Reviewed, UniProtKB/Swiss-Prot A0K4S2 (HEMH_BURCH)

Names and origin

Protein names

Recommended name:
    Ferrochelatase
    EC=4.99.1.1
Alternative name(s):
    Protoheme ferro-lyase
    Heme synthetase

Gene names

Name:	hemH
Ordered Locus Names:	Bcen2424_0746

Organism

Burkholderia cenocepacia (strain HI2424) [Complete proteome] [HAMAP]

Taxonomic identifier

331272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the ferrous insertion into protoporphyrin IX. HAMAP MF_00323
Catalytic activity	Protoheme + 2 H+ = protoporphyrin + Fe2+. HAMAP MF_00323
Pathway	Porphyrin metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1. HAMAP MF_00323
Subcellular location	Cytoplasm By similarity HAMAP MF_00323.
Sequence similarities	Belongs to the ferrochelatase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferrochelatase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	Ferrochelatase HAMAP MF_00323		PRO_1000019277
Sites
	Metal binding	214	1	Iron By similarity
	Metal binding	295	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0K4S2-1 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: DC62633D1B86D1A2	FASTA	354	39,392
	10 20 30 40 50 60 MRFDLEPPSS VAAAHRIGVL LINLGTPDAP TPRAVRRYLA EFLSDPRVVE IPQAVWQVLL 70 80 90 100 110 120 RTLILPLRGR ASAKKYAAVW MPEGSPLRVY TERQTDSVRH LLTSNGYHVM VDYAMRYGSP 130 140 150 160 170 180 NISHALTQFK RAGVERVLLM PMYPQYSAST TATTFDAAFD ALARMRNQPE VRTVRHYADH 190 200 210 220 230 240 PAYIHALAEQ VRQYWAQHGR PDFAAGDKLV LSFHGVPKRT LDLGDPYHDQ CQQTGALLMA 250 260 270 280 290 300 ALGLSTTECR VTFQSRFGKA EWLQPYTAPT LREFGEAGVR RADVFCPGFT ADCLETIEEI 310 320 330 340 350 GMEVRDEFLA GGGTTFHRIP CLNGASAWIG ALSEIVAENL QGWPVKAAQP EPVN

References

[1]

"Complete sequence of chromosome 1 of Burkholderia cenocepacia HI2424." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., LiPuma J.J., Gonzalez C.F., Konstantinidis K., Tiedje J.M., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000458 Genomic DNA. Translation: ABK07499.1.
RefSeq	YP_834392.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A0K4S2.
Genome annotation databases
GeneID	4450571.
GenomeReviews	Gene locus Bcen2424_0746 in contig CP000458_GR.
KEGG	bch:Bcen2424_0746.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0276.
HOGENOM	HBG697135.
OMA	TIEEIGM.
Family and domain databases
HAMAP	MF_00323. Ferrochelatase. [Tree]
InterPro	IPR001015. Ferrochelatase. IPR019772. Ferrochelatase_AS. [Graphical view]
PANTHER	PTHR11108. Ferrochelatase. 1 hit.
Pfam	PF00762. Ferrochelatase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00109. hemH. 1 hit.
PROSITE	PS00534. FERROCHELATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEMH_BURCH

Accession

Primary (citable) accession number: A0K4S2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	December 12, 2006
Last modified:	February 9, 2010
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)