A0K8U2 (HUTI_BURCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Imidazolonepropionase
EC=3.5.2.7

Alternative name(s):
Imidazolone-5-propionate hydrolase

Gene names

Name:	hutI
Ordered Locus Names:	Bcen2424_2168

Organism

Burkholderia cenocepacia (strain HI2424) [Complete proteome] [HAMAP]

Taxonomic identifier

331272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	407 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H2O = N-formimidoyl-L-glutamate + H+. HAMAP MF_00372
Cofactor	Binds 1 zinc or iron ion per subunit By similarity. HAMAP MF_00372
Pathway	Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 3/3. HAMAP MF_00372
Subcellular location	Cytoplasm Potential HAMAP MF_00372.
Sequence similarities	Belongs to the HutI family.

Ontologies

Keywords
Biological process	Histidine metabolism
Cellular component	Cytoplasm
Ligand	Iron Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	histidine catabolic process to glutamate and formamide Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	imidazolonepropionase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 407	407	Imidazolonepropionase HAMAP MF_00372		PRO_0000306447
Sites
	Metal binding	68	1	Zinc or iron By similarity
	Metal binding	70	1	Zinc or iron By similarity
	Metal binding	238	1	Zinc or iron By similarity
	Metal binding	313	1	Zinc or iron By similarity
	Binding site	77	1	Substrate By similarity
	Binding site	90	1	Substrate By similarity
	Binding site	140	1	Substrate By similarity
	Binding site	173	1	Substrate By similarity
	Binding site	241	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0K8U2 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 4957FACB2984A362	FASTA	407	44,092
	10 20 30 40 50 60 MKPTVWHHLR LCPHGHPDET IDDAAIAVDE TGTIAWLGAL SALPHGYAHW QREDLHGAWV 70 80 90 100 110 120 TPGLVDCHTH LVYGGTRADE FAQRLAGVSY EEIARQGGGI VSTVRATRAA DETTLFVQAA 130 140 150 160 170 180 ARLQPLLAEG VTAIEIKSGY GLDLASERKM LRVARQLGER FPVTVYTTFL GAHALPPEYA 190 200 210 220 230 240 GRADEYIDEV CDRMLPTLAD EGLVDAVDVF CERIGFSLAQ TERVFEAATR RGLPVKLHAE 250 260 270 280 290 300 QLSNAGGTAL AARYRALSAD HLEFLDEAGI EAMKAAGTVA VLLPGAYYFI RETQLPPIDL 310 320 330 340 350 360 LRKHGVPIAL ATDHNPGTSP LESLLLTLNM GCTLFRMTVP EVLQGVTRHA AAALGRADRH 370 380 390 400 GALEVGRQAD FAAWSVGSLA ELAYWIGRPL CEQVVRGGTP VFRRMNG

References

[1]

"Complete sequence of chromosome 1 of Burkholderia cenocepacia HI2424." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., LiPuma J.J., Gonzalez C.F., Konstantinidis K., Tiedje J.M., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HI2424.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000458 Genomic DNA. Translation: ABK08919.1.
RefSeq	YP_835812.1. NC_008542.1.
3D structure databases
ProteinModelPortal	A0K8U2.
SMR	A0K8U2. Positions 4-401.
ModBase	Search...
Protein-protein interaction databases
STRING	A0K8U2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4449985.
GenomeReviews	Gene locus Bcen2424_2168 in contig CP000458_GR.
KEGG	bch:Bcen2424_2168.
PATRIC	19061715. VBIBurCen15205_2207.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1228.
HOGENOM	HBG686142.
OMA	MNMACTL.
ProtClustDB	PRK09356.
Enzyme and pathway databases
BioCyc	BCEN331272:BCEN2424_2168-MONOMER.
Family and domain databases
HAMAP	MF_00372. HutI. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR005920. HutI. IPR011059. Metal-dep_hydrolase_composite. [Graphical view]
KO	K01468.
PANTHER	PTHR22642. PTHR22642. 1 hit.
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
SUPFAM	SSF51338. Metalo_hydrolase. 1 hit.
TIGRFAMs	TIGR01224. HutI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HUTI_BURCH

Accession

Primary (citable) accession number: A0K8U2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	December 12, 2006
Last modified:	January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program