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Protein

D-(-)-3-hydroxybutyrate oligomer hydrolase

Gene

Bcen2424_0840

Organism
Burkholderia cenocepacia (strain HI2424)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Participates in the degradation of poly-3-hydroxybutyrate (PHB). It works downstream of poly(3-hydroxybutyrate) depolymerase, hydrolyzing D--3-hydroxybutyrate oligomers of various length (3HB-oligomers) into 3HB-monomers.UniRule annotation

Catalytic activityi

(R)-3-((R)-3-hydroxybutanoyloxy)butanoate + H2O = 2 (R)-3-hydroxybutanoate.UniRule annotation

Pathwayi: butanoate metabolism

This protein is involved in the pathway butanoate metabolism, which is part of Lipid metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway butanoate metabolism and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei309 – 3091Charge relay systemUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciBCEN331272:GHR7-864-MONOMER.
UniPathwayiUPA00863.

Names & Taxonomyi

Protein namesi
Recommended name:
D-(-)-3-hydroxybutyrate oligomer hydrolaseUniRule annotation (EC:3.1.1.22UniRule annotation)
Short name:
3HB-oligomer hydrolaseUniRule annotation
Short name:
3HBOHUniRule annotation
Gene namesi
Ordered Locus Names:Bcen2424_0840
OrganismiBurkholderia cenocepacia (strain HI2424)
Taxonomic identifieri331272 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex

Subcellular locationi

  • Secreted UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626UniRule annotationAdd
BLAST
Chaini27 – 696670D-(-)-3-hydroxybutyrate oligomer hydrolasePRO_5000164646Add
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the D-(-)-3-hydroxybutyrate oligomer hydrolase family.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000263490.
KOiK07518.
OMAiNPEKDWG.

Family and domain databases

Gene3Di3.40.50.1820. 3 hits.
HAMAPiMF_01906. 3HBOH. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR016582. OHBut_olig_hydro_put.
[Graphical view]
PfamiPF10605. 3HBOH. 1 hit.
[Graphical view]
PIRSFiPIRSF011409. HObutyrate_olig_hydrol. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A0K516-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKLGWGRRV VWGAALAAVA MLGACNGDES AERNRLPGFV SGSVRTTAYD
60 70 80 90 100
GASDDLLTAG LGKTGLAAAT APGFANPSRP TSAELRRLAI WSNYRALVDM
110 120 130 140 150
SANGGYGRFW GPNVDLDGND TLGEGKIPGT EYLAYADDGS GSKNVTLLVQ
160 170 180 190 200
VPASFNPAQP CIVTATSSGS RGVYGAISAA GEWALKRGCA VAYNDKGGGN
210 220 230 240 250
GAHELGSDTV TLIDGTLANA VLAGTASLFT ANVTSGDLAA FNSRFPNRYA
260 270 280 290 300
FKHAHSQQNP EQDWGRVTLQ SVEFAYWALN EQFGPVIDGA RHGVRYRAGD
310 320 330 340 350
ITTIAASVSN GGGASLAAAE QDNRGWITAV VVGEPQINVR MAPNAVVRAG
360 370 380 390 400
GQPVPSFGRP LADYATLANL LEPCAAASAS LAGAPYLSAL PATTTQSIRT
410 420 430 440 450
QRCATLAAAG LVAGADTQSQ AADALAQLHA AGYLADSDLL QAPMWDSQAI
460 470 480 490 500
PAIAVTYANA YTRSRVTDNL CNFSFATTNP ATGVVAAPAA SPMPSVFGVG
510 520 530 540 550
NGVPPTAGIN LVFNDGAGTD HRLATPDASF AGALCLRQLW TNGMLGMPAN
560 570 580 590 600
VDAVRVNANL HGKPAIIVQG RSDALVPVNH ASRAYVAQNG ISEAGRSQLV
610 620 630 640 650
FYEVTNGQHF DAFLSVPGFD TRFVPVHYYN VQALNLMWRH LKNGAPLPPS
660 670 680 690
QVIRTVPRGG TPGAAPALTS ANLPPISTAP GANAIATGVG TIDVPL
Length:696
Mass (Da):71,693
Last modified:December 12, 2006 - v1
Checksum:i9C7DCC29A2FC4146
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000458 Genomic DNA. Translation: ABK07593.1.
RefSeqiWP_011544713.1. NC_008542.1.

Genome annotation databases

EnsemblBacteriaiABK07593; ABK07593; Bcen2424_0840.
KEGGibch:Bcen2424_0840.
PATRICi19058901. VBIBurCen15205_0842.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000458 Genomic DNA. Translation: ABK07593.1.
RefSeqiWP_011544713.1. NC_008542.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK07593; ABK07593; Bcen2424_0840.
KEGGibch:Bcen2424_0840.
PATRICi19058901. VBIBurCen15205_0842.

Phylogenomic databases

HOGENOMiHOG000263490.
KOiK07518.
OMAiNPEKDWG.

Enzyme and pathway databases

UniPathwayiUPA00863.
BioCyciBCEN331272:GHR7-864-MONOMER.

Family and domain databases

Gene3Di3.40.50.1820. 3 hits.
HAMAPiMF_01906. 3HBOH. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR016582. OHBut_olig_hydro_put.
[Graphical view]
PfamiPF10605. 3HBOH. 1 hit.
[Graphical view]
PIRSFiPIRSF011409. HObutyrate_olig_hydrol. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHBOH_BURCH
AccessioniPrimary (citable) accession number: A0K516
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: December 12, 2006
Last modified: September 7, 2016
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.