A0K9X7 (MURB_BURCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylenolpyruvoylglucosamine reductase
EC=1.1.1.158

Alternative name(s):
UDP-N-acetylmuramate dehydrogenase

Gene names

Name:	murB
Ordered Locus Names:	Bcen2424_2554

Organism

Burkholderia cenocepacia (strain HI2424) [Complete proteome] [HAMAP]

Taxonomic identifier

331272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00037
Catalytic activity	UDP-N-acetylmuramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH. HAMAP MF_00037
Cofactor	FAD By similarity. HAMAP MF_00037
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00037
Subcellular location	Cytoplasm By similarity HAMAP MF_00037.
Sequence similarities	Belongs to the MurB family. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: EC flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 349	349	UDP-N-acetylenolpyruvoylglucosamine reductase HAMAP MF_00037		PRO_1000002869
Regions
	Domain	25 – 197	173	FAD-binding PCMH-type
Sites
	Active site	173	1	By similarity
	Active site	249	1	Proton donor By similarity
	Active site	345	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0K9X7 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 718F00A88A5853FD	FASTA	349	37,729
	10 20 30 40 50 60 MPMPPDDSAL SLLPDHPLAA HNTFGIAARA RFAARITQAA QFEALHRDPR VANLPQLVLG 70 80 90 100 110 120 GGSNIVFTRD FDGVVLLDEI AGRRVVREDD DAWYVEAGGG ENWHAFVAWT LEHGMPGLEN 130 140 150 160 170 180 LALIPGTVGA APIQNIGAYG LEMKAYFDSL VAVELATGRR ERFDAARCAF GYRDSFFKRE 190 200 210 220 230 240 GRGRFAIVAV TFRLPKQWTP RLGYADVTRE LDARGITPEA ATPRDVFDAV VAIRRAKLPD 250 260 270 280 290 300 PLVLGNAGSF FKNPVIDAAQ FDALRARAPD VVSYPQPDGQ VKLAAGWLID RCGWKGRALG 310 320 330 340 AAAVHDRQAL VLVNRGGATG ADVLALARAI QADVRAQFGV ELEAEPVCL

References

[1]

"Complete sequence of chromosome 1 of Burkholderia cenocepacia HI2424." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., LiPuma J.J., Gonzalez C.F., Konstantinidis K., Tiedje J.M., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HI2424.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000458 Genomic DNA. Translation: ABK09304.1.
RefSeq	YP_836197.1. NC_008542.1.
3D structure databases
ProteinModelPortal	A0K9X7.
ModBase	Search...
Protein-protein interaction databases
STRING	A0K9X7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4449930.
GenomeReviews	Gene locus Bcen2424_2554 in contig CP000458_GR.
KEGG	bch:Bcen2424_2554.
PATRIC	19062538. VBIBurCen15205_2606.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0812.
HOGENOM	HBG686573.
OMA	IEQAGWK.
ProtClustDB	PRK00046.
Enzyme and pathway databases
BioCyc	BCEN331272:BCEN2424_2554-MONOMER.
Family and domain databases
HAMAP	MF_00037. MurB. [Tree]
InterPro	IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR016167. FAD-bd_2_sub1. IPR003170. MurB. IPR011601. MurB_C. IPR006094. Oxid_FAD_bind_N. [Graphical view]
Gene3D	G3DSA:3.30.465.10. CO_DH_flavoprot_FAD-bd_sub2. 1 hit. G3DSA:3.30.43.10. FAD-binding_2_sub1. 1 hit. G3DSA:3.90.78.10. MurB_C. 1 hit.
KO	K00075.
PANTHER	PTHR21071. MurB. 1 hit.
Pfam	PF01565. FAD_binding_4. 1 hit. PF02873. MurB_C. 1 hit. [Graphical view]
SUPFAM	SSF56176. FAD-binding_2. 1 hit. SSF56194. MurB_C. 1 hit.
TIGRFAMs	TIGR00179. MurB. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MURB_BURCH

Accession

Primary (citable) accession number: A0K9X7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	December 12, 2006
Last modified:	December 14, 2011
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program