A0K576 (URE1_BURCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Urease subunit alpha
EC=3.5.1.5

Alternative name(s):
Urea amidohydrolase subunit alpha

Gene names

Name:	ureC
Ordered Locus Names:	Bcen2424_0900

Organism

Burkholderia cenocepacia (strain HI2424) [Complete proteome] [HAMAP]

Taxonomic identifier

331272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	568 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Urea + H2O = CO2 + 2 NH3. HAMAP MF_01953
Cofactor	Binds 2 nickel ions per subunit By similarity. HAMAP MF_01953
Pathway	Nitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (urease route): step 1/1. HAMAP MF_01953
Subunit structure	Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_01953.
Post-translational modification	Carbamylation allows a single lysine to coordinate two nickel ions By similarity. HAMAP MF_01953
Sequence similarities	Belongs to the urease family. Contains 1 urease domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Nickel
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	urea metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nickel cation binding Inferred from electronic annotation. Source: InterPro urease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 568	568	Urease subunit alpha HAMAP MF_01953		PRO_1000073699
Regions
	Domain	130 – 568	439	Urease
Sites
	Active site	321	1	Proton donor By similarity
	Metal binding	135	1	Nickel 2 By similarity
	Metal binding	137	1	Nickel 2 By similarity
	Metal binding	218	1	Nickel 1; via carbamate group By similarity
	Metal binding	218	1	Nickel 2; via carbamate group By similarity
	Metal binding	247	1	Nickel 1 By similarity
	Metal binding	273	1	Nickel 1 By similarity
	Metal binding	361	1	Nickel 2 By similarity
	Binding site	220	1	Substrate By similarity
Amino acid modifications
	Modified residue	218	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0K576 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 2EC22BEBF4A0C9BC	FASTA	568	60,957
	10 20 30 40 50 60 MTLRLSRRAY AEMFGPTTGD RVRLADTELL IEIERDFTTY GEEVKFGGGK VIRDGMGQSQ 70 80 90 100 110 120 RVAADVPDTV ITNAVILDHW GIVKADIAIK HGRIAAIGKA GNPDIQPGVT IAIGAATEVI 130 140 150 160 170 180 AGEGLIVTAG GIDTHIHFIS PQQIDEALAS GVTTMLGGGT GPATGTNATT CTPGPWHMER 190 200 210 220 230 240 MLQAADGWPI NLGFLGKGNA SLPQPLVEQI AAGAIGLKLH EDWGTTPAAI DNCLSVADDT 250 260 270 280 290 300 DTQVAIHTDT LNEAGFVEST VAAFKGRTIH TYHTEGAGGG HAPDILKVCG EMNVLPSSTN 310 320 330 340 350 360 PTRPYTINTL DEHLDMLMVC HHLDPSIAED LAFAESRIRR ETIAAEDILH DLGALSMLSS 370 380 390 400 410 420 DSQAMGRVGE VIIRTWQTAH KMKVQRGALP EDTARNDNFR AKRYVAKYTI NPALTHGIAH 430 440 450 460 470 480 EVGSIEPGKW ADLVLWEPAF FGIKPSMILK GGMIAVAQMG DPNASIPTPQ PVHYREMFAT 490 500 510 520 530 540 RGGALARTSL TFVSQMAADA GIAERYGLAK RIVPVRNCRN VTKADMIHNA WRPSISVDPE 550 560 TYDVIADGQL LTCEPATVLP MAQRYFLF

References

[1]

"Complete sequence of chromosome 1 of Burkholderia cenocepacia HI2424." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., LiPuma J.J., Gonzalez C.F., Konstantinidis K., Tiedje J.M., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HI2424.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000458 Genomic DNA. Translation: ABK07653.1.
RefSeq	YP_834546.1. NC_008542.1.
3D structure databases
ProteinModelPortal	A0K576.
SMR	A0K576. Positions 5-568.
ModBase	Search...
Protein-protein interaction databases
STRING	A0K576.
Protein family/group databases
MEROPS	M38.982.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4449649.
GenomeReviews	Gene locus Bcen2424_0900 in contig CP000458_GR.
KEGG	bch:Bcen2424_0900.
PATRIC	19059026. VBIBurCen15205_0904.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0804.
HOGENOM	HBG357507.
OMA	TIHAFHT.
ProtClustDB	PRK13207.
Enzyme and pathway databases
BioCyc	BCEN331272:BCEN2424_0900-MONOMER.
Family and domain databases
HAMAP	MF_01953. Urease_alpha. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR011059. Metal-dep_hydrolase_composite. IPR011612. Urease_alpha_N. IPR005848. Urease_asu. IPR017951. Urease_asu_c. IPR017950. Urease_asu_CS. [Graphical view]
KO	K01428.
Pfam	PF01979. Amidohydro_1. 1 hit. PF00449. Urease_alpha. 1 hit. [Graphical view]
PRINTS	PR01752. UREASE.
SUPFAM	SSF51338. Metalo_hydrolase. 2 hits.
TIGRFAMs	TIGR01792. Urease_alph. 1 hit.
PROSITE	PS01120. UREASE_1. 1 hit. PS00145. UREASE_2. 1 hit. PS51368. UREASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

URE1_BURCH

Accession

Primary (citable) accession number: A0K576

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	December 12, 2006
Last modified:	December 14, 2011
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program