ID   ASTB_BURCH              Reviewed;         446 AA.
AC   A0K609;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   12-DEC-2006, sequence version 1.
DT   16-JUN-2009, entry version 16.
DE   RecName: Full=N-succinylarginine dihydrolase;
DE            EC=3.5.3.23;
GN   Name=astB; OrderedLocusNames=Bcen2424_1184;
OS   Burkholderia cenocepacia (strain HI2424).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Burkholderia; Burkholderia cepacia complex.
OX   NCBI_TaxID=331272;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Pitluck S., Chain P.,
RA   Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Kim E., LiPuma J.J., Gonzalez C.F.,
RA   Konstantinidis K., Tiedje J.M., Richardson P.;
RT   "Complete sequence of chromosome 1 of Burkholderia cenocepacia
RT   HI2424.";
RL   Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the hydrolysis of N(2)-succinylarginine into
CC       N(2)-succinylornithine, ammonia and CO(2) (By similarity).
CC   -!- CATALYTIC ACTIVITY: N(2)-succinyl-L-arginine + 2 H(2)O = N(2)-
CC       succinyl-L-ornithine + 2 NH(3) + CO(2).
CC   -!- PATHWAY: Amino-acid degradation; L-arginine degradation via AST
CC       pathway; L-glutamate and succinate from L-arginine: step 2/5.
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SIMILARITY: Belongs to the succinylarginine dihydrolase family.
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DR   EMBL; CP000458; ABK07936.1; -; Genomic_DNA.
DR   RefSeq; YP_834829.1; -.
DR   GeneID; 4448252; -.
DR   GenomeReviews; CP000458_GR; Bcen2424_1184.
DR   KEGG; bch:Bcen2424_1184; -.
DR   HOGENOM; A0K609; -.
DR   OMA; A0K609; HFAHHPA.
DR   GO; GO:0009015; F:N-succinylarginine dihydrolase activity; IEA:HAMAP.
DR   GO; GO:0019544; P:arginine catabolic process to glutamate; IEA:HAMAP.
DR   HAMAP; MF_01172; -; 1.
DR   InterPro; IPR007079; SuccinylArg_d-Hdrlase_AstB.
DR   Gene3D; G3DSA:3.75.10.20; SuccinylArg_di_hydro; 1.
DR   Pfam; PF04996; AstB; 1.
DR   TIGRFAMs; TIGR03241; arg_catab_astB; 1.
PE   3: Inferred from homology;
KW   Arginine metabolism; Complete proteome; Hydrolase.
FT   CHAIN         1    446       N-succinylarginine dihydrolase.
FT                                /FTId=PRO_1000065714.
FT   REGION       19     28       Substrate binding (By similarity).
FT   REGION      137    138       Substrate binding (By similarity).
FT   ACT_SITE    174    174       By similarity.
FT   ACT_SITE    249    249       By similarity.
FT   ACT_SITE    370    370       Nucleophile (By similarity).
FT   BINDING     110    110       Substrate (By similarity).
FT   BINDING     213    213       Substrate (By similarity).
FT   BINDING     251    251       Substrate (By similarity).
FT   BINDING     364    364       Substrate (By similarity).
SQ   SEQUENCE   446 AA;  48327 MW;  15E1E87E4AB138B7 CRC64;
     MNAQEANFDG LVGPTHNYAG LSFGNVASLN NEKSAANPKA AAKQGLRKMK QLADLGFAQG
     VLPPQERPSL RLLRELGFSG KDADVIAKAA KQAPELLAAA SSASAMWTAN AATVSPSADT
     SDGRVHFTPA NLCSKLHRAI EHEATRRTLS TLFADPTHFA VHEALTGTPA LGDEGAANHT
     RFCAEYGKPG IEFFVYGRAE YRRGPEPKRF PARQTFEASR AVAHRHGLAE EATVYAQQDP
     DVIDAGVFHN DVISVGNRDT LFTHERAFVN KQAIYDTLTA ALDARGARLN VIEVPDAAVS
     VNDAVTSYLF NSQLLSRADG SQVLVVPQEC RENARVAAYL DQLAAGNGPI HDVLVFDLRE
     SMKNGGGPAC LRLRVVLNDA ERAAVTSNVW INDTLFASLD AWIDKHYRDR LAPEDLADPA
     LLDESRTALD ELTQILRVGS LYDFQR
//