Reviewed, UniProtKB/Swiss-Prot A0K484 (MURD_BURCH)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoylalanine--D-glutamate ligase
    EC=6.3.2.9
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
    D-glutamic acid-adding enzyme

Gene names

Name:	murD
Ordered Locus Names:	Bcen2424_0557

Organism

Burkholderia cenocepacia (strain HI2424) [Complete proteome] [HAMAP]

Taxonomic identifier

331272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	503 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 503	503	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_0000301417
Regions
	Nucleotide binding	129 – 135	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A0K484-1 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 59E7BFB25D406385	FASTA	503	52,851
	10 20 30 40 50 60 MFGDRQRPMV LVLGLGESGL AIARWCARHG CRLRIADTRE APPNLAALQA EGIDAEFVGG 70 80 90 100 110 120 AFTPALLDGG VEIVGLSPGL SPLEPALAAL VAAANERGVA VWGELEFFAQ ALRALGTSGY 130 140 150 160 170 180 QPKVLAITGT NGKTTTTSLT GLLCQRSGKK VAVAGNISPA MLDRLASAID ETALPDVWVL 190 200 210 220 230 240 ELSSFQLETA RTFAPDAAAI LNITQDHLDW HGSFDAYAQA KGRIFGATTT RVLNRDDAAV 250 260 270 280 290 300 MKFAPAVAAA DAPRTVTFGL NEPTQDGDYG LSRDNGIAWL VEAVDRDAPD EATTTRRRKR 310 320 330 340 350 360 DAAHTPDIAQ KRLMPADALR IRGLHNAANA LAAFALARAI DLPAAPLLHA LREYRGEAHR 370 380 390 400 410 420 VEVIATIDDV DYVDDSKGTN VGATVAALDG LAQKIVLIAG GDGKGQDFAP LVAPVARWCR 430 440 450 460 470 480 AVMLIGRDAP VIRDTLAETG VPLADHATLE AAVHAAAELA EPGDAVLLSP ACASLDMFRN 490 500 YAHRAEVFRA AVDAIAIDKG ATP

References

[1]

"Complete sequence of chromosome 1 of Burkholderia cenocepacia HI2424." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., LiPuma J.J., Gonzalez C.F., Konstantinidis K., Tiedje J.M., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000458 Genomic DNA. Translation: ABK07311.1.
RefSeq	YP_834204.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A0K484.
Genome annotation databases
GeneID	4450639.
GenomeReviews	Gene locus Bcen2424_0557 in contig CP000458_GR.
KEGG	bch:Bcen2424_0557.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	A0K484.
OMA	SEDHLDR.
Family and domain databases
HAMAP	MF_00639. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01087. murD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_BURCH

Accession

Primary (citable) accession number: A0K484

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	December 12, 2006
Last modified:	November 3, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)