A0K6B2 (A0K6B2_BURCH) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Hydroxyacylglutathione hydrolase HAMAP MF_01374
EC=3.1.2.6 HAMAP MF_01374

Alternative name(s):
Glyoxalase II HAMAP MF_01374

Gene names

Name:	gloB HAMAP MF_01374
Ordered Locus Names:	Bcen2424_1287

Organism

Burkholderia cenocepacia (strain HI2424) [Complete proteome] [HAMAP]

Taxonomic identifier

331272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. HAMAP MF_01374 SAAS SAAS017782
Catalytic activity	S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP MF_01374 SAAS SAAS017782
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_01374 SAAS SAAS017782
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP MF_01374
Subunit structure	Monomer By similarity. HAMAP MF_01374
Sequence similarities	Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family. HAMAP MF_01374

Ontologies

Keywords
Ligand	Metal-binding HAMAP MF_01374 SAAS SAAS017782 Zinc HAMAP MF_01374 SAAS SAAS017782
Molecular function	Hydrolase HAMAP MF_01374 SAAS SAAS017782 EMBL ABK08039.1
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	hydroxyacylglutathione hydrolase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	55	1	Zinc 1 By similarity HAMAP MF_01374
	Metal binding	57	1	Zinc 1 By similarity HAMAP MF_01374
	Metal binding	59	1	Zinc 2 By similarity HAMAP MF_01374
	Metal binding	60	1	Zinc 2 By similarity HAMAP MF_01374
	Metal binding	117	1	Zinc 1 By similarity HAMAP MF_01374
	Metal binding	146	1	Zinc 1 By similarity HAMAP MF_01374
	Metal binding	146	1	Zinc 2 By similarity HAMAP MF_01374
	Metal binding	184	1	Zinc 2 By similarity HAMAP MF_01374

Sequences

	Sequence		Length	Mass (Da)
	A0K6B2 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: DD3B2FB4B181D6A7	FASTA	273	29,247
	10 20 30 40 50 60 MNELEYVPVP AFEDNYIWLV SDGRDAIAVD PGEAAPVRRV LAERGWRLTA ILLTHHHADH 70 80 90 100 110 120 VGGVEALQHS QPADVPLTVY GPAAEAIGVV TRPLSGGDRV TLDAPAVAFD VLDVPGHTRG 130 140 150 160 170 180 HIAYFQAAGQ GAAGQGNAVP HVFCGDTLFS CGCGRLFEGT PAQMLASLDA LAALPGDTRV 190 200 210 220 230 240 HCAHEYTLSN IRFALACEPG NAALAAWRDD AQALRARGVP TLPTTIAHER AVNPFMRADS 250 260 270 AAIHATLEAE LHETVTDRLA AFTLMREWKN RFR

References

[1]

"Complete sequence of chromosome 1 of Burkholderia cenocepacia HI2424." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., LiPuma J.J., Gonzalez C.F., Konstantinidis K., Tiedje J.M., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000458 Genomic DNA. Translation: ABK08039.1.
RefSeq	YP_834932.1. NC_008542.1.
3D structure databases
ProteinModelPortal	A0K6B2.
ModBase	Search...
Protein-protein interaction databases
STRING	A0K6B2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4450979.
GenomeReviews	Gene locus Bcen2424_1287 in contig CP000458_GR.
KEGG	bch:Bcen2424_1287.
PATRIC	19059837. VBIBurCen15205_1296.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0491.
HOGENOM	HBG753931.
OMA	YVYRASN.
ProtClustDB	CLSK930603.
Family and domain databases
HAMAP	MF_01374. Glyoxalase_2. [Tree]
InterPro	IPR001279. Beta-lactamas-like. IPR017782. Hydroxyacylglutathione_Hdrlase. [Graphical view]
KO	K01069.
PANTHER	PTHR11935:SF7. PTHR11935:SF7. 1 hit.
Pfam	PF00753. Lactamase_B. 1 hit. [Graphical view]
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
TIGRFAMs	TIGR03413. GSH_gloB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A0K6B2_BURCH

Accession

Primary (citable) accession number: A0K6B2

Entry history

Integrated into UniProtKB/TrEMBL:	December 12, 2006
Last sequence update:	December 12, 2006
Last modified:	December 14, 2011
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)