A0K4I3 (PROA_BURCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-glutamyl phosphate reductase
Short name=GPR
EC=1.2.1.41

Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	Bcen2424_0657

Organism

Burkholderia cenocepacia (strain HI2424) [Complete proteome] [HAMAP]

Taxonomic identifier

331272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate By similarity. HAMAP MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity HAMAP MF_00412.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.
Sequence caution	The sequence ABK07410.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 423	423	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_0000340875

Sequences

	Sequence		Length	Mass (Da)
	A0K4I3 [UniParc]. Last modified June 10, 2008. Version 2. Checksum: 604ADE6D375D161A	FASTA	423	45,185
	10 20 30 40 50 60 MDIDQYMTDL GRRARHASRA MARASTAAKN AALDAVARAI ERDAQALKDA NARDVARARE 70 80 90 100 110 120 KGLDAAFIDR LTLSDKALNT MVEGLRQVAS LADPIGEIGN LKFRPSGIQV GQMRVPLGVI 130 140 150 160 170 180 GIIYESRPNV TIDAAALCLK SGNATILRGG SEALESNAAL AKLIGEGLEA AGLPQDAVQV 190 200 210 220 230 240 VATADRAAVG KLITMTEYVD VIVPRGGKSL IERLINEARV PMIKHLDGIC HVYVDDRADL 250 260 270 280 290 300 AKALTVCDNA KTHRYGTCNT METLLVASGI AAKLLPPLGK LYRDKQVELR VDAAARAVLA 310 320 330 340 350 360 DAGVGPLVDV TEEDWHTEYL APVLAIKVVD GLDAAIEHIN HYGSHHTDAI VTEDHDRAMR 370 380 390 400 410 420 FLREVDSASV MVNASTRFAD GFEFGLGAEI GISNDKLHAR GPVGLEGLTS LKYVVLGHGE GRQ

References

[1]

"Complete sequence of chromosome 1 of Burkholderia cenocepacia HI2424." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., LiPuma J.J., Gonzalez C.F., Konstantinidis K., Tiedje J.M., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HI2424.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000458 Genomic DNA. Translation: ABK07410.1. Different initiation.
RefSeq	YP_834303.1. NC_008542.1.
3D structure databases
ProteinModelPortal	A0K4I3.
ModBase	Search...
Protein-protein interaction databases
STRING	A0K4I3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4450758.
GenomeReviews	Gene locus Bcen2424_0657 in contig CP000458_GR.
KEGG	bch:Bcen2424_0657.
PATRIC	19058521. VBIBurCen15205_0653.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0014.
HOGENOM	HBG318080.
ProtClustDB	PRK00197.
Enzyme and pathway databases
BioCyc	BCEN331272:BCEN2424_0657-MONOMER.
Family and domain databases
HAMAP	MF_00412. ProA. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR000965. G-glutamylP_reductase. IPR020593. G-glutamylP_reductase_CS. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 2 hits.
KO	K00147.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
Pfam	PF00171. Aldedh. 2 hits. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR00407. ProA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_BURCH

Accession

Primary (citable) accession number: A0K4I3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	June 10, 2008
Last modified:	December 14, 2011
This is version 43 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program