A0K4T7 (PUR5_BURCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine cyclo-ligase
EC=6.3.3.1

Alternative name(s):
AIR synthase
AIRS
Phosphoribosyl-aminoimidazole synthetase

Gene names

Name:	purM
Ordered Locus Names:	Bcen2424_0761

Organism

Burkholderia cenocepacia (strain HI2424) [Complete proteome] [HAMAP]

Taxonomic identifier

331272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP MF_00741_B
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP MF_00741_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00741_B.
Sequence similarities	Belongs to the AIR synthase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine cyclo-ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 351	351	Phosphoribosylformylglycinamidine cyclo-ligase HAMAP MF_00741_B		PRO_1000046424

Sequences

	Sequence		Length	Mass (Da)
	A0K4T7 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 946F04B5951BDB5E	FASTA	351	36,779
	10 20 30 40 50 60 MNPPKSAPDA QGLSYRDAGV DIDAGDALID KIKPFAKKTL RDGVLGGIGG FGALFEVPKK 70 80 90 100 110 120 YKEPVLVSGT DGVGTKLKLA FHLNKHDTVG QDLVAMSVND ILVQGAEPLF FLDYFACGKL 130 140 150 160 170 180 DVDTAATVVK GIAQGCELSG CALIGGETAE MPGMYPDGEY DLAGFAVGAV EKSKIIDGST 190 200 210 220 230 240 IAEGDVVLGL ASSGIHSNGF SLVRKIIERA NPDLSADFHG RSLADALMAP TRIYVKPLLA 250 260 270 280 290 300 LMQKLSVKGM AHITGGGLVE NIPRVLRDGL TAELDQNAWP LPPLFKWLQE HGGVADAEMH 310 320 330 340 350 RVFNCGIGMA VIVSAADADA AIADLTAAGE QVWKIGTVRA TREGEAQTVV V

References

[1]

"Complete sequence of chromosome 1 of Burkholderia cenocepacia HI2424." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., LiPuma J.J., Gonzalez C.F., Konstantinidis K., Tiedje J.M., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HI2424.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000458 Genomic DNA. Translation: ABK07514.1.
RefSeq	YP_834407.1. NC_008542.1.
3D structure databases
ProteinModelPortal	A0K4T7.
SMR	A0K4T7. Positions 13-350.
ModBase	Search...
Protein-protein interaction databases
STRING	A0K4T7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4450790.
GenomeReviews	Gene locus Bcen2424_0761 in contig CP000458_GR.
KEGG	bch:Bcen2424_0761.
PATRIC	19058743. VBIBurCen15205_0764.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0150.
HOGENOM	HBG531222.
OMA	GIDMIAM.
ProtClustDB	PRK05385.
Enzyme and pathway databases
BioCyc	BCEN331272:BCEN2424_0761-MONOMER.
Family and domain databases
HAMAP	MF_00741_B. AIRS_B. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR004733. PurM_cligase. IPR016188. PurM_N-like. [Graphical view]
KO	K01933.
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 1 hit. SSF55326. PurM_N-like. 1 hit.
TIGRFAMs	TIGR00878. PurM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR5_BURCH

Accession

Primary (citable) accession number: A0K4T7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	December 12, 2006
Last modified:	December 14, 2011
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program