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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Burkholderia cenocepacia (strain HI2424)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei86 – 861S-adenosyl-L-methionineUniRule annotation
Binding sitei111 – 1111S-adenosyl-L-methionineUniRule annotation
Binding sitei138 – 1381S-adenosyl-L-methionineUniRule annotation
Active sitei161 – 1611By similarity
Binding sitei161 – 1611S-adenosyl-L-methionineUniRule annotation
Binding sitei165 – 1651SubstrateUniRule annotation
Binding sitei197 – 1971SubstrateUniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciBCEN331272:GHR7-942-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:Bcen2424_0918
OrganismiBurkholderia cenocepacia (strain HI2424)
Taxonomic identifieri331272 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
ProteomesiUP000000776 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 255255tRNA (guanine-N(7)-)-methyltransferasePRO_0000288128Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi331272.Bcen2424_0918.

Structurei

3D structure databases

ProteinModelPortaliA0K594.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni232 – 2354Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073968.
KOiK03439.
OMAiAQHMLEV.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0K594-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMHDDPNEAG LPPHNDAIPD ETAEGADEVN PLHHRRIRSF VTRAGRVSTG
60 70 80 90 100
QRRALDEFGP RFVVPYAPEM PDWDAIFGRS APRILEIGFG MGASTAEIAA
110 120 130 140 150
HRPSDDFLGV EVHEPGVGAL LKLIGEQGLS NIRIIQHDAV EVLEHMIAPA
160 170 180 190 200
SLDGVHIFFP DPWHKARHHK RRLIQPPLVA HLASRLKPGA YLHCATDWQN
210 220 230 240 250
YAEQMLEVLG AEPTLENTAA DYAPRPDYRP VTKFERRGLR LGHGVWDLVF

RKRAD
Length:255
Mass (Da):28,528
Last modified:December 12, 2006 - v1
Checksum:iE404FEC2378520F6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000458 Genomic DNA. Translation: ABK07671.1.
RefSeqiWP_011544784.1. NC_008542.1.
YP_834564.1. NC_008542.1.

Genome annotation databases

EnsemblBacteriaiABK07671; ABK07671; Bcen2424_0918.
KEGGibch:Bcen2424_0918.
PATRICi19059060. VBIBurCen15205_0921.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000458 Genomic DNA. Translation: ABK07671.1.
RefSeqiWP_011544784.1. NC_008542.1.
YP_834564.1. NC_008542.1.

3D structure databases

ProteinModelPortaliA0K594.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi331272.Bcen2424_0918.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK07671; ABK07671; Bcen2424_0918.
KEGGibch:Bcen2424_0918.
PATRICi19059060. VBIBurCen15205_0921.

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073968.
KOiK03439.
OMAiAQHMLEV.
OrthoDBiEOG6K6VBC.

Enzyme and pathway databases

UniPathwayiUPA00989.
BioCyciBCEN331272:GHR7-942-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome 1 of Burkholderia cenocepacia HI2424."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.
    , Kim E., LiPuma J.J., Gonzalez C.F., Konstantinidis K., Tiedje J.M., Richardson P.
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HI2424.

Entry informationi

Entry nameiTRMB_BURCH
AccessioniPrimary (citable) accession number: A0K594
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: December 12, 2006
Last modified: April 1, 2015
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.