A0K610 (ASTE_BURCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Succinylglutamate desuccinylase
EC=3.5.1.96

Gene names

Name:	astE
Ordered Locus Names:	Bcen2424_1185

Organism

Burkholderia cenocepacia (strain HI2424) [Complete proteome] [HAMAP]

Taxonomic identifier

331272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	342 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Transforms N(2)-succinylglutamate into succinate and glutamate By similarity. HAMAP MF_00767
Catalytic activity	N-succinyl-L-glutamate + H2O = succinate + L-glutamate. HAMAP MF_00767
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00767
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 5/5. HAMAP MF_00767
Sequence similarities	Belongs to the AspA/AstE family. Succinylglutamate desuccinylase subfamily.

Ontologies

Keywords
Biological process	Arginine metabolism
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine catabolic process to glutamate Inferred from electronic annotation. Source: InterPro
Molecular function	hydrolase activity, acting on ester bonds Inferred from electronic annotation. Source: InterPro succinylglutamate desuccinylase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 342	342	Succinylglutamate desuccinylase HAMAP MF_00767		PRO_1000017320
Sites
	Active site	222	1	Potential
	Metal binding	64	1	Zinc By similarity
	Metal binding	67	1	Zinc By similarity
	Metal binding	159	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0K610 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 2508CEF510A9243A	FASTA	342	36,199
	10 20 30 40 50 60 MPAAALLDDF LAFTLAGDAP AERDGACAGG AVRWQWLGDG LLAFEPAATD AAARASVLVS 70 80 90 100 110 120 AGVHGDETAP IELLSMLARD LVAGALPLAC RLLVVLGNVP AMRAGERYLD DDLNRLFSGR 130 140 150 160 170 180 HAQVPASREA PRAAQLEAAA SAFFGAAPAG SARWHIDMHT AIRASVFEQF ALLPHTGTPP 190 200 210 220 230 240 TRAMVEWLGD ARIAAVLLHT AKGNTYSHFT AEHCGALACT LELGKVRPFG QNDLTRFAPA 250 260 270 280 290 300 DRAVRKLVSG GRAEGGATLP RVFTVIDQIT KQSDALELFV AADVANFTAF TRGTVLAQDG 310 320 330 340 DYRYTVKHDE ERIVFPNPTV KPGLRAGLLV VDTTRDTLAA LV

References

[1]

"Complete sequence of chromosome 1 of Burkholderia cenocepacia HI2424." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., LiPuma J.J., Gonzalez C.F., Konstantinidis K., Tiedje J.M., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HI2424.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000458 Genomic DNA. Translation: ABK07937.1.
RefSeq	YP_834830.1. NC_008542.1.
3D structure databases
ProteinModelPortal	A0K610.
ModBase	Search...
Protein-protein interaction databases
STRING	A0K610.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4448253.
GenomeReviews	Gene locus Bcen2424_1185 in contig CP000458_GR.
KEGG	bch:Bcen2424_1185.
PATRIC	19059632. VBIBurCen15205_1193.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2988.
HOGENOM	HBG298271.
OMA	RWRWLGH.
ProtClustDB	PRK05324.
Enzyme and pathway databases
BioCyc	BCEN331272:BCEN2424_1185-MONOMER.
Family and domain databases
HAMAP	MF_00767. Arg_catab_AstE. [Tree]
InterPro	IPR007036. Aste_AspA. IPR016681. SuccinylGlu_desuccinylase. [Graphical view]
KO	K05526.
Pfam	PF04952. AstE_AspA. 1 hit. [Graphical view]
PIRSF	PIRSF017020. AstE. 1 hit.
TIGRFAMs	TIGR03242. Arg_catab_astE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ASTE_BURCH

Accession

Primary (citable) accession number: A0K610

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	December 12, 2006
Last modified:	December 14, 2011
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program