VEAAWLEVYR 250 260 270 280 290 300 HPQQHWELYE MAEELVDLED AFRQWRFRHV TTVERIIGFK QGTGGTSGAP YLRKMLDVVL 310 FPELWHVRTT L

References

[1]

"Complete sequence of chromosome 1 of Burkholderia cenocepacia HI2424." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., LiPuma J.J., Gonzalez C.F., Konstantinidis K., Tiedje J.M., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HI2424.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000458 Genomic DNA. Translation: ABK09329.1.
RefSeq	YP_836222.1. NC_008542.1.
3D structure databases
ProteinModelPortal	A0KA02.
SMR	A0KA02. Positions 42-311.
ModBase	Search...
Protein-protein interaction databases
STRING	A0KA02.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4449920.
GenomeReviews	Gene locus Bcen2424_2579 in contig CP000458_GR.
KEGG	bch:Bcen2424_2579.
PATRIC	19062588. VBIBurCen15205_2631.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3483.
HOGENOM	HBG647485.
OMA	QYREIEF.
ProtClustDB	CLSK930429.
Enzyme and pathway databases
BioCyc	BCEN331272:BCEN2424_2579-MONOMER.
Family and domain databases
InterPro	IPR017485. Trp_2-3-dOase_bac. IPR004981. Trp_2_3_dOase. [Graphical view]
KO	K00453.
PANTHER	PTHR10138. Trp_2_3_dOase. 1 hit.
Pfam	PF03301. Trp_dioxygenase. 1 hit. [Graphical view]
TIGRFAMs	TIGR03036. Trp_2_3_diox. 1 hit.
ProtoNet	Search...

Entry information

Entry name

T23O_BURCH

Accession

Primary (citable) accession number: A0KA02

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	December 12, 2006
Last modified:	December 14, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program