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Protein

tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC

Gene

mnmC

Organism
Burkholderia cenocepacia (strain HI2424)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the last two steps in the biosynthesis of 5-methylaminomethyl-2-thiouridine (mnm5s2U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm5s2U34 to nm5s2U34, followed by the transfer of a methyl group from S-adenosyl-L-methionine to nm5s2U34, to form mnm5s2U34.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + tRNA containing 5-aminomethyl-2-thiouridine = S-adenosyl-L-homocysteine + tRNA containing 5-methylaminomethyl-2-thiouridylate.UniRule annotation

Cofactori

FADUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Oxidoreductase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

FAD, Flavoprotein, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmCUniRule annotation
Short name:
tRNA mnm(5)s(2)U biosynthesis bifunctional proteinUniRule annotation
Including the following 2 domains:
tRNA (mnm(5)s(2)U34)-methyltransferaseUniRule annotation (EC:2.1.1.61UniRule annotation)
FAD-dependent cmnm(5)s(2)U34 oxidoreductaseUniRule annotation (EC:1.5.-.-UniRule annotation)
Gene namesi
Name:mnmCUniRule annotation
Ordered Locus Names:Bcen2424_0066
OrganismiBurkholderia cenocepacia (strain HI2424)
Taxonomic identifieri331272 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003479501 – 643tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmCAdd BLAST643

Structurei

3D structure databases

ProteinModelPortaliA0K2U3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 223tRNA (mnm(5)s(2)U34)-methyltransferaseAdd BLAST223
Regioni247 – 643FAD-dependent cmnm(5)s(2)U34 oxidoreductaseAdd BLAST397

Sequence similaritiesi

In the N-terminal section; belongs to the methyltransferase superfamily. tRNA (mnm(5)s(2)U34)-methyltransferase family.UniRule annotation
In the C-terminal section; belongs to the DAO family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000218143.
KOiK15461.
OMAiKSVLCYD.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
3.50.50.60. 3 hits.
HAMAPiMF_01102. MnmC. 1 hit.
InterProiIPR006076. FAD-dep_OxRdtase.
IPR023753. FAD/NAD-binding_dom.
IPR008471. MnmC-like_methylTransf.
IPR029063. SAM-dependent_MTases.
IPR023032. tRNA_MAMT_biosynth_bifunc_MnmC.
IPR017610. tRNA_S-uridine_synth_MnmC_C.
[Graphical view]
PfamiPF01266. DAO. 1 hit.
PF05430. Methyltransf_30. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
TIGRFAMsiTIGR03197. MnmC_Cterm. 1 hit.

Sequencei

Sequence statusi: Complete.

A0K2U3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPDRLVSATL ALRDDGTLVS PEFGELHRGA SGTLARAHRT FVAGNGLPAR
60 70 80 90 100
WQRRRTFTIV TTAFGAGAGF LAAWAAWRDD PARCERLHVV AVEPHPFSRD
110 120 130 140 150
DLHRAVSHMV VDTTISADVD ALLDAWPILV PGLHRLEFDE GRVVLTLAFG
160 170 180 190 200
DTIDLLKKLV ARADAFFLDG AAASGDGIRA LAKLAGEHAT FATHAKSDDV
210 220 230 240 250
KHALGETGFT FREVDDRLVG DYAPRWRARR HEPPRALPVA ARRAIVIGAG
260 270 280 290 300
LAGCAVVERL AARGWDVTLI ERHERIASEA SGNPAGVFHP LMTRDDNVAS
310 320 330 340 350
RLTRGGFLHA LARWRALERA GHAFSRSTHG MLHLAESADD FARMRDAFDA
360 370 380 390 400
FGPPSDYATL LDADAARAHL NLPVAQGGLL FPHGGAVWPA GLCVAQYAAA
410 420 430 440 450
GERVRLLAST CVARLERRDD TWHALDDTGA TLADAPVVVL ANAGDAARLA
460 470 480 490 500
GLRHVTLQPV RGQLTLLPPG TTAPLPCPAI GDGYAVPLDD GTLLIGATFE
510 520 530 540 550
PDDTDPAMRA AGHAENLDRV RHLLPGLIGA LPDPATLRGR VAFRWVVGDR
560 570 580 590 600
LPLIGPLADE TQATANARAL GGAQARDLPR MPGLYGAFGF GSRGLVWAAL
610 620 630 640
GAELIASQLE GEPWPLEREL ADAVDPARFL IRALRARRVG SAG
Length:643
Mass (Da):68,643
Last modified:December 12, 2006 - v1
Checksum:i1995EB19CBB23120
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000458 Genomic DNA. Translation: ABK06820.1.
RefSeqiWP_011694013.1. NC_008542.1.

Genome annotation databases

EnsemblBacteriaiABK06820; ABK06820; Bcen2424_0066.
KEGGibch:Bcen2424_0066.
PATRICi19057309. VBIBurCen15205_0066.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000458 Genomic DNA. Translation: ABK06820.1.
RefSeqiWP_011694013.1. NC_008542.1.

3D structure databases

ProteinModelPortaliA0K2U3.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK06820; ABK06820; Bcen2424_0066.
KEGGibch:Bcen2424_0066.
PATRICi19057309. VBIBurCen15205_0066.

Phylogenomic databases

HOGENOMiHOG000218143.
KOiK15461.
OMAiKSVLCYD.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
3.50.50.60. 3 hits.
HAMAPiMF_01102. MnmC. 1 hit.
InterProiIPR006076. FAD-dep_OxRdtase.
IPR023753. FAD/NAD-binding_dom.
IPR008471. MnmC-like_methylTransf.
IPR029063. SAM-dependent_MTases.
IPR023032. tRNA_MAMT_biosynth_bifunc_MnmC.
IPR017610. tRNA_S-uridine_synth_MnmC_C.
[Graphical view]
PfamiPF01266. DAO. 1 hit.
PF05430. Methyltransf_30. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
TIGRFAMsiTIGR03197. MnmC_Cterm. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMNMC_BURCH
AccessioniPrimary (citable) accession number: A0K2U3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: December 12, 2006
Last modified: November 2, 2016
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.