Skip Header

Contribute Send feedback
Read comments (?) or add your own

A0K9L0 (SYM_BURCH) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Methionine--tRNA ligase
EC=6.1.1.10
Alternative name(s):
Methionyl-tRNA synthetase
Short name=MetRS
Gene names
Name:metG
Ordered Locus Names:Bcen2424_2437
OrganismBurkholderia cenocepacia (strain HI2424) [Complete proteome] [HAMAP]
Taxonomic identifier331272 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length718 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP MF_00098

Catalytic activity

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_00098

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP MF_00098

Subunit structure

Homodimer By similarity. HAMAP MF_00098

Subcellular location

Cytoplasm By similarity HAMAP MF_00098.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.

Contains 1 tRNA-binding domain.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
RNA-binding
Zinc
tRNA-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processmethionyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

methionine-tRNA ligase activity

Inferred from electronic annotation. Source: EC

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 718718Methionine--tRNA ligase HAMAP MF_00098
PRO_0000331788

Regions

Domain612 – 718107tRNA-binding
Motif27 – 3711"HIGH" region HAMAP MF_00098
Motif348 – 3525"KMSKS" region HAMAP MF_00098

Sites

Metal binding1581Zinc By similarity
Metal binding1611Zinc By similarity
Metal binding1711Zinc By similarity
Metal binding1741Zinc By similarity
Binding site3511ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A0K9L0 [UniParc].

Last modified December 12, 2006. Version 1.
Checksum: F355F74003839A3D

FASTA71879,227
        10         20         30         40         50         60 
MSASDLTSVQ AAAPQGSRQI LVTSALPYAN GQIHIGHLVE YIQTDIWVRT LRMHGHEVYY 

        70         80         90        100        110        120 
IGADDTHGTP IMLRAEKEGL TPKQLIDRVW TEHKRDFDSF GVSFDNFYTT DSEENRVLSE 

       130        140        150        160        170        180 
NIYLALQEAG LIAEREIEQA YDPVKEMFLP DRFIKGECPK CHAKDQYGDS CEVCGSTYLP 

       190        200        210        220        230        240 
TELLNPYSVV SGATPVRKTS KHYFFRLSDP RCESFLREWV SGLAQPEATN KMREWLGDAG 

       250        260        270        280        290        300 
ESKLADWDIS RDAPYFGFEI PGAPGKYFYV WLDAPVGYYA SFKNLCERNG IDFDAWIRPG 

       310        320        330        340        350        360 
SKAEQYHFIG KDILYFHTLF WPAMLEFSGH RTPTNVFAHG FLTVDGAKMS KSRGTFITAQ 

       370        380        390        400        410        420 
SYIDTGLNPE WLRYYFAAKL NATMEDIDLN LDDFQARVNS DLVGKYVNIA SRAAGFLIKR 

       430        440        450        460        470        480 
FDGRVQDSAM NHPLVAKLRD AIPQIAASYE AREYGRALRH TMELADEVNA YVDGAKPWDL 

       490        500        510        520        530        540 
AKDPANAVAL HETCSVSLEA FRLLSLALKP VMPRVAVAVE AFFGIAPLAW ADAAKPLSSA 

       550        560        570        580        590        600 
QPIKAYQHLM TRVDAKQIEA LLAANRDSLQ AEAAGAAAAG ANAAKDAKSN AKASAKPAAV 

       610        620        630        640        650        660 
NGADDAPISI DDFAKIDLRI AKIVACQAVE GSDKLLQLTL DVGEEKTRNV FSGIKSAYQP 

       670        680        690        700        710 
EQLVGKLTVM VANLAPRKMK FGLSEGMVLA ASATDEKAEP GLYILEPHSG AKPGMRVK

« Hide

References

[1]"Complete sequence of chromosome 1 of Burkholderia cenocepacia HI2424."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Kim E., LiPuma J.J., Gonzalez C.F., Konstantinidis K., Tiedje J.M., Richardson P.
Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HI2424.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000458 Genomic DNA. Translation: ABK09187.1.
RefSeqYP_836080.1. NC_008542.1.

3D structure databases

ProteinModelPortalA0K9L0.
SMRA0K9L0. Positions 17-566.
ModBaseSearch...

Protein-protein interaction databases

STRINGA0K9L0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4448985.
GenomeReviewsGene locus Bcen2424_2437 in contig CP000458_GR.
KEGGbch:Bcen2424_2437.
PATRIC19062294. VBIBurCen15205_2486.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0143.
HOGENOMHBG721667.
OMAEEKTRNV.
ProtClustDBPRK00133.

Enzyme and pathway databases

BioCycBCEN331272:BCEN2424_2437-MONOMER.

Family and domain databases

HAMAPMF_00098. Met_tRNA_synth_type1.
[Tree]
InterProIPR015413. aa-tRNA-synt_I.
IPR001412. aa-tRNA-synth_I_CS.
IPR004495. Met-tRNA-synth_Ia_bsu_C.
IPR014758. Met-tRNA_synth.
IPR023458. Met-tRNA_synth_1.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KOK01874.
PfamPF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSPR01041. TRNASYNTHMET.
SUPFAMSSF50249. Nucleic_acid_OB. 1 hit.
SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMsTIGR00398. MetG. 1 hit.
TIGR00399. MetG_C_term. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYM_BURCH
AccessionPrimary (citable) accession number: A0K9L0
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: December 12, 2006
Last modified: January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents