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A0K6X5 (IF2_BURCH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Translation initiation factor IF-2
Gene names
Name:infB
Ordered Locus Names:Bcen2424_1500
OrganismBurkholderia cenocepacia (strain HI2424) [Complete proteome] [HAMAP]
Taxonomic identifier331272 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex

Protein attributes

Sequence length971 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex By similarity. HAMAP-Rule MF_00100

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00100.

Sequence similarities

Belongs to the IF-2 family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandGTP-binding
Nucleotide-binding
   Molecular functionInitiation factor
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

GTPase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

translation initiation factor activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 971971Translation initiation factor IF-2 HAMAP-Rule MF_00100
PRO_1000008209

Regions

Nucleotide binding480 – 4878GTP By similarity
Nucleotide binding526 – 5305GTP By similarity
Nucleotide binding580 – 5834GTP By similarity
Region474 – 622149G-domain HAMAP-Rule MF_00100

Sequences

Sequence LengthMass (Da)Tools
A0K6X5 [UniParc].

Last modified December 12, 2006. Version 1.
Checksum: 90A5E7DB5D59FA23

FASTA971104,278
        10         20         30         40         50         60 
MASNNVAQFA AELKMPAGVL LEQLQAAGVQ KASEDDALSE TDKARLLDHL RKSHGATDGD 

        70         80         90        100        110        120 
KRKITLTRKH TSEIKQSDAT GKARTIQVEV RKKRTFVKRD DVAEGADQGQ AQVAEADDDA 

       130        140        150        160        170        180 
ELKRREEEAR REAELLEKQA QELRERQERL EREEAERRAR EEAAEAERRR AEEEAATKRA 

       190        200        210        220        230        240 
AAEVAAAQQQ AAAQQAAAEQ EATPTQSAQD EARAAAERAA QREAAKKAED AAREAADKAR 

       250        260        270        280        290        300 
AEQEEISKRR AAAEAEARAI REMMNTPRKA VVKAVEPPKP VEPPKPAEAK GTLHKPAKPE 

       310        320        330        340        350        360 
GAQARPAVKK PAGAAAPATT QAPAGAGDRN KKPGAGKGGW QDDAAKRRGI KTRGDSSGGV 

       370        380        390        400        410        420 
DRGWRGGPKG RGRHQDSSTF QAPTEPIVRE VHVPETVSVA DLAHKMSIKA SEVIKVMMKM 

       430        440        450        460        470        480 
GQMVTINQVL DQETAMIIVE ELGHRAVAAK LDDPEALLVE GESGTDAEQL PRPPVVTVMG 

       490        500        510        520        530        540 
HVDHGKTSLL DHIRRAKVAA GEAGGITQHI GAYHVDTPRG VITFLDTPGH EAFTAMRARG 

       550        560        570        580        590        600 
AKATDIVVLV VAADDGVMPQ TKEAIAHAKA GGVPIVVAIN KIDKPEANPD RVKQELVAEG 

       610        620        630        640        650        660 
VVPEEYGGDS PFVPVSAKTG AGIDDLLENV LLQAEVLELK APVEAPAKGI VIEAKLDKGK 

       670        680        690        700        710        720 
GPVATILVQS GTLNRGDIVL AGTAYGRVRA MLDENGKPTK EAGPSIPVEI QGLSEVPGAG 

       730        740        750        760        770        780 
EEVIVLPDER KAREIALFRQ GKFRDVKLAK QQAAKLESML EQMGEGEVQN LPLIIKADVQ 

       790        800        810        820        830        840 
GSQEALVQSL LKLSTDEVRV QIVHSAVGGI SENDVNLATA SKAVIIGFNT RADAQARKLA 

       850        860        870        880        890        900 
EANGIDIRYY NIIYDAVDEV KAAMSGMLAP EKREVITGMV EVRQVFKVPK IGTVAGCMVT 

       910        920        930        940        950        960 
DGIVKRSSSV RVLRNNVVIF TGELESLKRF KDDVKEVKQG FECGMSVKNF NDVTEGDQFE 

       970 
VFEVTEVART L 

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References

[1]"Complete sequence of chromosome 1 of Burkholderia cenocepacia HI2424."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Kim E., LiPuma J.J., Gonzalez C.F., Konstantinidis K., Tiedje J.M., Richardson P.
Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HI2424.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000458 Genomic DNA. Translation: ABK08252.1.
RefSeqYP_835145.1. NC_008542.1.

3D structure databases

ProteinModelPortalA0K6X5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING331272.Bcen2424_1500.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABK08252; ABK08252; Bcen2424_1500.
GeneID4448619.
KEGGbch:Bcen2424_1500.
PATRIC19060271. VBIBurCen15205_1507.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0532.
HOGENOMHOG000076907.
KOK02519.
OMADATGKTR.
OrthoDBEOG67HJSV.

Enzyme and pathway databases

BioCycBCEN331272:GHR7-1541-MONOMER.

Family and domain databases

Gene3D3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
HAMAPMF_00100_B. IF_2_B.
InterProIPR009061. DNA-bd_dom_put.
IPR000795. EF_GTP-bd_dom.
IPR013575. IF2_assoc_dom_bac.
IPR006847. IF2_N.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000178. TF_IF2_bacterial-like.
IPR015760. TIF_IF2.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PANTHERPTHR23115:SF41. PTHR23115:SF41. 1 hit.
PfamPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 2 hits.
PF11987. IF-2. 1 hit.
PF08364. IF2_assoc. 1 hit.
PF04760. IF2_N. 2 hits.
[Graphical view]
SUPFAMSSF46955. SSF46955. 1 hit.
SSF50447. SSF50447. 2 hits.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00487. IF-2. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEPS01176. IF2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameIF2_BURCH
AccessionPrimary (citable) accession number: A0K6X5
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: December 12, 2006
Last modified: May 14, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Translation initiation factors

List of translation initiation factor entries